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          pShuttle-CMV

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Agilent
          載體名稱: pShuttle-CMV, pShuttle CMV
          質粒類型: 腺病毒表達載體,哺乳動物細胞表達載體
          高拷貝/低拷貝: 低拷貝
          啟動子: CMV
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 7469 bp
          5' 測序引物及序列: pShuttle-CMV-F: GGTCTATATAAGCAGAGCTG
          3' 測序引物及序列: pShuttle-CMV-R: GTGGTATGGCTGATTATGATCAG
          載體標簽: --
          載體抗性: 卡那
          備注: --
          產品目錄號: 240007
          穩定性: /
          組成型: --
          病毒/非病毒: 腺病毒

          載體質粒圖譜和多克隆位點信息

          pShuttle-CMV載體圖譜


          pShuttle-CMV多克隆位點

          pShuttle-CMV載體特征

          載體簡介

          The vector pShuttle-CMV contains a multiple cloning site sandwiched between the CMV promoter and the SV40 polyadenylation signal and is suitable for insertion of a large cDNA (up to 6.6 kb). pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.

          載體序列

          LOCUS       pShuttle-CMV	7469 bp 	DNA     circular	SYN
          DEFINITION  pShuttle CMV
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               source          1..7469
                               /organism="pShuttle CMV"
                               /mol_type="other DNA"
               misc_feature    complement(1..103)
                               /label="RITR"
               misc_feature    1..103
                               /label="LITR"
               misc_feature    183..331
                               /label="Encap"
               promoter        348..900
                               /label="CMV_immearly_promoter"
               misc_feature    403..690
                               /label="CAG_enhancer"
               misc_feature    857..877
                               /label="CMV_fwd_primer"
               promoter        858..927
                               /label="CMV_promoter"
               terminator      1010..1238
                               /label="SV40_PA_terminator"
               misc_feature    1208..1227
                               /label="EBV_rev_primer"
               CDS             complement(1814..3172)
                               /label="ORF frame 2"
               CDS             complement(2920..3441)
                               /label="ORF frame 3"
               misc_feature    complement(4429..4531)
                               /label="LITR"
               misc_feature    4429..4531
                               /label="RITR"
               rep_origin      complement(4776..5395)
                               /label="pBR322_origin"
               gene            complement(5550..5861)
                               /label="Ampicillin (311 - 0)"
                               /gene="Ampicillin (311 - 0)"
               promoter        6073..6122
                               /label="NEOKAN_promoter"
               CDS             6211..7005
                               /label="ORF frame 1"
               gene            6214..7002
                               /label="NeoR/KanR"
                               /gene="NeoR/KanR"
          ORIGIN
              1 CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG GGGGTGGAGT
             61 TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG TAGTAGTGTG GCGGAAGTGT
            121 GATGTTGCAA GTGTGGCGGA ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG
            181 GTGTGCGCCG GTGTACACAG GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG
            241 TAAATTTGGG CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
            301 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA GTAATCAATT
            361 ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT
            421 GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT
            481 CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA
            541 ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
            601 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT
            661 ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG
            721 TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT
            781 GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC
            841 AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
            901 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG GTACCGTCGA CGCGGCCGCT
            961 CGAGCCTAAG CTTCTAGATA AGATATCCGA TCCACCGGAT CTAGATAACT GATCATAATC
           1021 AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG
           1081 AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT
           1141 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT
           1201 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTAAC GCGGATCTGG GCGTGGTTAA
           1261 GGGTGGGAAA GAATATATAA GGTGGGGGTC TTATGTAGTT TTGTATCTGT TTTGCAGCAG
           1321 CCGCCGCCGC CATGAGCACC AACTCGTTTG ATGGAAGCAT TGTGAGCTCA TATTTGACAA
           1381 CGCGCATGCC CCCATGGGCC GGGGTGCGTC AGAATGTGAT GGGCTCCAGC ATTGATGGTC
           1441 GCCCCGTCCT GCCCGCAAAC TCTACTACCT TGACCTACGA GACCGTGTCT GGAACGCCGT
           1501 TGGAGACTGC AGCCTCCGCC GCCGCTTCAG CCGCTGCAGC CACCGCCCGC GGGATTGTGA
           1561 CTGACTTTGC TTTCCTGAGC CCGCTTGCAA GCAGTGCAGC TTCCCGTTCA TCCGCCCGCG
           1621 ATGACAAGTT GACGGCTCTT TTGGCACAAT TGGATTCTTT GACCCGGGAA CTTAATGTCG
           1681 TTTCTCAGCA GCTGTTGGAT CTGCGCCAGC AGGTTTCTGC CCTGAAGGCT TCCTCCCCTC
           1741 CCAATGCGGT TTAAAACATA AATAAAAAAC CAGACTCTGT TTGGATTTGG ATCAAGCAAG
           1801 TGTCTTGCTG TCTTTATTTA GGGGTTTTGC GCGCGCGGTA GGCCCGGGAC CAGCGGTCTC
           1861 GGTCGTTGAG GGTCCTGTGT ATTTTTTCCA GGACGTGGTA AAGGTGACTC TGGATGTTCA
           1921 GATACATGGG CATAAGCCCG TCTCTGGGGT GGAGGTAGCA CCACTGCAGA GCTTCATGCT
           1981 GCGGGGTGGT GTTGTAGATG ATCCAGTCGT AGCAGGAGCG CTGGGCGTGG TGCCTAAAAA
           2041 TGTCTTTCAG TAGCAAGCTG ATTGCCAGGG GCAGGCCCTT GGTGTAAGTG TTTACAAAGC
           2101 GGTTAAGCTG GGATGGGTGC ATACGTGGGG ATATGAGATG CATCTTGGAC TGTATTTTTA
           2161 GGTTGGCTAT GTTCCCAGCC ATATCCCTCC GGGGATTCAT GTTGTGCAGA ACCACCAGCA
           2221 CAGTGTATCC GGTGCACTTG GGAAATTTGT CATGTAGCTT AGAAGGAAAT GCGTGGAAGA
           2281 ACTTGGAGAC GCCCTTGTGA CCTCCAAGAT TTTCCATGCA TTCGTCCATA ATGATGGCAA
           2341 TGGGCCCACG GGCGGCGGCC TGGGCGAAGA TATTTCTGGG ATCACTAACG TCATAGTTGT
           2401 GTTCCAGGAT GAGATCGTCA TAGGCCATTT TTACAAAGCG CGGGCGGAGG GTGCCAGACT
           2461 GCGGTATAAT GGTTCCATCC GGCCCAGGGG CGTAGTTACC CTCACAGATT TGCATTTCCC
           2521 ACGCTTTGAG TTCAGATGGG GGGATCATGT CTACCTGCGG GGCGATGAAG AAAACGGTTT
           2581 CCGGGGTAGG GGAGATCAGC TGGGAAGAAA GCAGGTTCCT GAGCAGCTGC GACTTACCGC
           2641 AGCCGGTGGG CCCGTAAATC ACACCTATTA CCGGCTGCAA CTGGTAGTTA AGAGAGCTGC
           2701 AGCTGCCGTC ATCCCTGAGC AGGGGGGCCA CTTCGTTAAG CATGTCCCTG ACTCGCATGT
           2761 TTTCCCTGAC CAAATCCGCC AGAAGGCGCT CGCCGCCCAG CGATAGCAGT TCTTGCAAGG
           2821 AAGCAAAGTT TTTCAACGGT TTGAGACCGT CCGCCGTAGG CATGCTTTTG AGCGTTTGAC
           2881 CAAGCAGTTC CAGGCGGTCC CACAGCTCGG TCACCTGCTC TACGGCATCT CGATCCAGCA
           2941 TATCTCCTCG TTTCGCGGGT TGGGGCGGCT TTCGCTGTAC GGCAGTAGTC GGTGCTCGTC
           3001 CAGACGGGCC AGGGTCATGT CTTTCCACGG GCGCAGGGTC CTCGTCAGCG TAGTCTGGGT
           3061 CACGGTGAAG GGGTGCGCTC CGGGCTGCGC GCTGGCCAGG GTGCGCTTGA GGCTGGTCCT
           3121 GCTGGTGCTG AAGCGCTGCC GGTCTTCGCC CTGCGCGTCG GCCAGGTAGC ATTTGACCAT
           3181 GGTGTCATAG TCCAGCCCCT CCGCGGCGTG GCCCTTGGCG CGCAGCTTGC CCTTGGAGGA
           3241 GGCGCCGCAC GAGGGGCAGT GCAGACTTTT GAGGGCGTAG AGCTTGGGCG CGAGAAATAC
           3301 CGATTCCGGG GAGTAGGCAT CCGCGCCGCA GGCCCCGCAG ACGGTCTCGC ATTCCACGAG
           3361 CCAGGTGAGC TCTGGCCGTT CGGGGTCAAA AACCAGGTTT CCCCCATGCT TTTTGATGCG
           3421 TTTCTTACCT CTGGTTTCCA TGAGCCGGTG TCCACGCTCG GTGACGAAAA GGCTGTCCGT
           3481 GTCCCCGTAT ACAGACTTGA GAGGGAGTTT AAACGAATTC AATAGCTTGT TGCATGGGCG
           3541 GCGATATAAA ATGCAAGGTG CTGCTCAAAA AATCAGGCAA AGCCTCGCGC AAAAAAGAAA
           3601 GCACATCGTA GTCATGCTCA TGCAGATAAA GGCAGGTAAG CTCCGGAACC ACCACAGAAA
           3661 AAGACACCAT TTTTCTCTCA AACATGTCTG CGGGTTTCTG CATAAACACA AAATAAAATA
           3721 ACAAAAAAAC ATTTAAACAT TAGAAGCCTG TCTTACAACA GGAAAAACAA CCCTTATAAG
           3781 CATAAGACGG ACTACGGCCA TGCCGGCGTG ACCGTAAAAA AACTGGTCAC CGTGATTAAA
           3841 AAGCACCACC GACAGCTCCT CGGTCATGTC CGGAGTCATA ATGTAAGACT CGGTAAACAC
           3901 ATCAGGTTGA TTCACATCGG TCAGTGCTAA AAAGCGACCG AAATAGCCCG GGGGAATACA
           3961 TACCCGCAGG CGTAGAGACA ACATTACAGC CCCCATAGGA GGTATAACAA AATTAATAGG
           4021 AGAGAAAAAC ACATAAACAC CTGAAAAACC CTCCTGCCTA GGCAAAATAG CACCCTCCCG
           4081 CTCCAGAACA ACATACAGCG CTTCCACAGC GGCAGCCATA ACAGTCAGCC TTACCAGTAA
           4141 AAAAGAAAAC CTATTAAAAA AACACCACTC GACACGGCAC CAGCTCAATC AGTCACAGTG
           4201 TAAAAAAGGG CCAAGTGCAG AGCGAGTATA TATAGGACTA AAAAATGACG TAACGGTTAA
           4261 AGTCCACAAA AAACACCCAG AAAACCGCAC GCGAACCTAC GCCCAGAAAC GAAAGCCAAA
           4321 AAACCCACAA CTTCCTCAAA TCGTCACTTC CGTTTTCCCA CGTTACGTCA CTTCCCATTT
           4381 TAAGAAAACT ACAATTCCCA ACACATACAA GTTACTCCGC CCTAAAACCT ACGTCACCCG
           4441 CCCCGTTCCC ACGCCCCGCG CCACGTCACA AACTCCACCC CCTCATTATC ATATTGGCTT
           4501 CAATCCAAAA TAAGGTATAT TATTGATGAT GTTAATTAAC ATGCATGGAT CCATATGCGG
           4561 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC
           4621 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
           4681 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
           4741 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
           4801 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
           4861 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
           4921 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
           4981 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
           5041 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
           5101 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
           5161 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
           5221 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
           5281 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
           5341 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
           5401 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
           5461 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
           5521 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
           5581 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
           5641 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC
           5701 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
           5761 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
           5821 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGC CATGAGATTA
           5881 TCAAAAAGGA TCTTCACCTA GATCCTTTTC ACGTAGAAAG CCAGTCCGCA GAAACGGTGC
           5941 TGACCCCGGA TGAATGTCAG CTACTGGGCT ATCTGGACAA GGGAAAACGC AAGCGCAAAG
           6001 AGAAAGCAGG TAGCTTGCAG TGGGCTTACA TGGCGATAGC TAGACTGGGC GGTTTTATGG
           6061 ACAGCAAGCG AACCGGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC
           6121 AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGCTCT
           6181 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
           6241 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
           6301 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
           6361 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG
           6421 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
           6481 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
           6541 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
           6601 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
           6661 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
           6721 TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT
           6781 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
           6841 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
           6901 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
           6961 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAATTTT GTTAAAATTT
           7021 TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCACCATCC CTTATAAATC
           7081 AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT
           7141 AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT
           7201 ACGTGAACCA TCACCCTAAT CAAGTTTTTT GTGGTCGAGG TGCCGTAAAG CACTAAATCG
           7261 GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG
           7321 AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC
           7381 GCTGCGCGTA ACCACCACAC CCGCGCGCTT AATGCGCCGC TACAGGGCGC GTCCATTCGC
           7441 CATTCAGGAT CGAATTAATT CTTAATTAA
          //
          

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