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          pAAV-CAG-Fluc

          價格:2500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: --
          載體名稱: pAAV-CAG-Fluc 、pAAV-CAG-luc
          質粒類型: 腺病毒載體;熒光素酶報告載體;熒光蛋白報告載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: CAG (CMV enhancer/chicken β-actin promoter)
          載體大小: 8323 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: 螢火蟲熒光素酶、橙色熒光蛋白
          克隆菌株: Stbl3 或 NEB Stble
          宿主細胞(系): 常規哺乳動物細胞系
          備注: --
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 腺病毒

          載體質粒圖譜和多克隆位點信息

          pAAV-CAG-Fluc載體圖譜



          載體簡介

          This AAV is a fLuc construct driven by the ubiquitous CAG promoter.
          
          The roughly 1.8Kb CAG promoter (also known as CBA promoter or CAGGS promoter) is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian cells. The CAG promoter is composed of the following regulatory elements: (C) cytomegalovirus (CMV) early enhancer element; (A) the promoter region, the first exon, and the first intron of chicken beta-Actin gene, and (G) the splice acceptor of the rabbit beta-Globin gene. Like the EF1a promoter, the CAG promoter is commonly used as an alternative for the CMV promoter, from which the expression is decreased due to methylation/silencing. In many cell types tested, the CAG and EF1a promoters give much higher levels of expression than other commonly used cellular promoters such as the UBC and PGK promoters.
          
          Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.
          

          載體序列

          LOCUS       KC152483                8323 bp    DNA     circular SYN 03-JUN-2013
          DEFINITION  Expression vector pAAV-CAG-Fluc, complete sequence.
          ACCESSION   KC152483
          REFERENCE   1  (bases 1 to 8323)
            AUTHORS   Chen,Y., Cao,L., Luo,C., Ditzel,D.A., Peter,J. and Sprengel,R.
            TITLE     RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
            JOURNAL   Mol Ther Nucleic Acids 2, E85 (2013)
             PUBMED   23571608
            JOURNAL   Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
                      institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
                      Germany
          FEATURES             Location/Qualifiers
               source          1..8323
                               /organism="Expression vector pAAV-CAG-Fluc"
                               /mol_type="other DNA"
                               /db_xref="taxon:1337866"
               repeat_region   19..162
                               /note="AAV2 ITR"
                               /rpt_type=inverted
                               /rpt_type=terminal
               regulatory      200..1140
                               /regulatory_class="promoter"
                               /note="CMV earlyenhancer/chicken beta actin promoter: CAG"
               misc_feature    1157..2809
                               /note="firefly luciferase"
               misc_feature    2810..2863
                               /note="2A peptide from Thosea asigna virus"
               misc_feature    2876..3724
                               /note="tTSKid"
               misc_feature    2876..3508
                               /note="Tet repressor: tetR[B/E]"
               misc_feature    3509..3526
                               /note="nuclear localization signal: NLS"
               misc_feature    3533..3718
                               /note="KRAB (Kruppel-associated box) domain; from Rat zinc
                               finger protein (kid-1)"
               misc_feature    3725..3778
                               /note="2A peptide from Thosea asigna virus"
               gene            3791..4447
                               /gene="mKO"
               CDS             3791..4447
                               /gene="mKO"
                               /codon_start=1
                               /transl_table=11
                               /product="monomeric Kusabira-orange"
                               /protein_id="AGM53878.1"
                               /db_xref="GI:507593976"
                               /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLR
                               VTMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGG
                               SASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDV
                               TMYLKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS
                               "
               ncRNA           4478..5074
                               /ncRNA_class="other"
                               /note="post-transcriptional regulatory element: WPRE"
               misc_feature    5107..5317
                               /note="bovine growth hormone polyA signal: bGH pA"
               repeat_region   complement(5327..5470)
                               /note="AAV2 ITR"
                               /rpt_type=inverted
                               /rpt_type=terminal
               gene            6659..7321
                               /gene="Bla"
               CDS             6659..7321
                               /gene="Bla"
                               /note="ampicillin resistance"
                               /codon_start=1
                               /transl_table=11
                               /product="beta-lactamase"
                               /protein_id="AGM53877.1"
                               /db_xref="GI:507593975"
                               /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                               LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
                               EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
                               WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
                               HW"
          ORIGIN      
                  1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
                 61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
                121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
                181 ttctagtcct agggatgtac cattgacgtc aataatgacg tatgttccca tagtaacgcc
                241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
                301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
                361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
                421 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
                481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
                541 tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
                601 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
                661 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
                721 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
                781 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
                841 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
                901 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcggggct
                961 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
               1021 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
               1081 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc
               1141 tagtcgattc gaattcatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
               1201 tccgctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
               1261 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
               1321 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
               1381 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
               1441 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
               1501 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
               1561 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
               1621 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
               1681 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
               1741 aatgaactcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc
               1801 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
               1861 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
               1921 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg
               1981 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt
               2041 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
               2101 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg
               2161 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
               2221 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
               2281 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc
               2341 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
               2401 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt
               2461 tgaccgcttg aagtctttaa ttaaatacaa aggataccag gtggcccccg ctgaattgga
               2521 gtcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga
               2581 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
               2641 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
               2701 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
               2761 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgctcg agggcagagg
               2821 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctgcaccgg gatccatgtc
               2881 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
               2941 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
               3001 ttggcacgtg cgcaacaagc agactcttat gaacatgctt tcagaggcaa tactggcgaa
               3061 gcatcacacc cgttcagcac cgttaccgac tgagagttgg cagcagtttc tccaggaaaa
               3121 tgctctgagt ttccgtaaag cattactggt ccatcgtgat ggagcccgat tgcatatagg
               3181 gacctctcct acgccccccc agtttgaaca agcagaggcg caactacgct gtctatgcga
               3241 tgcagggttt tcggtcgagg aggctctttt cattctgcaa tctatcagcc attttacgtt
               3301 gggtgcagta ttagaggagc aagcaacaaa ccagatagaa aataatcatg tgatagacgc
               3361 tgcaccacca ttattacaag aggcatttaa tattcaggcg agaacctctg ctgaaatggc
               3421 cttccatttc gggctgaaat cattaatatt tggattttct gcacagttag atgaaaaaaa
               3481 gcatacaccc attgaggatg gtaataaacc aaaaaagaag agaaagctag cagtgtcagt
               3541 gacatttgaa gatgtggctg tgctctttac tcgggacgag tggaagaagc tggatctgtc
               3601 tcagagaagc ctgtaccgtg aggtgatgct ggagaattac agcaacctgg cctccatggc
               3661 aggattcctg tttaccaaac caaaggtgat ctccctgttg cagcaaggag aggacccgta
               3721 tctcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgc
               3781 accgggatcc atggtgagtg tgattaaacc agaaatgaag atgaggtact acatggacgg
               3841 ctccgtcaat gggcatgagt tcacaattga aggtgaaggc acaggcagac cttacgaggg
               3901 acatcaagag atgacactac gcgtcacaat ggccaagggc gggccaatgc ctttcgcgtt
               3961 tgacttagtg tcacacgtgt tctgttacgg ccacagacct tttactaaat atccagaaga
               4021 gataccagac tatttcaaac aagcatttcc tgaaggcctg tcatgggaaa ggtcgttgga
               4081 gttcgaagat ggtgggtccg cttcagtcag tgcgcatata agccttagag gaaacacctt
               4141 ctaccacaaa tccaaattta ctggggttaa ctttcctgcc gatggtccta tcatgcaaaa
               4201 ccaaagtgtt gattgggagc catcaaccga gaaaattact gccagcgacg gagttctgaa
               4261 gggtgatgtt acgatgtacc taaaacttga aggaggcggc aatcacaaat gccaattcaa
               4321 gactacttac aaggcggcaa aaaagattct taaaatgcca ggaagccatt acatcagcca
               4381 tcgcctcgtc aggaaaaccg aaggcaacat tactgagctg gtagaagatg cagtagctca
               4441 ttcctgatgt acaagtaaag cggccgcaag cttatcgata atcaacctct ggattacaaa
               4501 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
               4561 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
               4621 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
               4681 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
               4741 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
               4801 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
               4861 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
               4921 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
               4981 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
               5041 cggatctccc tttgggccgc ctccccgcct gatcgatacc gtcgactaga gctcgctgat
               5101 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
               5161 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
               5221 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
               5281 gggaggattg ggaagacaat agcaggcatg ctggggagag atctgaggaa cccctagtga
               5341 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
               5401 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
               5461 gagtggccaa cccccccccc cccccccctg cagcctggcg taatagcgaa gaggcccgca
               5521 ccgatcgccc ttcccaacag ttgcgtagcc tgaatggcga atggcgcgac gcgccctgta
               5581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
               5641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
               5701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
               5761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
               5821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
               5881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
               5941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
               6001 acaaaatatt aacgtttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
               6061 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
               6121 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
               6181 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
               6241 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
               6301 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
               6361 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
               6421 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
               6481 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
               6541 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
               6601 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
               6661 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
               6721 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
               6781 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
               6841 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
               6901 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
               6961 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
               7021 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
               7081 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
               7141 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
               7201 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
               7261 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
               7321 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
               7381 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
               7441 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
               7501 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
               7561 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
               7621 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
               7681 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
               7741 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
               7801 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
               7861 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
               7921 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
               7981 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
               8041 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
               8101 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
               8161 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
               8221 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
               8281 gcctctcccc gcgcgttggc cgattcatta atgcaggctg cag
          //
          

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