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          pLenti CMV/TO Hygro empty (w214-1)

          價格:1200元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          質粒名稱: pLenti CMV/TO Hygro empty (w214-1) 
          出品公司: Addgene
          目錄編號: 17484
          存儲實驗室: Eric Campeau

          載體骨架基本信息
          載體名稱: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
          載體抗性: 氨芐霉素
          空載體大小: 8709 bp
          載體類型: 哺乳細胞表達
          篩選標記: 潮霉素
          高拷貝/低拷貝: 高拷貝
          生長條件: 37 ℃

          插入基因信息
          克隆方法: 限制性內切酶
          5' 克隆位點: Bam HI(未破壞)
          3' 克隆位點: Xba I(未破壞)
          5' 測序引物 CMV-F
          3' 測序引物  

          文獻引用方法
          材料和方法部分: pLenti CMV/TO Hygro empty (w214-1) was a gift from Eric Campeau (Addgene plasmid # 17484)
          參考文獻部分: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394

          載體質粒圖譜和多克隆位點信息

          pLenti CMV/TO Hygro empty (w214-1)載體圖譜

          載體簡介

          載體序列

          LOCUS       pLenti CMV/TO Hygro empty (w214-1)	8709 bp 	DNA	SYN
          DEFINITION  pLenti CMV/TO Hygro empty (w214-1)
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..8709
                               /organism="pLenti CMV/TO Hygro empty (w214-1)"
                               /mol_type="other DNA"
               promoter        62..90
                               /label="AmpR_promoter"
               gene            132..992
                               /label="Ampicillin"
                               /gene="Ampicillin"
               CDS             132..992
                               /label="ORF frame 3"
               rep_origin      1147..1766
                               /label="pBR322_origin"
               promoter        2075..2104
                               /label="lac_promoter"
               misc_feature    2118..2140
                               /label="M13_pUC_rev_primer"
               promoter        2139..2157
                               /label="M13_reverse_primer"
               promoter        2174..2193
                               /label="T3_promoter"
               misc_feature    2448..2628
                               /label="HIV-1_5_LTR"
               misc_feature    2448..2628
                               /label="truncHIV-1_3_LTR"
               misc_feature    2736..2780
                               /label="HIV-1_psi_pack"
               CDS             3168..3668
                               /label="ORF frame 3"
               misc_feature    3290..3523
                               /label="RRE"
               CDS             3629..4171
                               /label="ORF frame 2"
               misc_feature    4016..4031
                               /label="cPPT"
               promoter        4149..4653
                               /label="CMV_immearly_promoter"
               misc_feature    4156..4443
                               /label="CAG_enhancer"
               misc_feature    4610..4630
                               /label="CMV_fwd_primer"
               misc_feature    4661..4700
                               /label="tetO"
               misc_feature    4741..4765
                               /label="attB1"
               misc_feature    4834..4858
                               /label="attB2"
               misc_feature    4891..5466
                               /label="WPRE"
               CDS             4979..5521
                               /label="ORF frame 2"
               misc_feature    complement(5568..5591)
                               /label="MSCV_rev_primer"
               CDS             5813..6865
                               /label="ORF frame 2"
               misc_feature    5965..5984
                               /label="mPGK_F_primer"
               CDS             complement(5978..6856)
                               /label="ORF frame 3"
               gene            6065..7073
                               /label="hygroB"
                               /gene="hygroB"
               misc_feature    7433..7454
                               /label="U3PPT"
               misc_feature    7433..7448
                               /label="cPPT"
               misc_feature    7503..7683
                               /label="truncHIV-1_3_LTR"
               misc_feature    7503..7683
                               /label="HIV-1_5_LTR"
               terminator      7758..7877
                               /label="SV40_PA_terminator"
               misc_feature    7846..7865
                               /label="EBV_rev_primer"
               rep_origin      7895..7972
                               /label="SV40_origin"
               misc_feature    7957..7976
                               /label="SV40pro_F_primer"
               promoter        complement(8063..8081)
                               /label="T7_promoter"
               promoter        complement(8091..8107)
                               /label="M13_forward20_primer"
               misc_feature    complement(8100..8122)
                               /label="M13_pUC_fwd_primer"
               misc_feature    8100..8243
                               /label="lacZ_a"
               rep_origin      8269..8575
                               /label="f1_origin"
          ORIGIN
              1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
             61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
            121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
            181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
            241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
            301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
            361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
            421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
            481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
            541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
            601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
            661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
            721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
            781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
            841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
            901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
            961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
           1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
           1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
           1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
           1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
           1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
           1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
           1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
           1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
           1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
           1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
           1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
           1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
           1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
           1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
           1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
           1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
           1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
           2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
           2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
           2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
           2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
           2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
           2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
           2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
           2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
           2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
           2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
           2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
           2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
           2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
           2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
           2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
           2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
           3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
           3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
           3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
           3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
           3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
           3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
           3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
           3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
           3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
           3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
           3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
           3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
           3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
           3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
           3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
           3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
           3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
           4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
           4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
           4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
           4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
           4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
           4321 tatCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
           4381 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
           4441 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
           4501 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
           4561 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
           4621 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCCCTATCAG TGATAGAGAT
           4681 CTCCCTATCA GTGATAGAGA TCGTCGACTA GTCCAGTGTG GTGGAATTCT GCAGATATCA
           4741 ACAAGTTTGT ACAAAAAAGC AGGCTCCACC ATGGGAACCA ATTCAGTCGA CTGGATCCGG
           4801 TACCGAATTC GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG
           4861 ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa tttgtgaaag
           4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
           4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
           5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
           5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
           5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
           5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
           5281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
           5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
           5401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
           5461 ttgggccgcc tccccgcctg gAATTCTGCA GATATCCGGA CTAGTGATCT CTCGAGGTTA
           5521 ACGAATTCTA CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG
           5581 CAGCCCCGCT GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT
           5641 CCACCGGTAG GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC
           5701 CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA
           5761 TGGAAGTAGC ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC
           5821 GGGTAGGCCT TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA
           5881 GGCTGGGAAG GGGTGGGTCC GGGGGCGGGC TCAGGGGCGG GCTCAGGGGC GGGGCGGGCG
           5941 CCCGAAGGTC CTCCGGAGGC CCGGCATTCT GCACGCTTCA AAAGCGCACG TCTGCCGCGC
           6001 TGTTCTCCTC TTCCTCATCT CCGGGCCTTT CGACCTGCAT CCCGCCACCA TGAAAAAGCC
           6061 TGAACTCACC GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA
           6121 CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG
           6181 TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA
           6241 TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG
           6301 CGAGAGCCTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC
           6361 TGAAACCGAA CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC
           6421 GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA
           6481 CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC
           6541 TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG
           6601 GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT
           6661 CCTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA
           6721 TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA
           6781 GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGG
           6841 CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG
           6901 ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG
           6961 TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG
           7021 TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG GAATAGAGTA
           7081 GATGCCGACC GAACAAGAGC TGATTTCGAG AACGCCTCAG CCAGCAACTC GCGCGAGCCT
           7141 AGCAAGGCAA ATGCGAGAGA ACGGCCTTAC GCTTGGTGGC ACAGTTCTCG TCCACAGTTC
           7201 GCTAAGCTCG CTCGGCTGGG TCGCGGGAGG GCCGGTCGCA GTGATTCAGG CCCTTCTGGA
           7261 TTGTGTTGGT CCCCAGGGCA CGATTGTCAT GCCCACGCAC TCGGGTGATC TGACTGATCC
           7321 CGCAGATTGG AGATCGCCGC CCGTGCCTGC CGATTGGGTG CAGATCCGTC GAGGGCCCGG
           7381 TACctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
           7441 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
           7501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctgc ctagggaacc
           7561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
           7621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
           7681 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
           7741 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
           7801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
           7861 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
           7921 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
           7981 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
           8041 cgtcgagacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
           8101 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
           8161 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
           8221 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg
           8281 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
           8341 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
           8401 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
           8461 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
           8521 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
           8581 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
           8641 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
           8701 acaatttcc
          //
          

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