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          pCambia1201

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          pCambia 1201載體基本信息

          出品公司: CAMBIA
          載體名稱: pCAMBIA1201
          質粒類型: 植物載體;農桿菌雙元表達載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: lac
          載體大小: 12002 bp
          5' 測序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
          3' 測序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
          載體標簽: --
          載體抗性: 氯霉素
          篩選標記: Hygromycin、GUS
          克隆菌株: HB101等菌株
          宿主細胞(系): 植物細胞、農桿菌
          備注: --
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 誘導型
          病毒/非病毒: 非病毒

          pCambia 1201載體質粒圖譜和多克隆位點信息

          pCAMBIA1201載體圖譜



          pCAMBIA1201 多克隆位點

          pCambia 1201載體簡介

          pCambia 1201載體序列

          LOCUS       pCambia1201          12002 bp ds-DNA    circular SYN 17-五月-2013
          DEFINITION  Agrobacterium binary vector for plant transformation, with 
                      hygromycin- and chloramphenicol-resistance and GUS genes.
          ACCESSION   AF234293
          VERSION     .
          KEYWORDS    pCAMBIA1201
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          COMMENT     The GenBank record was corrected by inserting a G at position 4743.
          FEATURES             Location/Qualifiers
               source          1..12002
                               /organism="synthetic DNA construct"
                               /lab_host="Plant Cells"
                               /mol_type="other DNA"
               CDS             join(2..16,207..2024)
                               /codon_start=1
                               /product="beta-glucuronidase"
                               /label="GUS"
                               /label="This version of the gusA gene has a 5' extension 
                               with a catalase intron to ensure expression in plants but 
                               not bacteria."
                               /protein_id="
                               "
               intron          17..206
                               /label="cat1 intron"
                               /label="castor bean catalase intron, modified"
               CDS             2031..2048
                               /codon_start=1
                               /product="6xHis affinity tag"
                               /label="6xHis"
                               /translation="HHHHHH"
               terminator      2083..2335
                               /label="NOS terminator"
                               /label="nopaline synthase terminator and poly(A) signal"
               misc_feature    2357..2381
                               /label="RB T-DNA repeat"
                               /label="right border repeat from nopaline C58 T-DNA"
               CDS             3681..4310
                               /codon_start=1
                               /product="stability protein from Pseudomonas plasmid pVS1"
                               /label="pVS1 StaA"
               CDS             4739..5812
                               /codon_start=1
                               /product="replication protein from Pseudomonas plasmid 
                               pVS1"
                               /label="pVS1 RepA"
                               /label="
                               "
                               /protein_id="
                               "
               misc_feature    6416..6556
                               /label="bom"
                               /label="basis of mobility region from pBR322"
               rep_origin      complement(6742..7330)
                               /direction=LEFT
                               /label="ori"
                               /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(7601..8260)
                               /codon_start=1
                               /gene="cat"
                               /product="chloramphenicol acetyltransferase"
                               /label="CmR"
                               /label="confers resistance to chloramphenicol"
               misc_feature    8788..8812
                               /label="LB T-DNA repeat"
                               /label="left border repeat from nopaline C58 T-DNA"
               polyA_signal    8890..9064
                               /label="CaMV poly(A) signal"
                               /label="cauliflower mosaic virus polyadenylation signal"
               CDS             complement(9104..10129)
                               /codon_start=1
                               /product="hygromycin B phosphotransferase"
                               /label="HygR"
                               /label="confers resistance to hygromycin"
               promoter        complement(10197..10874)
                               /label="CaMV 35S promoter (enhanced)"
                               /label="cauliflower mosaic virus 35S promoter with a 
                               duplicated enhancer region"
               promoter        11101..11131
                               /label="lac promoter"
                               /label="promoter for the E. coli lac operon"
               protein_bind    11139..11155
                               /bound_moiety="lac repressor encoded by lacI"
                               /label="lac operator"
                               /label="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               primer_bind     11163..11179
                               /label="M13 rev"
                               /label="common sequencing primer, one of multiple similar 
                               variants"
               misc_feature    11189..11245
                               /gene="
                               "
                               /label="MCS"
                               /label="pUC18/19 multiple cloning site"
               primer_bind     complement(11249..11265)
                               /label="M13 fwd"
                               /label="common sequencing primer, one of multiple similar 
                               variants"
               promoter        11642..11987
                               /label="CaMV 35S promoter"
                               /label="strong constitutive promoter from cauliflower mosaic
                               virus"
          ORIGIN
                  1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
                 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
                121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
                181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
                241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
                301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
                361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
                421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
                481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
                541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
                601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
                661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
                721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
                781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
                841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
                901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
                961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
               1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
               1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
               1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
               1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
               1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
               1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
               1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
               1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
               1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
               1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
               1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
               1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
               1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
               1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
               1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
               1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
               1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
               2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
               2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
               2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
               2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
               2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
               2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
               2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
               2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
               2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
               2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
               2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
               2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
               2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
               2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
               2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
               2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
               3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
               3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
               3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
               3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
               3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
               3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
               3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
               3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
               3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
               3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
               3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
               3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
               3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
               3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
               3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
               3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
               3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
               4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
               4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
               4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
               4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
               4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
               4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
               4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
               4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
               4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
               4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
               4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
               4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
               4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
               4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
               4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
               4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
               4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
               5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
               5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
               5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
               5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
               5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
               5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
               5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
               5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
               5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
               5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
               5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
               5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
               5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
               5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
               5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
               5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
               6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
               6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
               6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
               6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
               6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
               6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
               6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
               6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
               6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
               6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
               6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
               6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
               6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
               6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
               6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
               6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
               6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
               7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
               7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
               7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
               7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
               7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
               7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
               7381 ctcacgttaa gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta
               7441 tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg
               7501 tgcttagtgc atctaatcgc ttgagttaac gccggcgaag cggcgtcggc ttgaacgaat
               7561 ttctagctag aggatcgcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
               7621 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
               7681 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
               7741 ccattgtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
               7801 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
               7861 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
               7921 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
               7981 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
               8041 ggaactccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
               8101 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
               8161 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
               8221 atatatcaac ggtggtatat ccagtgattt ttttctccat gatgtttaac tttgttttag
               8281 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
               8341 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca
               8401 taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
               8461 ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag
               8521 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt
               8581 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
               8641 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
               8701 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
               8761 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
               8821 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
               8881 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
               8941 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
               9001 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
               9061 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg
               9121 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
               9181 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
               9241 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
               9301 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg
               9361 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
               9421 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
               9481 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
               9541 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
               9601 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
               9661 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
               9721 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
               9781 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
               9841 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca
               9901 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
               9961 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
              10021 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
              10081 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
              10141 ccccgggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag
              10201 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt
              10261 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
              10321 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
              10381 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
              10441 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
              10501 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
              10561 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
              10621 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
              10681 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
              10741 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
              10801 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
              10861 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
              10921 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc
              10981 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
              11041 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
              11101 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
              11161 cacaggaaac agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag
              11221 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
              11281 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
              11341 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
              11401 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttagcttca
              11461 tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt
              11521 tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc
              11581 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
              11641 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
              11701 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
              11761 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
              11821 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
              11881 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
              11941 aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcttg
              12001 ac
          //
          

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