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          pAAV-CAG-RFP

          價格:1100元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: 愛迪基因
          載體名稱: pAAV-CAG-RFP
          質粒類型: 腺病毒載體;熒光蛋白報告載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: CAG (CMV enhancer/chicken β-actin promoter)
          載體大小: 7218 bp
          5' 測序引物及序列: pCAG fwd primer 5’CAACGTGCTGGTTATTGTG 3’
          3' 測序引物及序列: SP6 fwd primer 5’ATTTAGGTGACACTATAG 3’
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: DsRedExpress
          克隆菌株: Stbl3 或 NEB Stble
          宿主細胞(系): 常規哺乳動物細胞系
          備注: --
          產品目錄號: #22910
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 腺病毒

          載體質粒圖譜和多克隆位點信息

          pAAV-CAG-RFP載體圖譜



          載體簡介

          pAAV-CAG-RFP載體
          
          This AAV is a RFP construct driven by the ubiquitous CAG promoter.
          
          The roughly 1.8Kb CAG promoter (also known as CBA promoter or CAGGS promoter) is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian cells. The CAG promoter is composed of the following regulatory elements: (C) cytomegalovirus (CMV) early enhancer element; (A) the promoter region, the first exon, and the first intron of chicken beta-Actin gene, and (G) the splice acceptor of the rabbit beta-Globin gene. Like the EF1a promoter, the CAG promoter is commonly used as an alternative for the CMV promoter, from which the expression is decreased due to methylation/silencing. In many cell types tested, the CAG and EF1a promoters give much higher levels of expression than other commonly used cellular promoters such as the UBC and PGK promoters.
          
          RFP has an Excitation/Emission spectrum of 500nm/580nm. Other fluorescent proteins with similar spectrums include Kusabira Orange , mOrange, mOrange2, dTomato, tdTomato, DsRed, DsRed2, TurboRFP, and TagRFP
          

          載體序列

          LOCUS       pAAV-CAG-RFP Sequencing Result	7218 bp 	DNA	SYN
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     ORIGDB|GenBank
          COMMENT     VNTAUTHORNAME|biofeng.com|
          COMMENT     VNTNAME|pAAV-CAG-RFP|
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..7218
                               /organism="pAAV-CAG-RFP Sequencing Result"
                               /mol_type="other DNA"
               CDS             complement(108..716)
                               /label="ORF frame 2"
                               /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                               HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                               PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                               QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
               misc_feature    1051..1070
                               /label="pCAG_F_primer"
                               /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                               HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                               PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                               QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
               misc_feature    1291..1674
                               /label="bGlob_int"
                               /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                               HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                               PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                               QRALPEPLKAFTQPQKRNKPSLNQALITARRRRAVPPAHLWE*"
               CDS             1600..2454
                               /label="ORF frame 1"
                               /translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
                               GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
                               GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
                               DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
                               GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
                               PPVPVAAK*"
               misc_feature    1711..1727
                               /label="pBluescriptSK_primer"
                               /translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
                               GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
                               GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
                               DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
                               GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
                               PPVPVAAK*"
               CDS             1748..2443
                               /label="ORF frame 2"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               gene            1766..2443
                               /label="DsRed_Express_2"
                               /gene="DsRed_Express_2"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               gene            1769..2440
                               /label="DsRed_Express"
                               /gene="DsRed_Express"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               misc_feature    complement(1946..1966)
                               /label="dsRed1_N_primer"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               misc_feature    2358..2381
                               /label="dsRed1_C_primer"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               misc_feature    complement(2486..2503)
                               /label="Sp6_primer"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               promoter        complement(2487..2504)
                               /label="Sp6_promoter"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               misc_feature    complement(2523..2540)
                               /label="BGH_rev_primer"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               terminator      2526..2717
                               /label="bGH_PA_terminator"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               rep_origin      3301..3607
                               /label="f1_origin"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               misc_feature    4398..4420
                               /label="pGEX_3_primer"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               promoter        4579..4607
                               /label="AmpR_promoter"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               gene            4649..5509
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                               EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                               MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                               GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                               QYERAEGRHHLFL*"
               CDS             4649..5509
                               /label="ORF frame 2"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               rep_origin      5664..6283
                               /label="pBR322_origin"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               misc_feature    6666..6953
                               /label="CAG_enhancer"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC
             61 GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG
            121 TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC
            181 TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG
            241 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCT
            301 CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCA
            361 GTGTGCGCGA GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG GTGCGGGGGG GGCTGCGAGG
            421 GGAACAAAGG CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGT
            481 CGGTCGGGCT GCAACCCCCC CTGCACCCCC CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT
            541 CGGGTGCGGG GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC
            601 GGCAGGTGGG GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG
            661 GGCGCGGCGG CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC
            721 TTTTATGGTA ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGTGCGGAGC
            781 CGAAATCTGG GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG
            841 CCGGCAGGAA GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC
            901 TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA
            961 GGGCGGGGTT CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC
           1021 ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC
           1081 ATTTTGGCAA AGAATTGTGG CGGCCCGACC GCGGGCTAGC GGCGCGCCGA GCTCGGATCC
           1141 TGAGAACTTC AGGGTGAGTC TATGGGACCC TTGATGTTTT CTTTCCCCTT CTTTTCTATG
           1201 GTTAAGTTCA TGTCATAGGA AGGGGAGAAG TAACAGGGTA CACATATTGA CCAAATCAGG
           1261 GTAATTTTGC ATTTGTAATT TTAAAAAATG CTTTCTTCTT TTAATATACT TTTTTGTTTA
           1321 TCTTATTTCT AATACTTTCC CTAATCTCTT TCTTTCAGGG CAATAATGAT ACAATGTATC
           1381 ATGCCTCTTT GCACCATTCT AAAGAATAAC AGTGATAATT TCTGGGTTAA GGCAATAGCA
           1441 ATATTTCTGC ATATAAATAT TTCTGCATAT AAATTGTAAC TGATGTAAGA GGTTTCATAT
           1501 TGCTAATAGC AGCTACAATC CAGCTACCAT TCTGCTTTTA TTTTATGGTT GGGATAAGGC
           1561 TGGATTATTC TGAGTCCAAG CTAGGCCCTT TTGCTAATCA TGTTCATACC TCTTATCTTC
           1621 CTCCCACAGC TCCTGGGCAA CGTGCTGGTC TGTGTGCTGG CCCATCACTT TGGCAAAGCA
           1681 CGTGAATTCG AAGATCTGGC CGCCTCGGCC TCTAGAACTA GTGGATCCCC CGGGCTGCAG
           1741 GAATTCGATG CTACCGGTCG CCACCATGGC CTCCTCCGAG GACGTCATCA AGGAGTTCAT
           1801 GCGCTTCAAG GTGCGCATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA
           1861 GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG
           1921 CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT CCAAGGTGTA
           1981 CGTGAAGCAC CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA
           2041 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC
           2101 CCTGCAGGAC GGCTCCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT TCCCCTCCGA
           2161 CGGCCCCGTA ATGCAGAAGA AGACTATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC
           2221 CCGCGACGGC GTGCTGAAGG GCGAGATCCA CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA
           2281 CTACCTGGTG GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA
           2341 CTACTACGTG GACTCCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA
           2401 GCAGTACGAG CGCGCCGAGG GCCGCCACCA CCTGTTCCTG TAGCGGCCAA ATGAATTCCC
           2461 AAACCGTACG GGAAACGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG CTCGCTGATC
           2521 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC
           2581 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC
           2641 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG
           2701 GGAGGATTGG GAAGACAATA GCGCGGCCGC TCTAGAGCAT GGCTACGTAG ATAAGTAGCA
           2761 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT
           2821 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
           2881 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
           2941 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGATTCC GTTGCAATGG
           3001 CTGGCGGTAA TATTGTTCTG GATATTACCA GCAAGGCCGA TAGTTTGAGT TCTTCTACTC
           3061 AGGCAAGTGA TGTTATTACT AATCAAAGAA GTATTGCGAC AACGGTTAAT TTGCGTGATG
           3121 GACAGACTCT TTTACTCGGT GGCCTCACTG ATTATAAAAA CACTTCTCAG GATTCTGGCG
           3181 TACCGTTCCT GTCTAAAATC CCTTTAATCG GCCTCCTGTT TAGCTCCCGC TCTGATTCTA
           3241 ACGAGGAAAG CACGTTATAC GTGCTCGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC
           3301 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC
           3361 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
           3421 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC
           3481 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG
           3541 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT
           3601 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
           3661 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
           3721 ATATTAACGC TTACAATTTA AATATTTGCT TATACAATCT TCCTGTTTTT GGGGCTTTTC
           3781 TGATTATCAA CCGGGGTACA TATGATTGAC ATGCTAGTTT TACGATTACC GTTCATCGAT
           3841 TCTCTTGTTT GCTCCAGACT CTCAGGCAAT GACCTGATAG CCTTTGTAGA GACCTCTCAA
           3901 AAATAGCTAC CCTCTCCGGC ATGAATTTAT CAGCTAGAAC GGTTGAATAT CATATTGATG
           3961 GTGATTTGAC TGTCTCCGGC CTTTCTCACC CGTTTGAATC TTTACCTACA CATTACTCAG
           4021 GCATTGCATT TAAAATATAT GAGGGTTCTA AAAATTTTTA TCCTTGCGTT GAAATAAAGG
           4081 CTTCTCCCGC AAAAGTATTA CAGGGTCATA ATGTTTTTGG TACAACCGAT TTAGCTTTAT
           4141 GCTCTGAGGC TTTATTGCTT AATTTTGCTA ATTCTTTGCC TTGCCTGTAT GATTTATTGG
           4201 ATGTTGGAAT CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG
           4261 CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA
           4321 CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG
           4381 ACAAGCTGTG ACCGTCTccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
           4441 acgcgcgagA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT
           4501 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
           4561 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
           4621 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
           4681 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
           4741 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
           4801 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
           4861 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
           4921 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
           4981 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
           5041 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
           5101 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
           5161 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
           5221 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
           5281 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
           5341 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG
           5401 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
           5461 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
           5521 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
           5581 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
           5641 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
           5701 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
           5761 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
           5821 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
           5881 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
           5941 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
           6001 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
           6061 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
           6121 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
           6181 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
           6241 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
           6301 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
           6361 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
           6421 CAGCGAGTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA TCACTAGGGG
           6481 TTCCTTGTAG TTAATGATTA ACCCGCCATG CTACTTATCT ACGTAGCCAT GCTCTAGAGG
           6541 ATCCTTCGAA AAGCTTGTAT ACGGCCGGCC ACGCGTTCGC CACTCGACAT TGATTATTGA
           6601 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC
           6661 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT
           6721 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC
           6781 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC
           6841 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT
           6901 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA
           6961 CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC
           7021 CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG
           7081 GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA
           7141 GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC
           7201 GGCGGCGGCG GCGGCCCT
          //
          
          

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