免费高清视频 成人免费视频 成年在线视频免费观看视频

    <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

      1. <pre id="q7rs8"></pre>
        <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
        <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

          pIκB-EGFP

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Stratagene
          載體名稱: pIκB-EGFP
          質粒類型: 信號通路報告系統載體
          高拷貝/低拷貝: --
          啟動子: CMV
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 5.7kb
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: EGFP
          載體抗性: 卡納霉素(Kanamycin)
          篩選標記: 新霉素(Neomycin)
          備注: --
          產品目錄號: 6919-1
          穩定性: --
          組成型: --
          病毒/非病毒: --

          載體質粒圖譜和多克隆位點信息

          pIκB-EGFP載體圖譜



          pIκB-EGFP載體特征

          載體簡介

          
          The pIκB-EGFP Vector encodes the IκB-EGFP Signaling Probe, which is a fusion of enhanced green
          fluorescent protein (EGFP) and IκB. IκB is an inhibitor of NFκB, a transcription factor involved in the
          immune response and inflammatory diseases. When the NFκB pathway is inactive, IκB and NFκB
          exist as an inactive complex in the cytosol. Upon stimulation, IκB is degraded. In cells transfected
          with pIκB-EGFP, degradation of the IκB-EGFP fusion protein is observed as a decrease in EGFP
          fluorescence (1). The IκB-EGFP Signaling Probe is constitutively expressed and resides in the
          cytosol.
          EGFP is a red-shifted, human codon-optimized variant of GFP (2–6) that has been engineered for
          brighter fluorescence and higher expression in mammalian cells. Its excitation maximum is 488 nm
          and emission maximum is 509 nm. For more information on the properties of EGFP, please refer to
          the BD Living Colors? User Manual (PT2040-1) included with the vector.
          Expression of the IκB-EGFP is driven by the human CMV immediate-early promoter. The SV40 poly-A
          sequence directs proper processing of the 3' end of the fusion construct. The vector backbone also
          contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A
          neomycin resistance cassette (Neor) allows kanamycin selection in E. coli and neomycin (G418)
          selection in eukaryotic cells. This cassette consists of the SV40 early promoter, the neomycin/
          kanamycin resistance gene of Tn5, and poly-A signals from the Herpes simplex virus thymidine
          kinase (HSV TK) gene. The vector backbone also provides a pUC origin of replication for
          propagation in E. coli and an f1 origin for single-stranded DNA production.
          

          載體序列

          載體質粒圖譜pdf版和相關資料下載

          載體應用舉例

          分享到:

          全部載體分類

          相關載體

          相關文章

          相關問答

          Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng
          歡迎您的訪問和咨詢....
            <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

              1. <pre id="q7rs8"></pre>
                <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
                <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

                  免费高清视频 成人免费视频 成年在线视频免费观看视频

                  品牌简介

                  免费高清视频 成人免费视频 成年在线视频免费观看视频