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          RNAi-Ready pSIREN-RetroQ

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Clontech
          載體名稱: RNAi-Ready pSIREN-RetroQ
          質粒類型: 哺乳動物細胞自失活基因沉默逆病毒載體,shRNA
          高拷貝/低拷貝: 低拷貝
          啟動子: Human U6
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 6455 bp
          5' 測序引物及序列: U6-F: ATGGACTATCATATGCTTACCGTA (Clontech )
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐
          篩選標記: 嘌呤霉素 (Puromycin)
          備注:  
          產品目錄號: 631526
          穩定性: /
          組成型: 誘導型
          病毒/非病毒: 逆病毒

          載體質粒圖譜和多克隆位點信息

          RNAi-Ready pSIREN-RetroQ載體圖譜



          RNAi-Ready pSIREN-RetroQ多克隆位點

          RNAi-Ready pSIREN-RetroQ載體特征

          載體簡介

          載體序列

          LOCUS       pSIREN-RetroQ   6455 bp    DNA   circular            13-NOV-2009
          COMMENT     Created by Clontech Laboratories Inc. 
          COMMENT     Cat. No. 631526
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               misc_feature    1..727
                               /label=5'-LTR\(CMV/MSV)
               misc_feature    757..1566
                               /label=Extended\packaging\signal
               misc_feature    3161..3586
                               /label=3'\MMLV\LTR\(deletion\in\U3)
               CDS             complement(5233..6093)
                               /label=Amp(R)
               misc_feature    3866..4152
                               /label=SV40\Promoter
               promoter        1582..1839
                               /label=Human\U6\promoter
               rep_origin      complement(4472..5071)
                               /label=pUC\origin
               promoter        1852..2360
                               /label=PGK\Promoter
               CDS             2381..2980
                               /label=Puro(R)
               rep_origin      4087..4152
                               /label=SV40\origin
               misc_feature    1834..1856
                               /label=shRNA\Cloning\Site
          BASE COUNT     1458 a      1810 c      1668 g      1519 t 
          ORIGIN
                  1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
                 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
                121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
                181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
                241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
                301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
                361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
                421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
                481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
                541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
                601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
                661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
                721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
                781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
                841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
                901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
                961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
               1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
               1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
               1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
               1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
               1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
               1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
               1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
               1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
               1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
               1561 aggcgccgga attgaagatc tgggcaggaa gagggcctat ttcccatgat tccttcatat 
               1621 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca 
               1681 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt 
               1741 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat 
               1801 ttcttggctt tatatatctt gtggaaagga cgaggatccg gacaagcttc gaattctacc 
               1861 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg 
               1921 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc 
               1981 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca 
               2041 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac 
               2101 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt 
               2161 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg 
               2221 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct 
               2281 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt 
               2341 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac 
               2401 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 
               2461 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 
               2521 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
               2581 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
               2641 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
               2701 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
               2761 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
               2821 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
               2881 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 
               2941 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca 
               3001 gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgagata tcagtggtcc 
               3061 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga 
               3121 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta 
               3181 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga 
               3241 gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc ctagagaacc 
               3301 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa 
               3361 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga 
               3421 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg 
               3481 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag 
               3541 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg 
               3601 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc 
               3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
               3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
               3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc 
               3841 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 
               3901 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 
               3961 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
               4021 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
               4081 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 
               4141 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta 
               4201 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 
               4261 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 
               4321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
               4381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
               4441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
               4501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
               4561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
               4621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
               4681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
               4741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
               4801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
               4861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
               4921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
               4981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
               5041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
               5101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
               5161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
               5221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
               5281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
               5341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
               5401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
               5461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
               5521 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 
               5581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
               5641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
               5701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
               5761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
               5821 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 
               5881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
               5941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
               6001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
               6061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
               6121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
               6181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 
               6241 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga 
               6301 attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc 
               6361 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat 
               6421 tacggggtca ttagttcata gcccatatat ggagt 
          //
          

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