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          pCambia1391Z

          價格:1800元

          聯系方式:I47-825O-882O

          買家導航

          pCambia 1391Z載體基本信息

          出品公司: CAMBIA
          載體名稱: pCAMBIA1391Z
          質粒類型: 植物載體;農桿菌雙元表達載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: lac
          載體大小: 11227 bp
          5' 測序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
          3' 測序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
          載體標簽: GUS、6xHis
          載體抗性: 卡那霉素
          篩選標記: 潮霉素
          克隆菌株: HB101等菌株
          宿主細胞(系): 植物細胞、農桿菌
          備注: --
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 誘導型
          病毒/非病毒: 非病毒

          pCambia 1391Z載體質粒圖譜和多克隆位點信息

          pCAMBIA1391Z載體圖譜



          pCAMBIA1391Z 多克隆位點

          pCambia 1391Z載體簡介

          pCambia 1391Z載體序列

          LOCUS       pCAMBIA1391Z          11227 bp ds-DNA    circular SYN 01-十二月-2013
          DEFINITION  Agrobacterium binary vector for plant transformation, with 
                      hygromycin- and kanamycin-resistance and LacZ-GUS genes plus the 
                      pUC9 MCS.
          ACCESSION   AF234312
          VERSION     .
          KEYWORDS    pCAMBIA1391Z
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 11227)
            AUTHORS   Cambia
            TITLE     Direct Submission
            JOURNAL   Exported 2014年6月2日 from SnapGene Viewer 1.5.3
                      http://www.snapgene.com
          COMMENT     The GenBank record was corrected by inserting a G at position 4743.
          FEATURES             Location/Qualifiers
               source          1..11227
                               /organism="synthetic DNA construct"
                               /lab_host="Plant Cells"
                               /mol_type="other DNA"
               CDS             join(2..16,207..2024)
                               /codon_start=1
                               /product="beta-glucuronidase"
                               /label="GUS"
                               /label="This version of the gusA gene has a 5' extension 
                               with a catalase intron to ensure expression in plants but 
                               not bacteria."
                               /protein_id="
                               "                    
               intron          17..206
                               /label="cat1 intron"
                               /label="castor bean catalase intron, modified"
               CDS             2031..2048
                               /codon_start=1
                               /product="6xHis affinity tag"
                               /label="6xHis"
                               /translation="HHHHHH"
               terminator      2083..2335
                               /label="NOS terminator"
                               /label="nopaline synthase terminator and poly(A) signal"
               misc_feature    2357..2381
                               /label="RB T-DNA repeat"
                               /label="right border repeat from nopaline C58 T-DNA"
               CDS             3681..4310
                               /codon_start=1
                               /product="stability protein from Pseudomonas plasmid pVS1"
                               /label="pVS1 StaA"                     
               CDS             4739..5812
                               /codon_start=1
                               /product="replication protein from Pseudomonas plasmid 
                               pVS1"
                               /label="pVS1 RepA"
                               /label="
                               "
                               /protein_id="
                               "                     
               misc_feature    6416..6556
                               /label="bom"
                               /label="basis of mobility region from pBR322"
               rep_origin      complement(6742..7330)
                               /direction=LEFT
                               /label="ori"
                               /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(7417..8211)
                               /codon_start=1
                               /gene="aphA-3"
                               /product="aminoglycoside phosphotransferase"
                               /label="KanR"
                               /label="confers resistance to kanamycin"
                               /protein_id="
                               "
               misc_feature    8636..8660
                               /label="LB T-DNA repeat"
                               /label="left border repeat from nopaline C58 T-DNA"
               polyA_signal    8738..8912
                               /label="CaMV poly(A) signal"
                               /label="cauliflower mosaic virus polyadenylation signal"
               CDS             complement(8952..9977)
                               /codon_start=1
                               /product="hygromycin B phosphotransferase"
                               /label="HygR"
                               /label="confers resistance to hygromycin"                     
               promoter        complement(10045..10722)
                               /label="CaMV 35S promoter (enhanced)"
                               /label="cauliflower mosaic virus 35S promoter with a 
                               duplicated enhancer region"
               promoter        10936..10966
                               /label="lac promoter"
                               /label="promoter for the E. coli lac operon"
               protein_bind    10974..10990
                               /bound_moiety="lac repressor encoded by lacI"
                               /label="lac operator"
                               /label="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               primer_bind     10998..11014
                               /label="M13 rev"
                               /label="common sequencing primer, one of multiple similar 
                               variants"
               CDS             11010..11207
                               /codon_start=1
                               /gene="lacZ (fragment)"
                               /product="LacZ-alpha fragment of?beta-galactosidase"
                               /label="lacZ-alpha"
                               /protein_id="
                               "
                               /translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF
                               ASWRNSEEARTDRPSQQLRSL"
               misc_feature    11027..11062
                               /label="MCS"
                               /label="multiple cloning site from pUC9"
               primer_bind     complement(11063..11079)
                               /label="M13 fwd"
                               /label="common sequencing primer, one of multiple similar 
                               variants"
          ORIGIN
                  1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
                 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
                121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
                181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
                241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
                301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
                361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
                421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
                481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
                541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
                601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
                661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
                721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
                781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
                841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
                901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
                961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
               1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
               1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
               1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
               1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
               1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
               1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
               1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
               1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
               1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
               1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
               1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
               1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
               1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
               1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
               1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
               1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
               1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
               2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
               2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
               2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
               2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
               2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
               2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
               2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
               2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
               2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
               2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
               2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
               2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
               2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
               2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
               2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
               2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
               3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
               3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
               3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
               3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
               3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
               3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
               3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
               3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
               3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
               3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
               3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
               3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
               3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
               3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
               3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
               3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
               3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
               4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
               4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
               4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
               4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
               4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
               4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
               4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
               4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
               4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
               4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
               4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
               4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
               4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
               4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
               4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
               4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
               4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
               5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
               5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
               5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
               5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
               5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
               5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
               5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
               5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
               5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
               5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
               5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
               5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
               5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
               5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
               5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
               5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
               6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
               6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
               6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
               6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
               6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
               6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
               6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
               6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
               6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
               6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
               6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
               6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
               6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
               6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
               6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
               6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
               6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
               7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
               7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
               7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
               7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
               7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
               7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
               7381 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat
               7441 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
               7501 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
               7561 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
               7621 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
               7681 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
               7741 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
               7801 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
               7861 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
               7921 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
               7981 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
               8041 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
               8101 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
               8161 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
               8221 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
               8281 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
               8341 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
               8401 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
               8461 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
               8521 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
               8581 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
               8641 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
               8701 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt
               8761 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
               8821 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat
               8881 ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga cagatccggt
               8941 cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
               9001 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
               9061 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
               9121 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
               9181 atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
               9241 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
               9301 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
               9361 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
               9421 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
               9481 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
               9541 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
               9601 tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg
               9661 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
               9721 ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
               9781 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
               9841 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
               9901 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
               9961 agttcaggct ttttcatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag
              10021 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta
              10081 tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
              10141 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
              10201 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
              10261 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
              10321 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
              10381 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
              10441 tagacgagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg
              10501 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
              10561 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
              10621 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
              10681 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
              10741 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggcc
              10801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
              10861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
              10921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
              10981 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggctgcag
              11041 gtcgacggat ccccgggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
              11101 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
              11161 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaag cctagggagg
              11221 agtccac
          //
          

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