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          pCambia1391

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          pCambia 1391載體基本信息

          出品公司: CAMBIA
          載體名稱: pCAMBIA1391
          質粒類型: 植物載體;農桿菌雙元表達載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: --
          載體大小: 10658 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: GUS、6xHis
          載體抗性: 卡那霉素
          篩選標記: Hygromycin
          克隆菌株: HB101等菌株
          宿主細胞(系): 植物細胞、農桿菌
          備注: --
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          pCambia 1391載體質粒圖譜和多克隆位點信息

          pCAMBIA1391載體圖譜



          pCAMBIA1391 多克隆位點

          pCambia 1391載體簡介

          pCambia 1391載體序列

          LOCUS       pCAMBIA1391          10658 bp ds-DNA    circular SYN 16-四月-2013
          DEFINITION  Agrobacterium binary vector for plant transformation, with 
                      hygromycin- and kanamycin-resistance genes and GUS lacking a start 
                      codon.
          ACCESSION   AF234308
          VERSION     .
          KEYWORDS    pCAMBIA1391
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          COMMENT     The GenBank record was corrected by inserting a G at position 4541.
          FEATURES             Location/Qualifiers
               source          1..10658
                               /organism="synthetic DNA construct"
                               /lab_host="Plant Cells"
                               /mol_type="other DNA"
               CDS             20..1825
                               /codon_start=1
                               /gene="uidA?(or gusA)?from E. coli"
                               /product="beta-glucuronidase"
                               /note="GUS"
               CDS             1832..1849
                               /codon_start=1
                               /product="6xHis affinity tag"
                               /note="6xHis"
                               /translation="HHHHHH"
               terminator      1881..2133
                               /note="NOS terminator"
                               /note="nopaline synthase terminator and poly(A) signal"
               misc_feature    2155..2179
                               /note="RB T-DNA repeat"
                               /note="right border repeat from nopaline C58 T-DNA"
               CDS             3479..4108
                               /codon_start=1
                               /product="stability protein from Pseudomonas plasmid pVS1"
                               /note="pVS1 StaA"
               CDS             4537..5610
                               /codon_start=1
                               /product="replication protein from Pseudomonas plasmid 
                               pVS1"
                               /note="pVS1 RepA"
                               /note="
                               "
                               /protein_id="
                               "
               misc_feature    6214..6354
                               /note="bom"
                               /note="basis of mobility region from pBR322"
               rep_origin      complement(6540..7128)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(7215..8009)
                               /codon_start=1
                               /gene="aphA-3"
                               /product="aminoglycoside phosphotransferase"
                               /note="KanR"
                               /note="confers resistance to kanamycin"
                               /protein_id="
                               "
               misc_feature    8434..8458
                               /note="LB T-DNA repeat"
                               /note="left border repeat from nopaline C58 T-DNA"
               polyA_signal    8536..8710
                               /note="CaMV poly(A) signal"
                               /note="cauliflower mosaic virus polyadenylation signal"
               CDS             complement(8750..9775)
                               /codon_start=1
                               /product="hygromycin B phosphotransferase"
                               /note="HygR"
                               /note="confers resistance to hygromycin"
               promoter        complement(9843..10520)
                               /note="CaMV 35S promoter (enhanced)"
                               /note="cauliflower mosaic virus 35S promoter with a 
                               duplicated enhancer region"
               misc_feature    10609..10644
                               /note="MCS"
                               /note="multiple cloning site from pUC9"
          ORIGIN
                  1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
                 61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
                121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
                181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
                241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
                301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
                361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
                421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
                481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
                541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
                601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
                661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
                721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
                781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
                841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
                901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
                961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
               1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
               1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
               1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
               1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
               1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
               1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
               1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
               1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
               1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
               1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
               1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
               1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
               1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
               1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg
               1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
               1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
               1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
               2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
               2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag
               2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
               2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
               2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
               2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
               2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
               2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
               2521 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
               2581 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
               2641 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
               2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
               2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
               2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
               2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
               2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
               3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
               3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
               3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
               3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
               3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
               3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
               3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
               3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
               3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
               3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
               3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
               3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
               3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
               3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
               3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
               3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
               3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
               4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
               4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
               4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
               4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
               4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
               4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
               4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
               4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
               4501 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
               4561 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
               4621 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
               4681 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
               4741 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
               4801 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
               4861 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
               4921 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
               4981 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
               5041 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
               5101 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
               5161 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
               5221 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
               5281 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
               5341 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
               5401 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
               5461 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
               5521 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
               5581 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
               5641 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
               5701 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
               5761 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
               5821 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
               5881 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
               5941 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
               6001 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
               6061 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
               6121 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
               6181 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
               6241 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
               6301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
               6361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
               6421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
               6481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
               6541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
               6601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
               6661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
               6721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
               6781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
               6841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
               6901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
               6961 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
               7021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
               7081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
               7141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
               7201 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
               7261 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
               7321 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
               7381 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
               7441 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
               7501 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
               7561 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
               7621 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
               7681 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
               7741 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
               7801 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
               7861 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
               7921 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
               7981 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
               8041 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
               8101 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
               8161 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
               8221 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
               8281 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
               8341 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
               8401 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
               8461 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
               8521 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
               8581 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
               8641 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
               8701 tatggagaaa ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg
               8761 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
               8821 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
               8881 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
               8941 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg
               9001 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
               9061 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
               9121 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
               9181 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
               9241 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
               9301 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
               9361 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
               9421 tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
               9481 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt
               9541 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
               9601 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
               9661 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
               9721 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc
               9781 attgcccccc gggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
               9841 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag
               9901 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
               9961 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
              10021 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
              10081 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
              10141 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
              10201 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
              10261 ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
              10321 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
              10381 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
              10441 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
              10501 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
              10561 tggagtagac gagagtgtcg tgctccacca tgttgggccc ggcgcgccaa gcttggctgc
              10621 aggtcgacgg atccccggga attctaagag gagtccac
          //
          

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