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          pLVX-EF1α-IRES-ZsGreen1

          價格:2500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Clontech
          載體名稱: pLVX-EF1α-IRES-ZsGreen1, pLVX-EF1a-IRES-ZsGreen1, pLVX-EF1alpha-IRES-ZsGreen1
          質粒類型: 哺乳動物細胞慢病毒表達載體
          高拷貝/低拷貝: 高拷貝
          啟動子: EF1α/ EF1a
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 8889 bp
          5' 測序引物及序列: EF1a Forward: TCAAGCCTCAGACAGTGGTTC
          3' 測序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: ZsGreen1
          備注: 克隆宿主菌株 Stbl3
          產品目錄號: 631982
          穩定性: 穩表達
          組成型: 組成型
          病毒/非病毒: 慢病毒

          載體質粒圖譜和多克隆位點信息

          pLVX-EF1α-IRES-ZsGreen1載體圖譜



          pLVX-EF1α-IRES-ZsGreen1多克隆位點

          pLVX-EF1α-IRES-ZsGreen1載體特征

          載體簡介

          載體序列

          LOCUS       pLVX-EF1a-IRES-ZsGreen1   8889 bp    DNA   circular            11-FEB-2010
          COMMENT     Created by Clontech Laboratories Inc.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          COMMENT     Cat. No. 631982
          FEATURES             Location/Qualifiers
               CDS             4149..4844
                               /label=ZsGreen1
               misc_feature    3574..4148
                               /label=IRES
               LTR             1..635
                               /label=5'\LTR
               misc_feature    685..822
                               /label=Packaging\signal
               misc_feature    2028..2151
                               /label=cPPT/CTS\(DNA\flap)
               misc_feature    4858..5449
                               /label=WPRE
               rep_origin      complement(6757..7430)
                               /label=pUC\origin
               CDS             complement(7575..8571)
                               /label=Amp(R)
               misc_binding    1303..1536
                               /label=RRE
               misc_binding    636..653
                               /label=PBS
               LTR             5652..6288
                               /label=3'\LTR
               misc_feature    3535..3572
                               /label=MCS
               promoter        2186..3519
                               /label=EF1a\Promoter
          BASE COUNT     2190 a      2213 c      2366 g      2120 t 
          ORIGIN
                  1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
                 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
                121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
                181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
                241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
                301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
                361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
                421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
                481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
                541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
                601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
                661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
                721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
                781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
                841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
                901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
                961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
               1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
               1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
               1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
               1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
               1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
               1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
               1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
               1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
               1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
               1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
               1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
               1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
               1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
               1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
               1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
               1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
               1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
               2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
               2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
               2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
               2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
               2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
               2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
               2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
               2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
               2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
               2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
               2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
               2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
               2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
               2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
               2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
               2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
               3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
               3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
               3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
               3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
               3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
               3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
               3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
               3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
               3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 
               3541 ctcgagacta gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg 
               3601 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 
               3661 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 
               3721 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 
               3781 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 
               3841 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 
               3901 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 
               3961 gagtcaaatg gctcacctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 
               4021 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 
               4081 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg 
               4141 atgataatat ggcccagtcc aagcacggcc tgaccaagga gatgaccatg aagtaccgca 
               4201 tggagggctg cgtggacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct 
               4261 tcaagggcaa gcaggccatc aacctgtgcg tggtggaggg cggccccttg cccttcgccg 
               4321 aggacatctt gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag tacccccagg 
               4381 acatcgtcga ctacttcaag aactcctgcc ccgccggcta cacctgggac cgctccttcc 
               4441 tgttcgagga cggcgccgtg tgcatctgca acgccgacat caccgtgagc gtggaggaga 
               4501 actgcatgta ccacgagtcc aagttctacg gcgtgaactt ccccgccgac ggccccgtga 
               4561 tgaagaagat gaccgacaac tgggagccct cctgcgagaa gatcatcccc gtgcccaagc 
               4621 agggcatctt gaagggcgac gtgagcatgt acctgctgct gaaggacggt ggccgcttgc 
               4681 gctgccagtt cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg cccgactggc 
               4741 acttcatcca gcacaagctg acccgcgagg accgcagcga cgccaagaac cagaagtggc 
               4801 acctgaccga gcacgccatc gcctccggct ccgccttgcc ctgaacgcgt ctggaacaat 
               4861 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
               4921 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
               4981 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 
               5041 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 
               5101 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 
               5161 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 
               5221 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 
               5281 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 
               5341 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 
               5401 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct 
               5461 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 
               5521 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 
               5581 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 
               5641 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 
               5701 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 
               5761 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 
               5821 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 
               5881 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 
               5941 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 
               6001 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 
               6061 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 
               6121 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
               6181 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
               6241 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 
               6301 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 
               6361 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag 
               6421 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
               6481 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
               6541 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
               6601 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
               6661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
               6721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
               6781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
               6841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
               6901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
               6961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
               7021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
               7081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
               7141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
               7201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 
               7261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
               7321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
               7381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
               7441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
               7501 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
               7561 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
               7621 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
               7681 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
               7741 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
               7801 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
               7861 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
               7921 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
               7981 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
               8041 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
               8101 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
               8161 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
               8221 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
               8281 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
               8341 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
               8401 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
               8461 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
               8521 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 
               8581 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
               8641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
               8701 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact 
               8761 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg 
               8821 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa 
               8881 cttagtagt 
          //
          

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