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          pMSCVneo

          價格:1200元

          聯系方式:I47-825O-882O

          買家導航

          pMSCV-Neo載體基本信息

          出品公司: Clontech
          載體名稱: pMSCVneo, pMSCV-neo, pMSCV neo
          質粒類型: 哺乳動物細胞逆病毒基因表達載體
          高拷貝/低拷貝: 低拷貝
          啟動子: LTR
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 6436bp
          5' 測序引物及序列: MSCV-F: CCCTTGAACCTCCTCGTTCGACC
          3' 測序引物及序列: pMSCV-R: GAGACGTGCTACTTCCATTTGTC
          載體標簽: --
          載體抗性: 氨芐
          篩選標記: 新霉素(Neomycin)
          備注: 逆病毒表達載體,優化后,有利于在造血干細胞中和癌細胞中進行基因表達。
          產品目錄號: 634401
          穩定性: 穩表達
          組成型: 組成型
          病毒/非病毒: 逆病毒

          pMSCV-Neo載體質粒圖譜和多克隆位點信息

          pMSCVneo載體圖譜



          pMSCVneo多克隆位點

          RNAi-Ready pSIREN-RetroQ載體特征

          pMSCV-Neo載體簡介

          pMSCV-Neo載體序列

          LOCUS       pMSCVneo   6436 bp    DNA   circular             4-DEC-2009
          COMMENT     Created by Clontech Laboratories Inc. 
          COMMENT     Cat. No. 634401,631461
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               misc_signal     516..1405
                               /label=Extended\packaging\signal
               promoter        1429..1937
                               /label=Phosphoglycerate\Kinase\Promoter
               CDS             1951..2751
                               /label=Neo(R)
               LTR             2859..3342
                               /label=3'\PCMV\LTR
               LTR             1..515
                               /label=5'\MMSV\LTR
               CDS             complement(4645..5502)
                               /label=Amp(R)
               rep_origin      3882..3882
                               /label=pUC\origin
               splicing_signal 519..522
                               /label=Splice\Donor
               splicing_signal 1397..1399
                               /label=Splice\Acceptor
          BASE COUNT     1458 a      1758 c      1708 g      1512 t 
          ORIGIN
                  1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
                 61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
                121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
                181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
                241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
                301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
                361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
                421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
                481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
                541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
                601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
                661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
                721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
                781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
                841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
                901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
                961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
               1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
               1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
               1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
               1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
               1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
               1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
               1381 ccctcactcc ttctctaggc gccggaattc gttaacctcg agagatctaa ttctaccggg 
               1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca 
               1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc 
               1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga 
               1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc 
               1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg 
               1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg 
               1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg 
               1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct 
               1921 catctccggg cctttcgacc tgcagccaat atgggatcgg ccattgaaca agatggattg 
               1981 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 
               2041 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 
               2101 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 
               2161 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 
               2221 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 
               2281 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 
               2341 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 
               2401 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 
               2461 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 
               2521 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 
               2581 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 
               2641 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 
               2701 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat 
               2761 ccgtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa 
               2821 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt 
               2881 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga 
               2941 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 
               3001 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat 
               3061 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc 
               3121 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc 
               3181 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg 
               3241 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg 
               3301 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga 
               3361 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag 
               3421 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag 
               3481 aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc 
               3541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
               3601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
               3661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
               3721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
               3781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
               3841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
               3901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
               3961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
               4021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
               4081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
               4141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
               4201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
               4261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
               4321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
               4381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
               4441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
               4501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
               4561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
               4621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
               4681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
               4741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
               4801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
               4861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
               4921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
               4981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
               5041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
               5101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
               5161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
               5221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
               5281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
               5341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
               5401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
               5461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
               5521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
               5581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 
               5641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 
               5701 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 
               5761 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 
               5821 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 
               5881 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 
               5941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
               6001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
               6061 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg 
               6121 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc 
               6181 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca 
               6241 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta 
               6301 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat 
               6361 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc 
               6421 cagaaaaagg ggggaa 
          //
          

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