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          SuperTopFlash

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: 愛迪基因
          載體名稱: SuperTopFlash
          質粒類型: 信號通路報告系統載體
          高拷貝/低拷貝: 高拷貝
          啟動子: --
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 4997bp
          5' 測序引物及序列: RVPrimer3: CTAGCAAAATAGGCTGTCCC
          3' 測序引物及序列:
          LucNRev: CCTTATGCAGTTGCTCTCC
          載體標簽: --
          載體抗性: Ampicillin
          篩選標記: --
          備注: 克隆菌株DH5alpha
          產品目錄號: 12456
          穩定性: --
          組成型: --
          病毒/非病毒: --

          載體質粒圖譜和多克隆位點信息

          SuperTopFlash載體圖譜



          載體簡介

          載體序列

          LOCUS       M50 Super 8x TOPFlash	4997 bp 	DNA	SYN
          DEFINITION  M50 Super 8x TOPFlash
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..4997
                               /organism="M50 Super 8x TOPFlash"
                               /mol_type="other DNA"
               gene            267..1919
                               /label="firefly_Luciferase"
                               /gene="firefly_Luciferase"
               CDS             267..1919
                               /label="ORF frame 3"
                               /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                               AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                               APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                               FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                               FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                               LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                               GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                               RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                               PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                               AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
               misc_feature    complement(333..351)
                               /label="LucNrev_primer"
                               /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                               AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                               APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                               FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                               FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                               LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                               GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                               RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                               PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                               AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
               misc_feature    complement(1971..1990)
                               /label="EBV_rev_primer"
                               /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                               AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                               APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                               FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                               FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                               LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                               GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                               RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                               PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                               AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
               gene            complement(3259..4119)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                               AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                               APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                               FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                               FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                               LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                               GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                               RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                               PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                               AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
               CDS             complement(3259..4119)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(4161..4189)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               rep_origin      4268..4574
                               /label="f1_origin"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               CDS             complement(4784..404)
                               /label="ORF frame 1"
                               /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                               FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                               DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                               CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
               misc_feature    4837..4990
                               /label="TB"
                               /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                               FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                               DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                               CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
               terminator      4837..4885
                               /label="synth_PA_terminator"
                               /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                               FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                               DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                               CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
               terminator      4837..4990
                               /label="up_PA_terminator"
                               /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                               FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                               DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                               CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
               misc_feature    4939..4958
                               /label="RVprimer3_primer"
                               /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                               FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                               DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                               CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
          ORIGIN
              1 ggtaccgagc tcttacgcga gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg
             61 ggtaagatca aaggcgcgag atcaaagggg gtaagatcaa agggggtaag atcaaagggg
            121 gtaagatcaa aggggcgcgc ccgcgtgcta gcccgggctc gagatctaga ctctagaggg
            181 tatataatgg aagctcgaat tccagcttgg cattccggta ctgttggtaa aaagcttggc
            241 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
            301 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
            361 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
            421 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
            481 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
            541 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
            601 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
            661 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
            721 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
            781 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
            841 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
            901 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
            961 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
           1021 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
           1081 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
           1141 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
           1201 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
           1261 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
           1321 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
           1381 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
           1441 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
           1501 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
           1561 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
           1621 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
           1681 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
           1741 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
           1801 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
           1861 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
           1921 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
           1981 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
           2041 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
           2101 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
           2161 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
           2221 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
           2281 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
           2341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
           2401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
           2461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
           2521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
           2581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
           2641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
           2701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
           2761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
           2821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
           2881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
           2941 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
           3001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
           3061 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
           3121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
           3181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
           3241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
           3301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
           3361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
           3421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
           3481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
           3541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
           3601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
           3661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
           3721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
           3781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
           3841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
           3901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
           3961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
           4021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
           4081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
           4141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
           4201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
           4261 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
           4321 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
           4381 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
           4441 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
           4501 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
           4561 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
           4621 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
           4681 taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca actgttggga
           4741 agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag
           4801 taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta
           4861 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
           4921 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
           4981 acatttctct atcgata
          //
          
          

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