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          pSico PGK Puro

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司:  
          載體名稱: pSico PGK puro, pSico-PGK-puro
          質粒類型: 哺乳動物細胞慢病毒表達載體,RNAi, Cre/loxP
          高拷貝/低拷貝: 高拷貝
          啟動子: CMV
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 7703 bp
          5' 測序引物及序列: CMV-F:CGCAAATGGGCGGTAGGCGTG
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐
          備注: 本載體利用表達的嘌呤霉素作為篩選標記。嘌呤霉素和shRNA寡核苷酸被LoxP位點加在中間。Cre酶能夠同時使得這兩個基因在重組過程中切除出載體,同時啟動shRNA的表達。
          產品目錄號:  
          穩定性: /
          組成型: --
          病毒/非病毒: 慢病毒

          載體質粒圖譜和多克隆位點信息

          pSico PGK Puro載體圖譜

          載體簡介

          pSico PGK puro載體允許條件控制(通過Cre-LoxP),穩定表達shRNAs,能夠RNA干擾細胞和轉基因小鼠的基因表達。Cre序列的加入能夠使shRNA關閉表達。shRNA編碼寡核苷序列可以通過HpaI和XhoI限制性內切酶位點插入載體。

          載體序列

          LOCUS       pSico PGK puro	7703 bp 	DNA     circular	SYN
          DEFINITION  pSico PGK puro
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               source          1..7703
                               /organism="pSico PGK puro"
                               /mol_type="other DNA"
               promoter        239..815
                               /label="CMV_immearly_promoter"
               misc_feature    318..605
                               /label="CAG_enhancer"
               misc_feature    772..792
                               /label="CMV_fwd_primer"
               misc_feature    835..1015
                               /label="HIV-1_5_LTR"
               misc_feature    835..1015
                               /label="truncHIV-1_3_LTR"
               misc_feature    1126..1170
                               /label="HIV-1_psi_pack"
               CDS             1564..2451
                               /label="ORF frame 1"
                               /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                               FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                               QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                               INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                               VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                               GSALRAPILQTNGSIHPQF*"
               misc_feature    1686..1919
                               /label="RRE"
                               /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                               FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                               QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                               INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                               VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                               GSALRAPILQTNGSIHPQF*"
               misc_feature    2450..2465
                               /label="cPPT"
                               /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                               FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                               QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                               INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                               VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                               GSALRAPILQTNGSIHPQF*"
               misc_feature    complement(2891..2924)
                               /label="loxP"
                               /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                               FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                               QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                               INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                               VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                               GSALRAPILQTNGSIHPQF*"
               misc_feature    2891..2924
                               /label="loxP"
                               /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                               FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                               QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                               INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                               VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                               GSALRAPILQTNGSIHPQF*"
               misc_feature    complement(3001..3024)
                               /label="MSCV_rev_primer"
                               /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                               FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                               QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                               INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                               VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                               GSALRAPILQTNGSIHPQF*"
               CDS             complement(3356..4159)
                               /label="ORF frame 2"
                               /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                               PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                               RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                               DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                               KLQLLDRDLDGCRSKGPEMRKRRTARQTCAFEACRMPGLRRTFGRPPRP*"
               misc_feature    3398..3417
                               /label="mPGK_F_primer"
                               /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                               PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                               RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                               DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                               KLQLLDRDLDGCRSKGPEMRKRRTARQTCAFEACRMPGLRRTFGRPPRP*"
               gene            3509..4108
                               /label="puro (variant)"
                               /gene="puro (variant)"
                               /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                               PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                               RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                               DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                               KLQLLDRDLDGCRSKGPEMRKRRTARQTCAFEACRMPGLRRTFGRPPRP*"
               CDS             3509..4108
                               /label="ORF frame 2"
                               /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                               RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                               LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                               ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
               misc_feature    complement(4441..4474)
                               /label="loxP"
                               /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                               RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                               LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                               ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
               misc_feature    4441..4474
                               /label="loxP"
                               /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                               RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                               LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                               ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
               misc_feature    4500..4516
                               /label="pBluescriptKS_primer"
                               /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                               RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                               LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                               ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
               misc_feature    4594..5181
                               /label="WPRE"
                               /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                               RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                               LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                               ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
               CDS             4682..5236
                               /label="ORF frame 2"
                               /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                               ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                               SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                               LWAASPHRYRRPRSRPRKTWSNHK*"
               CDS             4695..5735
                               /label="ORF frame 3"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                               AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                               CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                               PDCTGSLWLDQI*"
               misc_feature    complement(5184..5200)
                               /label="pBluescriptKS_primer"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                               AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                               CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                               PDCTGSLWLDQI*"
               misc_feature    5372..5393
                               /label="U3PPT"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                               AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                               CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                               PDCTGSLWLDQI*"
               misc_feature    5372..5387
                               /label="cPPT"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                               AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                               CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                               PDCTGSLWLDQI*"
               misc_feature    5709..5889
                               /label="HIV-1_5_LTR"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                               AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                               CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                               PDCTGSLWLDQI*"
               misc_feature    5709..5889
                               /label="truncHIV-1_3_LTR"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                               AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                               CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                               PDCTGSLWLDQI*"
               gene            complement(6710..7570)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                               PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                               LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                               PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                               AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                               CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                               PDCTGSLWLDQI*"
               CDS             complement(6710..7570)
                               /label="ORF frame 2"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(7612..7640)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
             61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
            121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
            181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
            241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
            301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
            361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
            421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
            481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
            541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
            601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
            661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
            721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
            781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
            841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
            901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
            961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
           1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
           1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
           1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
           1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
           1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
           1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
           1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
           1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
           1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG GCCGCGCTGA TCTTCAGACC TGGAGGAGGA
           1561 GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA
           1621 TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG
           1681 GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG
           1741 TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC
           1801 AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC
           1861 AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG
           1921 GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT
           1981 TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA
           2041 GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA
           2101 GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT
           2161 AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA
           2221 GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA
           2281 CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA
           2341 GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCGGCA
           2401 CTGCGTGCGC CAATTCTGCA GACAAATGGC AGTATTCATC CACAATTTTA AAAGAAAAGG
           2461 GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC ATAATAGCAA CAGACATACA
           2521 AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT TTTCGGGTTT ATTACAGGGA
           2581 CAGCAGAGAT CCAGTTTGGT TAGTACCGGG CCCGCTCTAG AGATCCGACG CGCCATCTCT
           2641 AGGCCCGCGC CGGCCCCCTC GCACAGACTT GTGGGAGAAG CTCGGCTACT CCCCTGCCCC
           2701 GGTTAATTTG CATATAATAT TTCCTAGTAA CTATAGAGGC TTAATGTGCG ATAAAAGACA
           2761 GATAATCTGT TCTTTTTAAT ACTAGCTACA TTTTACATGA TAGGCTTGGA TTTCTATAAG
           2821 AGATACAAAT ACTAAATTAT TATTTTAAAA AACAGCACAA AAGGAAACTC ACCCTAACTG
           2881 TAAAGTAATT ATAACTTCGT ATAGTATAAA TTATACGAAG TTATAAGCCT TGGTTTTTTG
           2941 AATTCGTCGA CCTCGAAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA
           3001 GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC
           3061 ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG
           3121 CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC
           3181 AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT
           3241 GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT
           3301 TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG
           3361 GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC
           3421 ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC TTTCGACCTG CATCCATCTA
           3481 GATCTCGATC GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC
           3541 CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC
           3601 CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA
           3661 ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC
           3721 CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT
           3781 CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG
           3841 CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC
           3901 GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC
           3961 CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA
           4021 CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG
           4081 GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA
           4141 AAGGAGCGCA CGACCCCATG CATCGATGAT ATCATAATTT AAACAAGCAA AACCAAATTA
           4201 AGGGCCAGCT CATTCCTCCC ACTCATGATC TATAGATCTA TAGATCTCTC GTGGGATCAT
           4261 TGTTTTTCTC TTGATTCCCA CTTTGTGGTT CTAAGTACTG TGGTTTCCAA ATGTGTCAGT
           4321 TTCATAGCCT GAAGAACGAG ATCAGCAGCC TCTGTTCCAC ATACACTTCA TTCTCAGTAT
           4381 TGTTTTGCCA AGTTCTAATT CCATCAGAAG CTGGTCGACT CTAGCTAGAT GCGCGGCCGC
           4441 ATAACTTCGT ATAGTATAAA TTATACGAAG TTATAAGCCT TGTTAACGCG CGGTGACCCT
           4501 CGAGGTCGAC GGTATCGATA AGCTCGCTTC ACGAGATCAT GTTTAAGGGT TCCGGTTCCA
           4561 CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA
           4621 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT
           4681 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA
           4741 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT
           4801 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT
           4861 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG
           4921 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC
           4981 GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG
           5041 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT
           5101 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC
           5161 CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG
           5221 GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC
           5281 AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT
           5341 ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA
           5401 TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT
           5461 TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT
           5521 GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA
           5581 ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT
           5641 TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG
           5701 ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
           5761 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
           5821 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
           5881 TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
           5941 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
           6001 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
           6061 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
           6121 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
           6181 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
           6241 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
           6301 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
           6361 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA
           6421 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
           6481 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
           6541 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
           6601 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
           6661 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
           6721 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
           6781 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
           6841 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
           6901 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
           6961 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
           7021 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
           7081 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
           7141 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
           7201 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
           7261 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
           7321 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
           7381 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
           7441 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
           7501 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
           7561 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
           7621 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
           7681 TTCCCCGAAA AGTGCCACCT GAC
          //
          

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