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          pDC411

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Microbix Biosystems
          載體名稱: pDC411
          質粒類型: 腺病毒表達載體
          高拷貝/低拷貝: --
          啟動子: --
          克隆方法: --
          載體大小: 5574 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素(Ampicillin)
          篩選標記: --
          備注: 克隆宿主菌:DB3.1
          產品目錄號: --
          穩定性: --
          組成型: 組成型
          病毒/非病毒: 腺病毒

          載體質粒圖譜和多克隆位點信息

          pDC411載體圖譜



          載體簡介

          載體序列

          LOCUS       pDC411       5574 bp dsDNA    Circular
          DEFINITION  Puc based shuttle plasmid  for Ad vector rescue via homologous recomb. Ng et al. Hum. Gene Ther. 11: xxx, 2000.
          COMMENT     This file is best viewed with a free Vector NTI Viewer:
          COMMENT     http://www.informaxinc.com/view/viewer.html
          COMMENT     VNTREPLTYPE|Plasmid|
          COMMENT     VNTEXTCHREPL|Bacteria|
          FEATURES               Location/Qualifiers
               misc_feature    515..2785
                               /note="Main body of the Ad5 sequences present in this vector system with E1 deleted but with a normal packaging signal."
                               /label=Ad\5\E1\deleted
               CDS             600..1019
                               /note="Protein IX open reading frame only, does NOT include promoter/ terminator etc."
                               /label=pIX
               rep_origin      3264..3564
                               /label=ori
               misc_feature    5115..453
                               /label=Ad
               CDS             complement(4014..4830)
                               /label=amp
               CDS             5484..1
                               /label=ITR
               CDS             complement(2..102)
                               /label=ITR
          ORIGIN
                  1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 
                 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 
                121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 
                181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 
                241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 
                301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 
                361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 
                421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gaattcaggc ctgctagcag 
                481 atctaagctt gagctcgtcg actcgaagat cgatctgggc gtggttaagg gtgggaaaga 
                541 atatataagg tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca 
                601 tgagcaccaa ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc 
                661 catgggccgg ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc 
                721 ccgcaaactc tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag 
                781 cctccgccgc cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt 
                841 tcctgagccc gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga 
                901 cggctctttt ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc 
                961 tgttggatct gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt 
               1021 aaaacataaa taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc 
               1081 tttatttagg ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg 
               1141 tcctgtgtat tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca 
               1201 taagcccgtc tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt 
               1261 tgtagatgat ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta 
               1321 gcaagctgat tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg 
               1381 atgggtgcat acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt 
               1441 tcccagccat atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg 
               1501 tgcacttggg aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc 
               1561 ccttgtgacc tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg 
               1621 cggcggcctg ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga 
               1681 gatcgtcata ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg 
               1741 ttccatccgg cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt 
               1801 cagatggggg gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg 
               1861 agatcagctg ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc 
               1921 cgtaaatcac acctattacc gggtgcaact ggtagttaag agagctgcag ctgccgtcat 
               1981 ccctgagcag gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca 
               2041 aatccgccag aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt 
               2101 tcaacggttt gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca 
               2161 ggcggtccca cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt 
               2221 tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag 
               2281 ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg 
               2341 gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa 
               2401 gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc 
               2461 cagcccctcc gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga 
               2521 ggggcagtgc agacttttga gggcgtagag cttgggcgcg agaaataccg attccgggga 
               2581 gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc 
               2641 tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct 
               2701 ggtttccatg agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac 
               2761 agacttgaga ggcctgtcct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 
               2821 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 
               2881 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 
               2941 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 
               3001 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 
               3061 gcatggcggc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 
               3121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
               3181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
               3241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
               3301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
               3361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
               3421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
               3481 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg 
               3541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
               3601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
               3661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
               3721 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
               3781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
               3841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
               3901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
               3961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
               4021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
               4081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
               4141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
               4201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
               4261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
               4321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
               4381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
               4441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
               4501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
               4561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
               4621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
               4681 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
               4741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
               4801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
               4861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
               4921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
               4981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
               5041 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 
               5101 ataggcgtat cactctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt 
               5161 cacagcggca gcctaacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac 
               5221 cactcgacac ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga 
               5281 gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac 
               5341 cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc 
               5401 acttccgttt tcccacgtta cgtaacttcc cattttaaga aaactacaat tcccaacaca 
               5461 tacaagttac tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg 
               5521 tcacaaactc caccccctca ttatcatatt ggcttcaatc caaaataagg tata 
          //
          

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