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          pCambia2301

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: CAMBIA
          載體名稱: pCAMBIA2301
          質粒類型: 植物載體;農桿菌雙元表達載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: Lac
          載體大小: 11634 bp
          5' 測序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
          3' 測序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
          載體標簽: --
          載體抗性: 卡那霉素
          篩選標記: 新霉素(Neomycin)、GUS-6XHis
          克隆菌株: HB101等菌株
          宿主細胞(系): 植物細胞、農桿菌
          備注: --
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 誘導型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pCAMBIA2301載體圖譜



          pCAMBIA2301 多克隆位點

          載體簡介

          載體序列

          LOCUS       AF234316               11633 bp    DNA     circular SYN 24-APR-2000
          DEFINITION  Binary vector pCAMBIA-2301, complete sequence.
          ACCESSION   AF234316
          VERSION     AF234316.1  GI:7638149
          KEYWORDS    .
          SOURCE      Binary vector pCAMBIA-2301
            ORGANISM  Binary vector pCAMBIA-2301
                      other sequences; artificial sequences; vectors.
          REFERENCE   1  (sites)
            AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.
            TITLE     The small, versatile pPZP family of Agrobacterium binary vectors
                      for plant transformation
            JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
             PUBMED   7919218
          REFERENCE   2  (bases 1 to 11633)
            AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
                      Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
                      Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
                      Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
            TITLE     A comprehensive set of modular vectors for advanced manipulations
                      and efficient transformation of plants
            JOURNAL   Unpublished
            REMARK    Full description of constructs
          REFERENCE   3  (bases 1 to 11633)
            AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
                      Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
                      Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
                      Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
            TITLE     Direct Submission
            JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
                      GPO Box 3200, Canberra, ACT 2601, Australia
          FEATURES             Location/Qualifiers
               source          1..11633
                               /organism="Binary vector pCAMBIA-2301"
                               /mol_type="other DNA"
                               /db_xref="taxon:118408"
               CDS             join(2..16,207..2054)
                               /codon_start=1
                               /product="GUSA, hexaHis tagged"
                               /protein_id="AAF65404.1"
                               /db_xref="GI:7638153"
                               /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE
                               SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
                               GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
                               NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG
                               DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR
                               VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY
                               RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET
                               QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT
                               CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI
                               IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ
                               GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"
               exon            2..16
                               /note="synthetic first gus exon (modular)"
               intron          17..206
                               /note="Castorbean catalase intron; modified"
               exon            207..2054
                               /note="second gusA exon, His6"
               misc_feature    2086..2338
                               /note="nos (nopaline synthase) 3'UTR (polyA signal)"
               misc_feature    2376..2401
                               /note="right border T-DNA repeat"
               misc_feature    complement(3442..4442)
                               /note="STA region from pVS1 plasmid"
               rep_origin      complement(5035..6035)
                               /note="pVS1-REP; replication origin from pVS1"
               misc_feature    complement(6445..6705)
                               /note="bom site from pBR322"
               rep_origin      complement(6845..7125)
                               /note="pBR322 origin of replication"
               CDS             complement(7416..8210)
                               /note="aadA (kanamycin resistance) gene amplified from
                               pIG121Hm"
                               /codon_start=1
                               /product="aminoglycoside phosphotransferase"
                               /protein_id="AAF65402.1"
                               /db_xref="GI:7638151"
                               /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                               MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                               EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                               EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                               YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
               misc_feature    8635..8660
                               /note="left border repeat from C58 T-DNA"
               misc_feature    8727..8930
                               /note="CaMV 3'UTR (polyA signal)"
               CDS             complement(8968..9765)
                               /note="nptII (kanamycin resistance) gene"
                               /codon_start=1
                               /product="neomycin phosphotransferase"
                               /protein_id="AAF65403.1"
                               /db_xref="GI:7638152"
                               /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                               RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                               LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                               EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                               YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
               promoter        complement(9796..10576)
                               /note="CaMV35S2; CaMV 35S promoter, duplicated"
               promoter        10724..10778
                               /note="PlacZ; lacZ promoter"
               CDS             10806..11039
                               /codon_start=1
                               /product="LacZ alpha fragment"
                               /protein_id="AAF65401.1"
                               /db_xref="GI:7638150"
                               /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                               RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
               misc_feature    10820..10876
                               /note="pUC18 MCS; polylinker"
               promoter        11090..11627
                               /note="CaMV35S; 35S promoter from CaMV"
          ORIGIN      
                  1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
                 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
                121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
                181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
                241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
                301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
                361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
                421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
                481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
                541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
                601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
                661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
                721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
                781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
                841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
                901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
                961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
               1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
               1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
               1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
               1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
               1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
               1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
               1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
               1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
               1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
               1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
               1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
               1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
               1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
               1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
               1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
               1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
               1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
               2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
               2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
               2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
               2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
               2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
               2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
               2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
               2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
               2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
               2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
               2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
               2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
               2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
               2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
               2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
               2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
               3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
               3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
               3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
               3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
               3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
               3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
               3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
               3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
               3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
               3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
               3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
               3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
               3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
               3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
               3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
               3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
               3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
               4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
               4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
               4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
               4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
               4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
               4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
               4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
               4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
               4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
               4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
               4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
               4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
               4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
               4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
               4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
               4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
               4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
               5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
               5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
               5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
               5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
               5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
               5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
               5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
               5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
               5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
               5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
               5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
               5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
               5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
               5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
               5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
               5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
               6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
               6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
               6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
               6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
               6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
               6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
               6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
               6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
               6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
               6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
               6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
               6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
               6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
               6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
               6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
               6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
               6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
               7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
               7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
               7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
               7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
               7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
               7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
               7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
               7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
               7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
               7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
               7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
               7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
               7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
               7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
               7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
               7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
               7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
               8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
               8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
               8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
               8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
               8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
               8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
               8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
               8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
               8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
               8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
               8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
               8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
               8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
               8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
               8881 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac tctagctaga
               8941 ggatcgatcc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
               9001 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
               9061 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
               9121 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
               9181 cggcaagcag gcatcgccat gtgtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
               9241 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
               9301 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
               9361 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
               9421 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
               9481 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
               9541 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tggagttcat tcagggcacc
               9601 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
               9661 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
               9721 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc cccatggtcg atcgacagat
               9781 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
               9841 tccaaatgaa atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg
               9901 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
               9961 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
              10021 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
              10081 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
              10141 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
              10201 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc
              10261 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
              10321 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
              10381 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
              10441 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
              10501 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
              10561 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
              10621 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
              10681 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
              10741 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
              10801 cagctatgac catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct
              10861 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
              10921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
              10981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
              11041 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttagcttc atggagtcaa
              11101 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga
              11161 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac
              11221 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
              11281 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
              11341 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
              11401 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
              11461 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
              11521 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
              11581 cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt gac
          //
          

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