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          pPZP212

          價格:2000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: --
          載體名稱: pPZP212
          質粒類型: 植物載體;農桿菌雙元表達載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: lac
          載體大小: 9105 bp
          5' 測序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
          3' 測序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
          載體標簽:
          載體抗性: 卡那霉素、壯觀霉素(鏈霉素)
          篩選標記: --
          克隆菌株: HB101
          宿主細胞(系): 植物細胞、農桿菌
          備注: 重組質?捎盟{白斑法篩選
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 誘導型
          病毒/非病毒: 非病毒

          >

          載體質粒圖譜和多克隆位點信息

          pPZP212載體圖譜



          pPZP212 多克隆位點

          載體簡介

          載體序列

          LOCUS       Exported                9015 bp ds-DNA     circular SYN 28-JUL-2016
          DEFINITION  Agrobacterium binary vector for plant transformation, with 
                      spectinomycin- and kanamycin-resistance genes. The MCS is reversed 
                      in pPZP211.
          ACCESSION   U10462
          VERSION     .
          KEYWORDS    pPZP212
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 9015)
            AUTHORS   Hajdukiewicz P, Svab Z, Maliga P.
            TITLE     The small, versatile pPZP family of Agrobacterium binary vectors for
                      plant transformation.
            JOURNAL   Plant Mol. Biol. 1994;25:989-94.
            PUBMED    7919218
          REFERENCE   2  (bases 1 to 9015)
            AUTHORS   .
            TITLE     Direct Submission
            JOURNAL   Exported Thursday, July 28, 2016 from SnapGene Viewer 3.1.4
                      http://www.snapgene.com
          COMMENT     The GenBank sequence was corrected by inserting a G at position 
                      2249.
          FEATURES             Location/Qualifiers
               source          1..9015
                               /organism="synthetic DNA construct"
                               /lab_host="Plant Cells"
                               /mol_type="other DNA"
               CDS             1187..1816
                               /codon_start=1
                               /product="stability protein from Pseudomonas plasmid pVS1"
                               /note="pVS1 StaA"
                               /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                               DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                               VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                               LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
               CDS             2245..3318
                               /codon_start=1
                               /product="replication protein from Pseudomonas plasmid 
                               pVS1"
                               /note="pVS1 RepA"
                               /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                               QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                               KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                               GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                               ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                               LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                               LVMRYRNLIEGEASAGS"
               rep_origin      3384..3578
                               /note="pVS1 oriV"
                               /note="origin of replication for the Pseudomonas plasmid 
                               pVS1 (Heeb et al., 2000)"
               misc_feature    3922..4062
                               /note="bom"
                               /note="basis of mobility region from pBR322"
               rep_origin      complement(4248..4836)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(5080..5871)
                               /codon_start=1
                               /gene="aadA"
                               /product="aminoglycoside adenylyltransferase"
                               /note="SmR"
                               /note="confers resistance to spectinomycin and 
                               streptomycin"
                               /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                               SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                               RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                               EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                               VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
               misc_feature    6399..6423
                               /note="LB T-DNA repeat"
                               /note="left border repeat from nopaline C58 T-DNA"
               polyA_signal    6521..6695
                               /note="CaMV poly(A) signal"
                               /note="cauliflower mosaic virus polyadenylation signal"
               CDS             complement(6749..7546)
                               /codon_start=1
                               /gene="aph(3')-II (or nptII)"
                               /product="aminoglycoside phosphotransferase from Tn5"
                               /note="NeoR/KanR"
                               /note="confers resistance to neomycin, kanamycin, and G418 
                               (Geneticin(R))"
                               /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                               SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                               QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                               ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
               promoter        complement(7606..8284)
                               /note="CaMV 35S promoter (enhanced)"
                               /note="cauliflower mosaic virus 35S promoter with a 
                               duplicated enhancer region"
               CDS             complement(8370..8603)
                               /codon_start=1
                               /gene="lacZ (truncated)"
                               /product="LacZ-alpha fragment of beta-galactosidase"
                               /note="lacZ-alpha"
                               /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                               LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
               primer_bind     8513..8529
                               /note="M13 fwd"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               misc_feature    8533..8589
                               /note="MCS"
                               /note="pUC18 multiple cloning site"
               primer_bind     complement(8599..8615)
                               /note="M13 rev"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               misc_feature    complement(8601..8603)
                               /note="ATG"
               protein_bind    8623..8639
                               /bound_moiety="lac repressor encoded by lacI"
                               /note="lac operator"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               promoter        complement(8647..8677)
                               /note="lac promoter"
                               /note="promoter for the E. coli lac operon"
               misc_feature    8878..8902
                               /note="RB T-DNA repeat"
                               /note="right border repeat from nopaline C58 T-DNA"
          ORIGIN
                  1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
                 61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
                121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
                181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
                241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
                301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
                361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
                421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
                481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
                541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
                601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
                661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
                721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
                781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
                841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
                901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
                961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
               1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
               1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
               1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
               1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
               1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
               1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
               1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
               1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
               1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
               1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
               1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
               1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
               1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
               1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
               1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
               1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
               1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
               2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
               2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
               2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
               2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
               2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
               2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
               2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
               2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
               2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
               2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
               2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
               2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
               2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
               2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
               2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
               2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
               3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
               3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
               3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
               3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
               3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
               3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
               3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
               3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
               3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
               3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
               3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
               3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
               3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
               3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
               3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
               3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
               3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
               4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
               4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
               4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
               4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
               4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
               4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
               4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
               4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
               4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
               4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
               4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
               4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
               4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
               4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
               4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
               4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
               4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aatcgcttga gttaacgccg
               5041 gcgaagcggc gtcggcttga acgaatttct agctagacat tatttgccga ctaccttggt
               5101 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
               5161 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
               5221 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
               5281 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
               5341 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
               5401 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
               5461 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
               5521 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
               5581 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
               5641 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
               5701 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
               5761 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
               5821 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccccca tgatgtttaa
               5881 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
               5941 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
               6001 aaaacatgtc ataacaagaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
               6061 cggtcaaggt tctggaccag ttgcgtgacg gcagttacgc tacttgcatt acagcttacg
               6121 aaccgaacga ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct
               6181 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
               6241 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
               6301 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
               6361 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
               6421 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt
               6481 aacgccgaat taattcgggg gatctatagg gactttaggt gatctggatt ttagtactgg
               6541 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
               6601 actaagggtt tcttatatgc tcaacacgtg agcgaaaccc tataagaacc ctaattccct
               6661 tatctgggaa ctactcacac attattatgg agaaactcga tgtcgatcga ctctagctag
               6721 aggatcgatc cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
               6781 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
               6841 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
               6901 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
               6961 tcggcaagca ggcatcgcca tgtgtcacga cgagatcctc gccgtcgggc atgcgcgcct
               7021 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
               7081 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
               7141 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
               7201 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
               7261 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
               7321 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctggagttca ttcagggcac
               7381 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
               7441 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
               7501 aagcggccgg agaacctgcg tgcaatccat cttgttcaat ccccatggtc gatcgacaga
               7561 tctgcgaatc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
               7621 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
               7681 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
               7741 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
               7801 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
               7861 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
               7921 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
               7981 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca
               8041 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
               8101 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
               8161 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
               8221 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
               8281 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
               8341 gtcgtgctcc accatgttgg caagctgctc tagcattcgc cattcaggct gcgcaactgt
               8401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
               8461 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
               8521 acggccagtg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
               8581 ctcgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
               8641 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
               8701 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
               8761 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
               8821 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
               8881 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt
               8941 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
               9001 tcccctcggg atcaa
          //
          
          

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