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          pFB-Luc

          價格:3000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Agilent
          載體名稱: pFB-Luc
          質粒類型: 逆病毒載體
          高拷貝/低拷貝: 低拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: --
          載體大小: 6768 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記:
          克隆菌株: DH5α 等
          宿主細胞(系): 常規細胞系(293、CV-1、CHO等)
          備注: --
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 逆轉錄病毒

          載體質粒圖譜和多克隆位點信息

          pFB-Luc載體圖譜

          載體簡介

          pFB-Luc載體限制性酶切位點
          
          pFB-Luc, 6768 bp			     version 013001
          
          
          Enzymes with 1-10 cleavage sites:
          
                      #sites   --  Bp position of recognition site --
          
            AatII          2      978,   6694
            Acc65I         3      647,   1846,   4294
            AccI           3     1967,   2057,   4654
            AccIII         2     2759,   3275
            AflII          3      202,   1265,   3849
            AflIII         3      164,   2557,   4883
            AgeI           1     1840
            AhdI           6      687,    733,   1274,   4334,   4380
                                 5771
            Alw44I         4     1054,   4699,   5197,   6443
            AlwNI          5      323,    398,   3970,   4045,   5294
            ApaI           1     1238
            ApoI           5       87,   1128,   2467,   2651,   2905
            AvaI          10      577,    610,    643,   1241,   2018
                                 3120,   3725,   4224,   4257,   4290
            BamHI          1     3720
            BanII          8      580,    593,    764,   1238,   3084
                                 4227,   4240,   4411
            BbeI           4      615,   1656,   2096,   4262
            BbsI           4     2067,   3345,   3475,   6761
            BbvCI          2      453,   4100
            BciVI          8      656,   1986,   2762,   3067,   3191
                                 4303,   5092,   6619
            BglI           1     5890
            BglII          1     1678
            BpmI           5     1799,   2128,   3322,   3797,   5861
            Bpu10I         7      337,    412,    453,   1548,   3984
                                 4059,   4100
            BsaAI          4     1816,   2000,   2216,   4635
            BsaHI          9      615,    978,   1656,   2070,   2096
                                 3489,   4262,   6312,   6694
            BsaI           9      694,    715,    782,   1414,   1802
                                 4341,   4362,   4429,   5843
            BsaWI          9      138,   1840,   2759,   3258,   3275
                                 3642,   5089,   5236,   6067
            BseRI          6      718,   1068,   1561,   1600,   3604
                                 4365
            BsiEI          9      961,   1034,   1838,   3145,   3731
                                 4796,   5220,   6143,   6292
            BsiHKAI        8      580,   1054,   3525,   4227,   4699
                                 5197,   6358,   6443
            BsiWI          1     2218
            BsmBI          6      976,   1093,   1337,   1396,   1582
                                 4532
            Bsp120I        1     1238
            BspHI          3     5603,   6611,   6716
            BspLU11I       2      164,   4883
            BspMI          1     3471
            BsrBI          5     1299,   3728,   3753,   4814,   6615
            BsrDI          2     5830,   6012
            BsrGI          2     1541,   2554
            BssHII         2      563,   4210
            BssSI          3     5056,   6440,   6747
            Bst1107I       1     4654
            BstAPI         5      322,    397,   3969,   4044,   4701
            BstBI          2     2232,   3020
            BstEII         2     1346,   2671
            Bsu36I         2     1276,   2676
            Cfr10I         4     1840,   2333,   3492,   5856
            ClaI           1     3428
            DraI           3     5640,   5659,   6351
            DraIII         1     1873
            DrdI           2     4572,   4985
            DsaI           3      151,    931,   2062
            EaeI           7      827,    961,   1368,   1389,   1668
                                 3731,   6164
            EarI           7     1334,   1564,   2153,   2317,   2871
                                 4761,   6565
            Ecl136II       2      580,   4227
            Eco52I         2      961,   3731
            Eco57I         3     2892,   5410,   6458
            Eco72I         1     2000
            EcoNI          2     1647,   3677
            EcoO109I       6      499,   1477,   1924,   3242,   4146
                                 6751
            EcoRI          1     2651
            EcoRV          5      308,    383,   3400,   3955,   4030
            EheI           4      615,   1656,   2096,   4262
            FspI           1     5996
            HaeII          6      615,   1656,   2096,   4262,   4757
                                 5127
            HincII         3     2057,   3366,   6315
            KasI           4      615,   1656,   2096,   4262
            KpnI           3      647,   1846,   4294
            MscI           3      827,   1368,   1668
            MslI           4      159,   6024,   6183,   6542
            MunI           4       11,     21,   2179,   2635
            NarI           4      615,   1656,   2096,   4262
            NcoI           1     2062
            NdeI           3     1664,   1672,   4705
            NheI           5        6,     16,     26,    197,   3844
            NotI           1     3730
            NspI           4      164,   2723,   4518,   4883
            PacI           1     3384
            PpuMI          5      499,   1477,   1924,   3242,   4146
            PshAI          1     1015
            Psp1406I       2     6001,   6374
            PstI           3     1178,   1360,   6017
            PvuI           2     1034,   6143
            PvuII          4      286,    361,   3933,   4008
            SacI           2      580,   4227
            SacII          1      151
            SalI           1     2057
            SapI           2     2871,   4760
            ScaI           1     6254
            SexAI          1     1474
            SfcI           7      182,   1178,   1360,   3829,   5148
                                 5339,   6017
            SgrAI          1     3491
            SmaI           4      643,   1241,   2018,   4290
            SmlI           8      202,   1265,   3725,   3849,   4989
                                 5251,   5528,   6396
            SpeI           1      897
            SphI           1     2723
            SrfI           1     1240
            SspI           1     6578
            StyI           6      496,    706,   1504,   2062,   4143
                                 4353
            TatI           4     1541,   2554,   4689,   6254
            TfiI           5     2293,   2520,   2716,   3426,   4858
            Tsp45I         8     1282,   1491,   2565,   2626,   4538
                                 4633,   6033,   6244
            Tth111I        4      633,   1473,   4280,   4627
            VspI           1     5947
            XbaI           4      464,   2036,   2111,   4111
            XcmI           1     2792
            XhoI           1     3725
            XmaI           4      643,   1241,   2018,   4290
            XmnI           1     6371
          
          
          
          
          
          Enzymes that do NOT cut molecule:
          
          AscI        BclI        BlnI        Bpu1102I    BsaBI       
          BsgI        BsmI        BstXI       Eco47III    FseI        
          HindIII     HpaI        MluI        NaeI        NgoMIV      
          NruI        NsiI        PmeI        Ppu10I      RsrII       
          SanDI       SbfI        SfiI        SgfI        SnaBI       
          StuI        SwaI        Van91I      
          
          
          
          

          載體序列

          pFB-Luc, 6768 bp                              version 013001
          
          NOTE: The following sequence has been verified for accuracy
          at the junctions. The remainder of the sequence has been 
          obtained from existing data. 
          
             1  GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG  
            51  AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT  
           101  CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG  
           151  CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA  
           201  GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT  
           251  AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC  
           301  CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA  
           351  ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG  
           401  TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA  
           451  GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG  
           501  GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC  
           551  TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC  
           601  AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA  
           651  CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG  
           701  CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG  
           751  GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC  
           801  CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG  
           851  TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA  
           901  GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT  
           951  TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC  
          1001  CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT  
          1051  TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG  
          1101  AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA  
          1151  CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC  
          1201  TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG  
          1251  ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA  
          1301  GCGGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA  
          1351  CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC  
          1401  GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC  
          1451  ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG  
          1501  AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT  
          1551  AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC  
          1601  TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT  
          1651  CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG  
          1701  CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC  
          1751  CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT  
          1801  GGAGACCTCT GGCGGCACGT ACCAAGAACA ACTGGACCGA CCGGTGGTAC  
          1851  CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT  
          1901  AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC  
          1951  CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC  
          2001  ACGTGAAGGC TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT  
          2051  CGAATTGTCG ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC  
          2101  CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT  
          2151  ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA  
          2201  TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT  
          2251  TGGCAGAAGC TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC  
          2301  GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT TGGGCGCGTT  
          2351  ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG  
          2401  AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT GTTTGTTTCC  
          2451  AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACCAATAAT  
          2501  CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT  
          2551  CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC  
          2601  GATTTTGTAC CAGAGTCCTT TGATCGTGAC AAAACAATTG CACTGATAAT  
          2651  GAATTCCTCT GGATCTACTG GGTTACCTAA GGGTGTGGCC CTTCCGCATA  
          2701  GAACTGCCTG CGTCAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT  
          2751  CAAATCATTC CGGATACTGC GATTTTAAGT GTTGTTCCAT TCCATCACGG  
          2801  TTTTGGAATG TTTACTACAC TCGGATATTT GATATGTGGA TTTCGAGTCG  
          2851  TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTTTACGATC CCTTCAGGAT  
          2901  TACAAAATTC AAAGTGCGTT GCTAGTACCA ACCCTATTTT CATTCTTCGC  
          2951  CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG  
          3001  CTTCTGGGGG CGCACCTCTT TCGAAAGAAG TCGGGGAAGC GGTTGCAAAA  
          3051  CGCTTCCATC TTCCAGGGAT ACGACAAGGA TATGGGCTCA CTGAGACTAC  
          3101  ATCAGCTATT CTGATTACAC CCGAGGGGGA TGATAAACCG GGCGCGGTCG  
          3151  GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT GGATACCGGG  
          3201  AAAACGCTGG GCGTTAATCA GAGAGGCGAA TTATGTGTCA GAGGACCTAT  
          3251  GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG  
          3301  ACAAGGATGG ATGGCTACAT TCTGGAGACA TAGCTTACTG GGACGAAGAC  
          3351  GAACACTTCT TCATAGTTGA CCGCTTGAAG TCTTTAATTA AATACAAAGG  
          3401  ATATCAGGTG GCCCCCGCTG AATTGGAATC GATATTGTTA CAACACCCCA  
          3451  ACATCTTCGA CGCGGGCGTG GCAGGTCTTC CCGACGATGA CGCCGGTGAA  
          3501  CTTCCCGCCG CCGTTGTTGT TTTGGAGCAC GGAAAGACGA TGACGGAAAA  
          3551  AGAGATCGTG GATTACGTCG CCAGTCAAGT AACAACCGCG AAAAAGTTGC  
          3601  GCGGAGGAGT TGTGTTTGTG GACGAAGTAC CGAAAGGTCT TACCGGAAAA  
          3651  CTCGACGCAA GAAAAATCAG AGAGATCCTC ATAAAGGCCA AGAAGGGCGG  
          3701  AAAGTCCAAA TTGTAATGAG GATCCTCGAG CGGCCGCGAT CCGAGTTCTT  
          3751  CTGAGCGGGA CTCTGGGGTT CGATAAAATA AAAGATTTTA TTTAGTCTCC  
          3801  AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT  
          3851  TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA  
          3901  GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA  
          3951  ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA  
          4001  GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC  
          4051  CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC  
          4101  CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC  
          4151  CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG  
          4201  CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC  
          4251  CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC  
          4301  GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG  
          4351  TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC  
          4401  TTTCATTTGG GGGCTCGTCC GGGATCGGGA GACCCCTGCC CAGGGACCAC  
          4451  CGACCCACCA CCGGGAGGTA AGCTGGCTGC CTCGCGCGTT TCGGTGATGA  
          4501  CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC  
          4551  TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT  
          4601  GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG  
          4651  AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT  
          4701  GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC  
          4751  GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC  
          4801  GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT  
          4851  ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC  
          4901  AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT  
          4951  AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG  
          5001  GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA  
          5051  GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG  
          5101  TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG  
          5151  TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC  
          5201  ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT  
          5251  CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC  
          5301  TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT  
          5351  TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC  
          5401  TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG  
          5451  ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC  
          5501  AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT  
          5551  TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT  
          5601  GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA  
          5651  AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC  
          5701  AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT  
          5751  TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC  
          5801  GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA  
          5851  CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC  
          5901  CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA  
          5951  ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC  
          6001  AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG  
          6051  TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT  
          6101  CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT  
          6151  GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT  
          6201  GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG  
          6251  GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT  
          6301  TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC  
          6351  TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA  
          6401  GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC  
          6451  AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA  
          6501  AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT  
          6551  GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG  
          6601  GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA  
          6651  ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT  
          6701  AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG  
          6751  AGGCCCTTTC GTCTTCAA
          

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