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          pAAV-RC

          價格:800元

          聯系方式:I47-825O-882O

          買家導航

          pAAVRC載體基本信息

          出品公司: Agilent
          載體名稱: pAAVRC, pAAV-RC, pAAV RC
          質粒類型: 腺病毒相關載體
          表達水平:
          啟動子: CMV
          載體大小: 7327 bp
          GenBank序列號: AF369963
          載體標簽: --
          載體抗性: 氨芐
          備注: --
          產品目錄號: 240071
          穩定性: /
          組成型: --
          病毒/非病毒: 腺病毒

          pAAVRC載體質粒圖譜和多克隆位點信息

          pAAV-RC載體圖譜

          pAAV-RC載體特征

          pAAVRC載體簡介

          pAAVRC載體序列

          LOCUS       pAAV-RC                7327 bp    DNA     circular SYN 22-JUL-2001
          DEFINITION  Cloning vector pAAV-RC, complete sequence.
          ACCESSION   AF369963
          VERSION     AF369963.1  GI:14994089
          KEYWORDS    .
          SOURCE      Cloning vector pAAV-RC
            ORGANISM  Cloning vector pAAV-RC
                      other sequences; artificial sequences; vectors.
          REFERENCE   1  (bases 1 to 7327)
            AUTHORS   Grafsky,A.J. III.
            TITLE     pAAV-RC for AAV Helper-Free System
            JOURNAL   Unpublished
          REFERENCE   2  (bases 1 to 7327)
            AUTHORS   Grafsky,A.J. III.
            TITLE     Direct Submission
            JOURNAL   Submitted (16-APR-2001) Technical Services, Stratagene, 11011 N.
                      Torrey Pines Rd., La Jolla, CA 92037, USA
          FEATURES             Location/Qualifiers
               source          1..7327
                               /organism="Cloning vector pAAV-RC"
                               /mol_type="genomic DNA"
                               /db_xref="taxon:161364"
                               /lab_host="Escherichia coli"
                               /note="used for recombinant DNA processing"
               CDS             131..1996
                               /note="AAV-2 rep"
                               /codon_start=1
                               /transl_table=11
                               /product="adeno-associated virus 2 rep protein"
                               /protein_id="AAK76418.1"
                               /db_xref="GI:14994090"
               CDS             2013..4220
                               /note="cap VP1"
                               /codon_start=1
                               /transl_table=11
                               /product="capsid protein VP1"
                               /protein_id="AAK76419.1"
                               /db_xref="GI:14994091"
               rep_origin      4705..5161
                               /note="f1 origin"
               CDS             5292..6152
                               /note="confers ampicillin resistance"
                               /codon_start=1
                               /transl_table=11
                               /product="beta-lactamase"
                               /protein_id="AAK76420.1"
                               /db_xref="GI:14994092"
               rep_origin      6161..7019
                               /note="ColE1 origin"
          ORIGIN      
                  1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa
                 61 gatcagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg
                121 cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga
                181 gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt
                241 gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga
                301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct
                361 tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac
                421 caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat
                481 tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac
                541 cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt
                601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag
                661 cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc
                721 gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag
                781 atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac
                841 ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc
                901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac
                961 taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg
               1021 gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct
               1081 gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac
               1141 taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt
               1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg
               1261 ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag
               1321 caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat
               1381 cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca
               1441 ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga
               1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt
               1561 ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc
               1621 cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac
               1681 gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca
               1741 cgtgggcatg aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc
               1801 aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc
               1861 tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat
               1921 gggaaaggtg ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg
               1981 catctttgaa caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt
               2041 ggctcgagga cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac
               2101 caccaccaaa gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt
               2161 acaagtacct cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg
               2221 ccgcggccct cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt
               2281 acctcaagta caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt
               2341 ttgggggcaa cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg
               2401 gcctggttga ggaacctgtt aagacggctc cgggaaaaaa gaggccggta gagcactctc
               2461 ctgtggagcc agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa
               2521 gattgaattt tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac
               2581 agccaccagc agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac
               2641 caatggcaga caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt
               2701 gcgattccac atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc
               2761 ccacctacaa caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca
               2821 atcactactt tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc
               2881 acttttcacc acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga
               2941 gactcaactt caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga
               3001 cgacgattgc caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc
               3061 tcccgtacgt cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct
               3121 tcatggtgcc acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct
               3181 cttcatttta ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta
               3241 ccttcagcta cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc
               3301 tggaccgtct catgaatcct ctcatcgacc agtacctgta ttacttgagc agaacaaaca
               3361 ctccaagtgg aaccaccacg cagtcaaggc ttcagttttc tcaggccgga gcgagtgaca
               3421 ttcgggacca gtctaggaac tggcttcctg gaccctgtta ccgccagcag cgagtatcaa
               3481 agacatctgc ggataacaac aacagtgaat actcgtggac tggagctacc aagtaccacc
               3541 tcaatggcag agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg
               3601 aagaaaagtt ttttcctcag agcggggttc tcatctttgg gaagcaaggc tcagagaaaa
               3661 caaatgtgga cattgaaaag gtcatgatta cagacgaaga ggaaatcagg acaaccaatc
               3721 ccgtggctac ggagcagtat ggttctgtat ctaccaacct ccagagaggc aacagacaag
               3781 cagctaccgc agatgtcaac acacaaggcg ttcttccagg catggtctgg caggacagag
               3841 atgtgtacct tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc
               3901 cctctcccct catgggtgga ttcggactta aacaccctcc tccacagatt ctcatcaaga
               3961 acaccccggt acctgcgaat ccttcgacca ccttcagtgc ggcaaagttt gcttccttca
               4021 tcacacagta ctccacggga caggtcagcg tggagatcga gtgggagctg cagaaggaaa
               4081 acagcaaacg ctggaatccc gaaattcagt acacttccaa ctacaacaag tctgttaatg
               4141 tggactttac tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat
               4201 acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc gtttcagttg
               4261 aactttggtc tctgcgtatt tctttcttat ctagtttcca tggctacgta gataagtagc
               4321 atggcgggtt aatcattaac tacagcccgg gcgtttaaac agcgggcgga ggggtggagt
               4381 cgtgacgtga attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt
               4441 ttgcgacatt ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca
               4501 gggtctccat tttgaagcgg gaggtttgaa cgagcgctgg cgcgctcact ggccgtcgtt
               4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
               4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
               4681 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
               4741 taaatttttg ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct
               4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
               4861 ccactattaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
               4921 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
               4981 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
               5041 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
               5101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
               5161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
               5221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
               5281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
               5341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
               5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
               5461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
               5521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
               5581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
               5641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
               5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
               5761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
               5821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
               5881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
               5941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
               6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
               6061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
               6121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
               6181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
               6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
               6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
               6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
               6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
               6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
               6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
               6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
               6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
               6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
               6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
               6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
               6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
               6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
               7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
               7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
               7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
               7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
               7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
               7321 acgccaa
          //
          

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