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          pQCXIN

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Clontech
          載體名稱: pQCXIN
          質粒類型: 逆轉錄病毒載體; 雙順反子載體
          高拷貝/低拷貝: 低拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: CMV
          載體大小: 7381 bp
          5' 測序引物及序列: 5'-ACGCCATCCACGCTGTTTTGACCT-3'
          3' 測序引物及序列: 5'-AAGCGGCTTCGGCCAGTAACGTTA-3'
          載體標簽: 無標簽
          載體抗性: 氨芐青霉素
          篩選標記: Neomycin
          克隆菌株: DH5α, HB101
          宿主細胞(系): 常規細胞系
          備注: pQCXIN載體含有IRES元件,是雙順反子載體,目的基因與Neomycin抗性基因共轉錄。
          產品目錄號: 631514
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 逆轉錄病毒

          載體質粒圖譜和多克隆位點信息

          pQCXIN載體圖譜



          pQCXIN 多克隆位點

          pQCXIN 載體特征

          載體簡介

          pQCXIN載體簡介
          pQCXIN Retroviral Vector is a bicistronic expression vector designed to express a target gene along with the neomycin selection marker (1). Upon transfection into a packaging cell line, this vector can transiently express, or integrate and stably express a viral genomic transcript containing the CMV immediate early promoter, gene of interest, IRES and the neomycin resistance gene (Neor). The gene of interest and the neomycin resistance gene are co-translated, via the internal ribosome entry site (IRES), from a bicistronic message in mammalian cells (2, 3).
          This vector incorporates unique features including: optimization to remove promoter interference and self-inactivation. The hybrid 5' LTR consists of the cytomegalovirus (CMV) type I enhancer and the mouse sarcoma virus (MSV) promoter. This construct drives high levels of transcription in HEK 293-based packaging cell lines due, in part, to the presence of adenoviral E1A (4, 5, 6, 7) in these cells. The self-inactivating feature of the vector is provided by a deletion in the 3' LTR enhancer region (U3). During reverse transcription of the retroviral RNA, the inactivated 3' LTR is copied and replaces the 5' LTR, resulting in inactivation of the 5' LTR CMV enhancer sequences. This may reduce the phenomenon known as promoter interference (8) and allow more efficient expression.
          Also included in the viral genomic transcript are the necessary viral RNA processing elements including the LTRs, packaging signal (Psi+), and tRNA primer binding site. pQCXIN also contains a bacterial origin of replication and E. coli Ampr gene for propagation and selection in bacteria.
          
          pQCXIN載體的使用
          pQCXIN is designed to deliver and express a gene along with the neomycin resistance marker from a bicistronic message. The design is optimized to produce high titers via the PCMV IE in the packaging cell line. The bicistronic transcript makes it unnecessary to screen the transformants since the neomycin resistance is expressed in concert with the DNA inserted into the multiple cloning site.
          Once transfected into the packaging cell line (such as the RetroPack™ PT67 Cell Line (Cat. No.631510) AmphoPack293, EcoPack2-293, or Pantropic System), RNA from the vector is packaged into infectious, replication-incompetent retroviral particles since pQCXIN lacks structural genes (gag, pol, and env) necessary for particle formation and replication; however, these genes are stably integrated as part of the packaging cell genome. Once a high titer clone is selected, these retroviral particles can infect target cells and transmit the gene of interest but cannot replicate within these cells due to the absence of viral structural genes. The separate introductioin and integration of the structural genes into the packaging cell line minimizes the chances of producing replication-competent virus due to recomination events during cell proliferation.
          

          載體序列

          LOCUS       pQCXIN	7381 bp 	DNA	SYN
          DEFINITION  pQCXIN
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..7381
                               /organism="pQCXIN"
                               /mol_type="other DNA"
               promoter        2..506
                               /label="CMV_immearly_promoter"
               misc_feature    6..688
                               /label="5_LTR"
               misc_feature    9..296
                               /label="CAG_enhancer"
               misc_feature    463..483
                               /label="CMV_fwd_primer"
               misc_feature    758..1567
                               /label="psi_plus_pack"
               misc_feature    1154..1561
                               /label="gag"
               misc_feature    1497..1519
                               /label="pLXSN_5_primer"
               misc_feature    1497..1519
                               /label="MSCV_primer"
               misc_feature    1535..1551
                               /label="pBABE_5_primer"
               misc_feature    1608..1895
                               /label="CAG_enhancer"
               promoter        1613..2105
                               /label="CMV_immearly_promoter"
               misc_feature    2062..2082
                               /label="CMV_fwd_primer"
               promoter        2063..2132
                               /label="CMV_promoter"
               promoter        2075..2194
                               /label="CMV2_promoter"
               misc_feature    2106..2125
                               /label="pCEP_fwd_primer"
               misc_feature    2108..2132
                               /label="LNCX_primer"
               misc_feature    2289..2851
                               /label="IRES"
               CDS             2876..3670
                               /label="ORF frame 2"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               gene            2891..3667
                               /label="NeoR/KanR"
                               /gene="NeoR/KanR"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               misc_feature    complement(4615..4637)
                               /label="pGEX_3_primer"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               misc_feature    complement(4773..4793)
                               /label="pBABE_3_primer"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               misc_feature    complement(4779..4994)
                               /label="SV40_enhancer"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               promoter        4791..5059
                               /label="SV40_promoter"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               rep_origin      4958..5035
                               /label="SV40_origin"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               misc_feature    5020..5039
                               /label="SV40pro_F_primer"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               gene            complement(6159..7019)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               CDS             complement(6159..7019)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(7061..7089)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        7311..506
                               /label="CMV_immearly_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               misc_feature    7375..688
                               /label="5_LTR"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
             61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
            121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
            181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
            241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
            301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
            361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
            421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
            481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
            541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
            601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
            661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
            721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
            781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
            841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
            901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
            961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
           1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
           1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
           1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
           1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
           1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
           1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
           1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
           1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
           1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
           1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
           1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
           1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
           1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
           1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
           1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
           1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
           1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
           2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
           2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
           2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
           2221 cgctgcagga attgatccgc ggccgcaccg gtaggcctcg tacgcttaat taacggatcc
           2281 ggaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
           2341 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
           2401 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
           2461 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
           2521 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
           2581 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
           2641 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
           2701 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
           2761 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
           2821 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caaccatggc
           2881 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
           2941 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
           3001 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
           3061 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
           3121 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
           3181 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
           3241 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
           3301 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
           3361 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
           3421 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
           3481 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
           3541 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
           3601 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
           3661 gttcttctga gcgggatcgg ctagagtcga cccggacggg acgctcctgc gcctgataca
           3721 gaacgaattg cttgcaggca tctcatgagt gtgtcttccc gttttccgcc tgaggtcact
           3781 gcgtggatgg agcgctggcg cctgctgcgc gacggcgagc tgctcaccac ccactcgagg
           3841 gctgcagcgc tgcagaggcc gagtgcagaa ctgctccaaa gggacctcaa ggctttccga
           3901 gggacactag gctgactcca tcgagccagt gtagagataa gcttatcgat tagtccaatt
           3961 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
           4021 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
           4081 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
           4141 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
           4201 tggaacaggg tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
           4261 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
           4321 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
           4381 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
           4441 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
           4501 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
           4561 caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
           4621 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
           4681 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
           4741 acgtagcgat agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa
           4801 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
           4861 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
           4921 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
           4981 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
           5041 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
           5101 ttgcaaaaag cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac
           5161 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
           5221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
           5281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
           5341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
           5401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
           5461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
           5521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
           5581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
           5641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
           5701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
           5761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
           5821 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
           5881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
           5941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
           6001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
           6061 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
           6121 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
           6181 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
           6241 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
           6301 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
           6361 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
           6421 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
           6481 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
           6541 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
           6601 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
           6661 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
           6721 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
           6781 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
           6841 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
           6901 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
           6961 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
           7021 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
           7081 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
           7141 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
           7201 atcacgaggc cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat
           7261 cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta
           7321 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
           7381 t
          //
          

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