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          pPRIME-TET-GFP-FF3

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Addgene
          載體名稱: pPRIME-TET-GFP-FF3
          質粒類型: 慢病毒載體;RNAi載體;四環素誘導載體
          高拷貝/低拷貝: 低拷貝
          啟動子: TET
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 8226bp
          5' 測序引物及序列: EGFP-C Forward: CATGGTCCTGCTGGAGTTCGTG
          3' 測序引物及序列: --
          載體標簽: GFP
          載體抗性: 氨芐青霉素
          篩選標記: 氯霉素
          備注: 克隆宿主菌為Stbl3,在30度條件下進行質粒擴增。
          產品目錄號: 11662
          穩定性: 穩表達
          組成型: 誘導型
          病毒/非病毒: 慢病毒

          載體質粒圖譜和多克隆位點信息

          pPRIME-TET-GFP-FF3載體圖譜

          載體簡介

          載體序列

          LOCUS       pPRIME-TET-GFP-FF3	8226 bp 	DNA	SYN
          DEFINITION  pPRIME-TET-GFP-FF3
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..8226
                               /organism="pPRIME-TET-GFP-FF3"
                               /mol_type="other DNA"
               promoter        239..803
                               /label="CMV_immearly_promoter"
               misc_feature    318..605
                               /label="CAG_enhancer"
               misc_feature    772..792
                               /label="CMV_fwd_primer"
               misc_feature    820..976
                               /label="HIV-1_5_LTR"
               misc_feature    820..976
                               /label="truncHIV-1_3_LTR"
               misc_feature    1087..1131
                               /label="HIV-1_psi_pack"
               CDS             1523..2410
                               /label="ORF frame 2"
          
               misc_feature    1645..1878
                               /label="RRE"
          
               misc_feature    2409..2424
                               /label="cPPT"
          
               CDS             2663..3700
                               /label="ORF frame 2"
          
               misc_feature    2882..2901
                               /label="pCEP_fwd_primer"
          
               misc_feature    2884..2908
                               /label="LNCX_primer"
          
               CDS             2928..3647
                               /label="ORF frame 3"
          
               gene            2931..3644
                               /label="EGFP"
                               /gene="EGFP"
          
               misc_feature    complement(2973..2994)
                               /label="EGFP_N_primer"
          
               misc_feature    3111..3140
                               /label="Y66 (EGFP)"
          
               misc_feature    3581..3602
                               /label="EGFP_C_primer"
          
               gene            4172..4831
                               /label="CAT/CamR"
                               /gene="CAT/CamR"
          
               CDS             4172..4831
                               /label="ORF frame 2"
          
               misc_feature    5117..5704
                               /label="WPRE"
          
               CDS             5205..5759
                               /label="ORF frame 3"
          
               CDS             5218..6258
                               /label="ORF frame 1"
          
               misc_feature    complement(5707..5723)
                               /label="pBluescriptKS_primer"
          
               misc_feature    5895..5910
                               /label="cPPT"
          
               misc_feature    5895..5916
                               /label="U3PPT"
          
               misc_feature    6232..6412
                               /label="HIV-1_5_LTR"
          
               misc_feature    6232..6412
                               /label="truncHIV-1_3_LTR"
          
               gene            complement(7233..8093)
                               /label="Ampicillin"
                               /gene="Ampicillin"
          
               CDS             complement(7233..8093)
                               /label="ORF frame 2"
          
               promoter        complement(8135..8163)
                               /label="AmpR_promoter"
          
          ORIGIN
              1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
             61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
            121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
            181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
            241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
            301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
            361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
            421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
            481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
            541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
            601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
            661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
            721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
            781 gcggtaggcg tgtacggtgg gaggtctata gaccagatct gagcctggga gctctctggc
            841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
            901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
            961 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
           1021 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
           1081 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
           1141 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
           1201 cagggggaaa gaaaaaatat aaataaacat atagtatggg caagcaggga gctagaacga
           1261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
           1321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
           1381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
           1441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat
           1501 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
           1561 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
           1621 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
           1681 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
           1741 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
           1801 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
           1861 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
           1921 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
           1981 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
           2041 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
           2101 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
           2161 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
           2221 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
           2281 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
           2341 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
           2401 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
           2461 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
           2521 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
           2581 cctttcgtct tcattcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
           2641 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
           2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
           2761 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
           2821 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
           2881 cagagctcgt ttagtgaacc gtcagatcgc ctggaccggt cgccaccatg gtgagcaagg
           2941 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
           3001 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
           3061 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
           3121 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
           3181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
           3241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
           3301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
           3361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
           3421 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
           3481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
           3541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
           3601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagcgg ccgcaagcct
           3661 tgttaagtgc tcgcttcggc agcacatata ctatgtttga atgaggcttc agtactttac
           3721 agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca
           3781 acagaaggct cgagcaacca gaattcaagg ggctacttta ggagcaatta tcttgtttac
           3841 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta
           3901 taaattaaat cacttttttc aattggaaga ctaatgcgtt taaacacgcg gcgacgcgtt
           3961 cgaccgaata aaacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc
           4021 tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt
           4081 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
           4141 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
           4201 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
           4261 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
           4321 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
           4381 tccggaatta cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
           4441 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
           4501 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
           4561 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
           4621 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
           4681 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
           4741 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
           4801 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
           4861 aacgcctggt tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgga
           4921 tctcgaccgc gtttgggcgg tggctccctg ccacgcggct ccgaacagaa gctgatctcc
           4981 gaagaggatc tgacatgtgt ttaaacctcg acttaattaa gtcgagggtc gacggtatcg
           5041 ataagctcgc ttcacgagat catgtttaag ggttccggtt ccactaggta caattcgata
           5101 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
           5161 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
           5221 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
           5281 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
           5341 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
           5401 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
           5461 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
           5521 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
           5581 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
           5641 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
           5701 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
           5761 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
           5821 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
           5881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
           5941 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
           6001 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
           6061 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
           6121 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
           6181 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
           6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
           6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
           6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
           6421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
           6481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
           6541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
           6601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
           6661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
           6721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
           6781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
           6841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
           6901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
           6961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
           7021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
           7081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
           7141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
           7201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
           7261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
           7321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
           7381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
           7441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
           7501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
           7561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
           7621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
           7681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
           7741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
           7801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
           7861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
           7921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
           7981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
           8041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
           8101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
           8161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
           8221 cctgac
          //
          

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