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      1. <pre id="q7rs8"></pre>
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          pGreenII 0800-LUC

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: --
          載體名稱: pGreenII 0800-LUC
          質粒類型: 植物表達載體
          高拷貝/低拷貝: --
          復制子: pSa
          啟動子: 35s
          載體大小: 6382 bp
          5' 測序引物及序列: 35S:GACGCACAATCCCACTATCC
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 卡那霉素
          篩選標記: --
          克隆菌株: DH5α 等
          宿主細胞(系): 植物細胞
          備注: --
          產品目錄號: --
          穩定性: --
          組成型/誘導型: --
          病毒/非病毒: --

          載體質粒圖譜和多克隆位點信息

          pGreenII 0800-LUC載體圖譜
          pGreenII 0800-LUC載體圖譜1

          載體簡介

          載體序列

          LOCUS       pGreenII 0800-LUC                6382 bp ds-DNA     circular SYN 14-SEP-2016
          DEFINITION  synthetic circular DNA
          ACCESSION   .
          VERSION     .
          KEYWORDS    Untitled 8
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 6382)
            AUTHORS   .
            TITLE     Direct Submission
            JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
                      http://www.ningxiaxinwen.com/
          FEATURES             Location/Qualifiers
               source          1..6382
                               /organism="synthetic DNA construct"
                               /mol_type="other DNA"
               misc_feature    542..564
                               /note="LB T-DNA repeat"
                               /note="left border repeat from nopaline C58 T-DNA 
                               (truncated)"
               promoter        626..971
                               /note="CaMV 35S promoter"
                               /note="strong constitutive promoter from cauliflower mosaic
                               virus"
               CDS             982..1917
                               /codon_start=1
                               /product="luciferase from the anthozoan coelenterate 
                               Renilla reniformis (sea pansy)"
                               /note="Rluc"
                               /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                               AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                               FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                               ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                               IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                               KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
               polyA_signal    1974..2150
                               /note="CaMV poly(A) signal"
                               /note="cauliflower mosaic virus polyadenylation signal"
               primer_bind     2314..2330
                               /note="M13 fwd"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               promoter        2337..2355
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               primer_bind     2381..2397
                               /note="KS primer"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               primer_bind     complement(2431..2447)
                               /note="SK primer"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               CDS             2479..4131
                               /codon_start=1
                               /gene="luc+"
                               /product="firefly luciferase"
                               /note="luciferase"
                               /note="enhanced luc+ version of the luciferase gene"
                               /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                               HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                               ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                               MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                               RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                               IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                               GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                               GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                               LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                               FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
               polyA_signal    4188..4364
                               /note="CaMV poly(A) signal"
                               /note="cauliflower mosaic virus polyadenylation signal"
               misc_feature    4374..4398
                               /note="RB T-DNA repeat"
                               /note="right border repeat from nopaline C58 T-DNA"
               rep_origin      complement(4489..5077)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(5248..6063)
                               /codon_start=1
                               /gene="aph(3')-Ia"
                               /product="aminoglycoside phosphotransferase"
                               /note="KanR"
                               /note="confers resistance to kanamycin in bacteria or G418 
                               (Geneticin(R)) in eukaryotes"
                               /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                               KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                               TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                               SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                               ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
               rep_origin      6354..407
                               /note="pSa ori"
                               /note="origin of replication from bacterial plasmid pSa"
          ORIGIN
                  1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
                 61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
                121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
                181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
                241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
                301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
                361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
                421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
                481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
                541 ttggcaggat atattgtggt gtaacgttat cgtaccccta ctccaaaaat gtcaaagata
                601 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
                661 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
                721 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
                781 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
                841 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
                901 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
                961 tggagaggac agcccaccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
               1021 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
               1081 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
               1141 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
               1201 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
               1261 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
               1321 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
               1381 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
               1441 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
               1501 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
               1561 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
               1621 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
               1681 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
               1741 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
               1801 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
               1861 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
               1921 tagccggtac gctgaaatca ccagtctctc tctacaaatc tatctctctc tattttctcc
               1981 ataaataatg tgtgagtagt ttcccgataa gggaaattag ggttcttata gggtttcgct
               2041 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
               2101 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc gataacatta
               2161 acgtttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
               2221 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
               2281 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
               2341 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
               2401 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
               2461 cggtggagat cgaattccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
               2521 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
               2581 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
               2641 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
               2701 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
               2761 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
               2821 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
               2881 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
               2941 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
               3001 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
               3061 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
               3121 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
               3181 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
               3241 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
               3301 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
               3361 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
               3421 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
               3481 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
               3541 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
               3601 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
               3661 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
               3721 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
               3781 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
               3841 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
               3901 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
               3961 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
               4021 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
               4081 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagaga
               4141 attcgctgaa atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat
               4201 aatgtgtgag tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg
               4261 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
               4321 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatccactag ccttgacagg
               4381 atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa
               4441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
               4501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
               4561 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
               4621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
               4681 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
               4741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
               4801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
               4861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
               4921 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag
               4981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
               5041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
               5101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
               5161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
               5221 tatatatgtg taacattggt ctagtgatta gaaaaactca tcgagcatca aatgaaactg
               5281 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
               5341 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
               5401 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
               5461 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
               5521 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
               5581 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
               5641 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
               5701 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg
               5761 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
               5821 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa caacattggc
               5881 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
               5941 gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
               6001 agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct
               6061 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
               6121 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat
               6181 aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg
               6241 ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat
               6301 caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga tccccatcca
               6361 acagcccgcc gtcgagcggg ct
          //
          
          
          

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                  品牌简介

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