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          pAAV-CaMKIIa-EGFP

          價格:1100元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: 愛迪基因
          載體名稱: pAAV-CaMKIIa-EGFP
          質粒類型: 腺病毒載體;熒光報告載體
          高拷貝/低拷貝: 低拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: CaMKIIa
          載體大小: 6092 bp
          5' 測序引物及序列: ATGCTGACGAAGGCTCGCGA
          3' 測序引物及序列: GCATTAAAGCAGCGTATCCACATAGC
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: EGFP
          克隆菌株: Stbl3 或 NEB Stble
          宿主細胞(系): 神經細胞
          備注: --
          產品目錄號: # 50469
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 腺病毒

          載體質粒圖譜和多克隆位點信息

          pAAV-CaMKIIa-EGFP載體圖譜



          載體簡介

          載體序列

          LOCUS       pAAV-CaMKIIa-EGFP	6092 bp 	DNA	SYN
          DEFINITION  pAAV-CaMKIIa-EGFP
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     ORIGDB|GenBank
          COMMENT     VNTAUTHORNAME|biofeng.com|
          COMMENT     VNTNAME|pAAV-CaMKIIa-EGFP|     
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..6092
                               /organism="pAAV-CaMKIIa-EGFP"
                               /mol_type="other DNA"
               CDS             1475..2194
                               /label="ORF frame 2"
                               /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                               DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MVLLEFVTAAGITLGMDELYK*"
               gene            1478..2191
                               /label="EGFP"
                               /gene="EGFP"
                               /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                               DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MVLLEFVTAAGITLGMDELYK*"
               misc_feature    complement(1520..1541)
                               /label="EGFP_N_primer"
                               /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                               DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MVLLEFVTAAGITLGMDELYK*"
               misc_feature    1658..1687
                               /label="Y66 (EGFP)"
                               /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                               DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MVLLEFVTAAGITLGMDELYK*"
               misc_feature    2128..2149
                               /label="EGFP_C_primer"
                               /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                               DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MVLLEFVTAAGITLGMDELYK*"
               misc_feature    2221..2808
                               /label="WPRE"
                               /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                               DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MVLLEFVTAAGITLGMDELYK*"
               CDS             2309..2920
                               /label="ORF frame 2"
                               /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                               ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                               SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                               LWAASPHRYRALLERSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
               CDS             2322..2855
                               /label="ORF frame 3"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTERCSRDLRVASL*"
               terminator      2837..3315
                               /label="hGH_PA_terminator"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTERCSRDLRVASL*"
               rep_origin      3587..3893
                               /label="f1_origin"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTERCSRDLRVASL*"
               misc_feature    4161..4183
                               /label="pGEX_3_primer"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTERCSRDLRVASL*"
               promoter        4342..4370
                               /label="AmpR_promoter"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTERCSRDLRVASL*"
               gene            4412..5272
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTERCSRDLRVASL*"
               CDS             4412..5272
                               /label="ORF frame 2"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               rep_origin      5427..6046
                               /label="pBR322_origin"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
             61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
            121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
            181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
            241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
            301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
            361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
            421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
            481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
            541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
            601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
            661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
            721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
            781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
            841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
            901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
            961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
           1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
           1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
           1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
           1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
           1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
           1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
           1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
           1441 ggggcagctc tagagcggta ccggatccgc caccatggtg agcaagggcg aggagctgtt
           1501 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
           1561 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
           1621 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
           1681 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
           1741 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
           1801 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
           1861 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
           1921 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
           1981 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
           2041 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
           2101 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
           2161 gatcactctc ggcatggacg agctgtacaa gtaggaattc gatatcaagc ttatcgataa
           2221 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
           2281 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
           2341 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
           2401 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
           2461 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
           2521 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
           2581 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
           2641 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
           2701 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
           2761 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
           2821 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
           2881 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
           2941 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
           3001 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
           3061 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
           3121 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
           3181 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
           3241 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
           3301 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
           3361 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
           3421 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
           3481 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
           3541 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
           3601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
           3661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
           3721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
           3781 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
           3841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
           3901 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
           3961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
           4021 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
           4081 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
           4141 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
           4201 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
           4261 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
           4321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
           4381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
           4441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
           4501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
           4561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
           4621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
           4681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
           4741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
           4801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
           4861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
           4921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
           4981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
           5041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
           5101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
           5161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
           5221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
           5281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
           5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
           5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
           5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
           5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
           5581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
           5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
           5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
           5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
           5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
           5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
           5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
           6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
           6061 cctggccttt tgctggcctt ttgctcacat gt
          //
          
          

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