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          pAdTrack

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Agilent
          載體名稱: pAdTrack
          質粒類型: 哺乳動物細胞腺病毒表達載體
          高拷貝/低拷貝: 低拷貝
          啟動子: --
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 8175 bp
          5' 測序引物及序列: /
          3' 測序引物及序列: /
          載體標簽: EGFP
          載體抗性: 卡那
          備注: pAdTrack載體用來轉基因腺病毒細胞表達,載體擁有一個EGFP綠色熒光蛋白跟蹤基因表達結果,和pAdeasy1載體共同使用時最大能夠插入5.9kb,pAdeasy2載體共同使用時能夠最大插入8.6kb片段。具體使用方法可以參考pAdeasy載體系統。
          產品目錄號: 240005
          穩定性: /
          組成型: --
          病毒/非病毒: 腺病毒

          載體質粒圖譜和多克隆位點信息

          pAdTrack載體圖譜


          載體簡介

          載體序列

          LOCUS       pAdTrack	8175 bp 	DNA     circular	SYN
          DEFINITION  pAdTrack
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               source          1..8175
                               /organism="pAdTrack"
                               /mol_type="other DNA"
               misc_feature    197..345
                               /label="Encap"
               misc_feature    complement(374..393)
                               /label="EBV_rev_primer"
               CDS             575..1354
                               /label="ORF frame 2"
               CDS             complement(593..1339)
                               /label="ORF frame 3"
               gene            complement(623..1336)
                               /label="EGFP"
                               /gene="EGFP"
               misc_feature    complement(665..686)
                               /label="EGFP_C_primer"
               misc_feature    complement(1127..1156)
                               /label="Y66 (EGFP)"
               misc_feature    1273..1294
                               /label="EGFP_N_primer"
               misc_feature    complement(1413..1433)
                               /label="CMV_fwd_primer"
               CDS             complement(2563..3921)
                               /label="ORF frame 1"
               CDS             complement(3669..4190)
                               /label="ORF frame 2"
               misc_feature    complement(5147..5242)
                               /label="LITR"
               misc_feature    5147..5242
                               /label="RITR"
               rep_origin      complement(5487..6106)
                               /label="pBR322_origin"
               gene            complement(6261..6560)
                               /label="Ampicillin (299 - 0)"
                               /gene="Ampicillin (299 - 0)"
               promoter        6765..6814
                               /label="NEOKAN_promoter"
               CDS             6903..7697
                               /label="ORF frame 3"
               gene            6906..7694
                               /label="NeoR/KanR"
                               /gene="NeoR/KanR"
          ORIGIN
              1 NNNTTAATTA ANNNTCCCTT CCAGCTCTCT GCCCCTTTTG GATTGAAGCC AATATGATAA
             61 TGAGGGGGTG GAGTTTGTGA CGTGGCGCGG GGCGTGGGAA CGGGGCGGGT GACGTAGTAG
            121 TGTGGCGGAA GTGTGATGTT GCAAGTGTGG CGGAACACAT GTAAGCGACG GATGTGGCAA
            181 AAGTGACGTT TTTGGTGTGC GCCGGTGTAC ACAGGAAGTG ACAATTTTCG CGCGGTTTTA
            241 GGCGGATGTT GTAGTAAATT TGGGCGTAAC CGAGTAAGAT TTGGCCATTT TCGCGGGAAA
            301 ACTGAATAAG AGGAAGTGAA ATCTGAATAA TTTTGTGTTA CTCATAGCGC GTAANNNCGC
            361 GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT
            421 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA
            481 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT
            541 TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC AGTTATCTAG
            601 ATCCGGTGGA TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
            661 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
            721 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
            781 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT
            841 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT
            901 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
            961 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
           1021 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
           1081 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT
           1141 AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA
           1201 ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT
           1261 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
           1321 CCTCGCCCTT GCTCACCATG GTGGCGACCG GTAGCGCTAG CGGATCTGAC GGTTCACTAA
           1381 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
           1441 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
           1501 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
           1561 ATTGATGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
           1621 CTGCCAAGTA GGAAAGTCCC ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
           1681 ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA
           1741 GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT
           1801 TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC
           1861 AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA
           1921 TGACCCCGTA ATTGATTACT ATTANNNCTA GCAGATCTGG TACCGTCGAC GCGGCCGCGA
           1981 TATCCTCGAG AAGCTTTCTA GAGNNNTAAG GGTGGGAAAG AATATATAAG GTGGGGGTCT
           2041 TATGTAGTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA ACTCGTTTGA
           2101 TGGAAGCATT GTGAGCTCAT ATTTGACAAC GCGCATGCCC CCATGGGCCG GGGTGCGTCA
           2161 GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG CCCGCAAACT CTACTACCTT
           2221 GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA GCCTCCGCCG CCGCTTCAGC
           2281 CGCTGCAGCC ACCGCCCGCG GGATTGTGAC TGACTTTGCT TTCCTGAGCC CGCTTGCAAG
           2341 CAGTGCAGCT TCCCGTTCAT CCGCCCGCGA TGACAAGTTG ACGGCTCTTT TGGCACAATT
           2401 GGATTCTTTG ACCCGGGAAC TTAATGTCGT TTCTCAGCAG CTGTTGGATC TGCGCCAGCA
           2461 GGTTTCTGCC CTGAAGGCTT CCTCCCCTCC CAATGCGGTT TAAAACATAA ATAAAAAACC
           2521 AGACTCTGTT TGGATTTGGA TCAAGCAAGT GTCTTGCTGT CTTTATTTAG GGGTTTTGCG
           2581 CGCGCGGTAG GCCCGGGACC AGCGGTCTCG GTCGTTGAGG GTCCTGTGTA TTTTTTCCAG
           2641 GACGTGGTAA AGGTGACTCT GGATGTTCAG ATACATGGGC ATAAGCCCGT CTCTGGGGTG
           2701 GAGGTAGCAC CACTGCAGAG CTTCATGCTG CGGGGTGGTG TTGTAGATGA TCCAGTCGTA
           2761 GCAGGAGCGC TGGGCGTGGT GCCTAAAAAT GTCTTTCAGT AGCAAGCTGA TTGCCAGGGG
           2821 CAGGCCCTTG GTGTAAGTGT TTACAAAGCG GTTAAGCTGG GATGGGTGCA TACGTGGGGA
           2881 TATGAGATGC ATCTTGGACT GTATTTTTAG GTTGGCTATG TTCCCAGCCA TATCCCTCCG
           2941 GGGATTCATG TTGTGCAGAA CCACCAGCAC AGTGTATCCG GTGCACTTGG GAAATTTGTC
           3001 ATGTAGCTTA GAAGGAAATG CGTGGAAGAA CTTGGAGACG CCCTTGTGAC CTCCAAGATT
           3061 TTCCATGCAT TCGTCCATAA TGATGGCAAT GGGCCCACGG GCGGCGGCCT GGGCGAAGAT
           3121 ATTTCTGGGA TCACTAACGT CATAGTTGTG TTCCAGGATG AGATCGTCAT AGGCCATTTT
           3181 TACAAAGCGC GGGCGGAGGG TGCCAGACTG CGGTATAATG GTTCCATCCG GCCCAGGGGC
           3241 GTAGTTACCC TCACAGATTT GCATTTCCCA CGCTTTGAGT TCAGATGGGG GGATCATGTC
           3301 TACCTGCGGG GCGATGAAGA AAACGGTTTC CGGGGTAGGG GAGATCAGCT GGGAAGAAAG
           3361 CAGGTTCCTG AGCAGCTGCG ACTTACCGCA GCCGGTGGGC CCGTAAATCA CACCTATTAC
           3421 CGGGTGCAAC TGGTAGTTAA GAGAGCTGCA GCTGCCGTCA TCCCTGAGCA GGGGGGCCAC
           3481 TTCGTTAAGC ATGTCCCTGA CTCGCATGTT TTCCCTGACC AAATCCGCCA GAAGGCGCTC
           3541 GCCGCCCAGC GATAGCAGTT CTTGCAAGGA AGCAAAGTTT TTCAACGGTT TGAGACCGTC
           3601 CGCCGTAGGC ATGCTTTTGA GCGTTTGACC AAGCAGTTCC AGGCGGTCCC ACAGCTCGGT
           3661 CACCTGCTCT ACGGCATCTC GATCCAGCAT ATCTCCTCGT TTCGCGGGTT GGGGCGGCTT
           3721 TCGCTGTACG GCAGTAGTCG GTGCTCGTCC AGACGGGCCA GGGTCATGTC TTTCCACGGG
           3781 CGCAGGGTCC TCGTCAGCGT AGTCTGGGTC ACGGTGAAGG GGTGCGCTCC GGGCTGCGCG
           3841 CTGGCCAGGG TGCGCTTGAG GCTGGTCCTG CTGGTGCTGA AGCGCTGCCG GTCTTCGCCC
           3901 TGCGCGTCGG CCAGGTAGCA TTTGACCATG GTGTCATAGT CCAGCCCCTC CGCGGCGTGG
           3961 CCCTTGGCGC GCAGCTTGCC CTTGGAGGAG GCGCCGCACG AGGGGCAGTG CAGACTTTTG
           4021 AGGGCGTAGA GCTTGGGCGC GAGAAATACC GATTCCGGGG AGTAGGCATC CGCGCCGCAG
           4081 GCCCCGCAGA CGGTCTCGCA TTCCACGAGC CAGGTGAGCT CTGGCCGTTC GGGGTCAAAA
           4141 ACCAGGTTTC CCCCATGCTT TTTGATGCGT TTCTTACCTC TGGTTTCCAT GAGCCGGTGT
           4201 CCACGCTCGG TGACGAAAAG GCTGTCCGTG TCCCCGTATA CAGACTNNNG TTTAAACGAA
           4261 TTCNNNTATA AAATGCAAGG TGCTGCTCAA AAAATCAGGC AAAGCCTCGC GCAAAAAAGA
           4321 AAGCACATCG TAGTCATGCT CATGCAGATA AAGGCAGGTA AGCTCCGGAA CCACCACAGA
           4381 AAAAGACACC ATTTTTCTCT CAAACATGTC TGCGGGTTTC TGCATAAACA CAAAATAAAA
           4441 TAACAAAAAA ACATTTAAAC ATTAGAAGCC TGTCTTACAA CAGGAAAAAC AACCCTTATA
           4501 AGCATAAGAC GGACTACGGC CATGCCGGCG TGACCGTAAA AAAACTGGTC ACCGTGATTA
           4561 AAAAGCACCA CCGACAGCTC CTCGGTCATG TCCGGAGTCA TAATGTAAGA CTCGGTAAAC
           4621 ACATCAGGTT GATTCATCGG TCAGTGCTAA AAAGCGACCG AAATAGCCCG GGGGAATACA
           4681 TACCCGCAGG CGTAGAGACA ACATTACAGC CCCCATAGGA GGTATAACAA AATTAATAGG
           4741 AGAGAAAAAC ACATAAACAC CTGAAAAACC CTCCTGCCTA GGCAAAATAG CACCCTCCCG
           4801 CTCCAGAACA ACATACAGCG CTTCACAGCG GCAGCCTAAC AGTCAGCCTT ACCAGTAAAA
           4861 AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA GCTCAATCAG TCACAGTGTA
           4921 AAAAAGGGCC AAGTGCAGAG CGAGTATATA TAGGACTAAA AAATGACGTA ACGGTTAAAG
           4981 TCCACAAAAA ACACCCAGAA AACCGCACGC GAACCTACGC CCAGAAACGA AAGCCAAAAA
           5041 ACCCACAACT TCCTCAAATC GTCACTTCCG TTTTCCCACG TTACGTAACT TCCCATTTTA
           5101 AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC TAAAACCTAC GTCACCCGCC
           5161 CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC TCATTATCAT ATTGGCTTCA
           5221 ATCCAAAATA AGGTATATTA TTGATGATNN NTTAATTAAG GATCCNNNCG GTGTGAAATA
           5281 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT
           5341 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
           5401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
           5461 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
           5521 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
           5581 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
           5641 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
           5701 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
           5761 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
           5821 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
           5881 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
           5941 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
           6001 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
           6061 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
           6121 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
           6181 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
           6241 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
           6301 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
           6361 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
           6421 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
           6481 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
           6541 CCAGTTAATA GTTTGCGCAA CGTTGTTGNN NNNNAAAAAG GATCTTCACC TAGATCCTTT
           6601 TCACGTAGAA AGCCAGTCCG CAGAAACGGT GCTGACCCCG GATGAATGTC AGCTACTGGG
           6661 CTATCTGGAC AAGGGAAAAC GCAAGCGCAA AGAGAAAGCA GGTAGCTTGC AGTGGGCTTA
           6721 CATGGCGATA GCTAGACTGG GCGGTTTTAT GGACAGCAAG CGAACCGGAA TTGCCAGCTG
           6781 GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT TTCTCGCCGC
           6841 CAAGGATCTG ATGGCGCAGG GGATCAAGCT CTGATCAAGA GACAGGATGA GGATCGTTTC
           6901 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
           6961 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
           7021 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
           7081 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
           7141 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
           7201 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
           7261 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
           7321 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
           7381 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
           7441 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
           7501 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
           7561 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
           7621 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
           7681 TTGACGAGTT CTTCTGAATT TTGTTAAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA
           7741 TAGGCCGAAA TCGGCAACAT CCCTTATAAA TCAAAAGAAT AGACCGCGAT AGGGTTGAGT
           7801 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG
           7861 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCAA ATCAAGTTTT
           7921 TTGCGGTCGA GGTGCCGTAA AGCTCTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA
           7981 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG
           8041 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCGCGC
           8101 TTAATGCGCC GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
           8161 NNNNNNNNNN NNNNN
          //
          

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                  免费高清视频 成人免费视频 成年在线视频免费观看视频