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        <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

          pGS-21a

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          pGS21a載體基本信息

          出品公司: GenScript
          載體名稱: pGS-21a
          質粒類型: 克隆載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: --
          啟動子: --
          載體大小: 6169 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: --
          克隆菌株: --
          宿主細胞(系): --
          備注: --
          產品目錄號: --
          穩定性: --
          組成型/誘導型: --
          病毒/非病毒: --

          pGS21a載體質粒圖譜和多克隆位點信息

          pGS-21a載體圖譜

          pGS21a載體簡介

          pGS21a載體序列

          LOCUS       pGS-21a                6169 bp ds-DNA     circular SYN 25-NOV-2013
          DEFINITION  Bacterial vector for expression of 6xHis- and GST-tagged proteins. 
                      Two 6xHis sequences are encoded in the vector.
          ACCESSION   .
          VERSION     .
          KEYWORDS    pGS-21a
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 6169)
            AUTHORS   GenScript
            TITLE     Direct Submission
            JOURNAL   Exported Thursday, February 6, 2020 from SnapGene Viewer 3.1.4
                      http://www.snapgene.com
          FEATURES             Location/Qualifiers
               source          1..6169
                               /organism="synthetic DNA construct"
                               /lab_host="Escherichia coli"
                               /mol_type="other DNA"
               promoter        1..19
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               protein_bind    20..44
                               /bound_moiety="lac repressor encoded by lacI"
                               /note="lac operator"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               CDS             89..91
                               /codon_start=1
                               /product="start codon"
                               /note="ATG"
                               /translation="M"
               CDS             101..118
                               /codon_start=1
                               /product="6xHis affinity tag"
                               /note="6xHis"
                               /translation="HHHHHH"
               CDS             128..781
                               /codon_start=1
                               /product="glutathione S-transferase from Schistosoma 
                               japonicum"
                               /note="GST"
                               /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                               FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                               GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                               YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
               CDS             818..832
                               /codon_start=1
                               /product="enterokinase recognition and cleavage site"
                               /note="enterokinase site"
                               /translation="DDDDK"
               misc_feature    834..892
                               /note="MCS"
                               /note="multiple cloning site"
               CDS             893..910
                               /codon_start=1
                               /product="6xHis affinity tag"
                               /note="6xHis"
                               /translation="HHHHHH"
               terminator      977..1024
                               /note="T7 terminator"
                               /note="transcription terminator for bacteriophage T7 RNA 
                               polymerase"
               rep_origin      1061..1516
                               /direction=RIGHT
                               /note="f1 ori"
                               /note="f1 bacteriophage origin of replication; arrow 
                               indicates direction of (+) strand synthesis"
               promoter        1543..1647
                               /gene="bla"
                               /note="AmpR promoter"
               CDS             1648..2508
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
               rep_origin      2679..3267
                               /direction=RIGHT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(3697..3888)
                               /codon_start=1
                               /gene="rop"
                               /product="Rop protein, which maintains plasmids at low copy
                               number"
                               /note="rop"
                               /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                               DELYRSCLARFGDDGENL"
               CDS             complement(4697..5779)
                               /codon_start=1
                               /gene="lacI"
                               /product="lac repressor"
                               /note="lacI"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                               /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                               NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                               EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                               EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                               MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                               YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                               ALADSLMQLARQVSRLESGQ"
               promoter        complement(5780..5857)
                               /gene="lacI"
                               /note="lacI promoter"
          ORIGIN
                  1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
                 61 tgtttaactt taagaaggag atatacatat gtctggttct catcatcatc atcatcatag
                121 cagcggtatg tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg
                181 acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg
                241 tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta
                301 tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa
                361 gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc
                421 ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct
                481 caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg
                541 tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc
                601 tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg
                661 ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta
                721 tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa
                781 atcggatctg ggccacacag gccatagatc tggtaccgac gacgacgaca aggccatggc
                841 tgatatcgga tccgaattcg agctcgtcga caagcttgcg gccgcactcg agcaccacca
                901 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
                961 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
               1021 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
               1081 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
               1141 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
               1201 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
               1261 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
               1321 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
               1381 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
               1441 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
               1501 ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
               1561 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
               1621 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
               1681 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
               1741 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
               1801 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
               1861 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
               1921 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
               1981 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
               2041 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
               2101 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
               2161 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
               2221 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
               2281 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
               2341 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
               2401 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
               2461 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
               2521 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
               2581 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
               2641 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
               2701 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
               2761 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
               2821 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
               2881 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
               2941 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
               3001 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
               3061 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
               3121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
               3181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
               3241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
               3301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
               3361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
               3421 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
               3481 ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg
               3541 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
               3601 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
               3661 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
               3721 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
               3781 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
               3841 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
               3901 gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
               3961 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
               4021 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
               4081 taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
               4141 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
               4201 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
               4261 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
               4321 cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc
               4381 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
               4441 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
               4501 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
               4561 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
               4621 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
               4681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
               4741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt
               4801 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga
               4861 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg
               4921 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt
               4981 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat
               5041 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt
               5101 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc
               5161 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc
               5221 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg
               5281 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa
               5341 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg
               5401 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac
               5461 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg
               5521 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg
               5581 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt
               5641 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg
               5701 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt
               5761 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg
               5821 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
               5881 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
               5941 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
               6001 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
               6061 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
               6121 acgatgcgtc cggcgtagag gatcgagatc gatctcgatc ccgcgaaat
          //
          
          
          

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