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          pLVX-EF1α-IRES-Puro

          價格:2500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Clontech
          載體名稱: pLVX-EF1α-IRES-Puro,  pLVX-EF1a-IRES-Puro,  pLVX-EF1alpha-IRES-Puro
          質粒類型: 哺乳動物細胞慢病毒表達載體
          高拷貝/低拷貝: 高拷貝
          啟動子: EF1α/ EF1a
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 8825 bp
          5' 測序引物及序列: EF-1a Forward: TCAAGCCTCAGACAGTGGTTC
          3' 測序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: 嘌呤霉素(Puromycin)
          克隆宿主菌: Stbl3
          備注: --
          產品目錄號: 631988
          穩定性: 穩表達
          組成型: 組成型
          病毒/非病毒: 慢病毒

          載體質粒圖譜和多克隆位點信息

          pLVX-EF1α-IRES-Puro載體圖譜



          pLVX-EF1α-IRES-Puro多克隆位點

          pLVX-EF1α-IRES-Puro載體特征

          載體簡介

          載體序列

          LOCUS       pLVX-EF1a-IRES-Puro   8825 bp    DNA   circular            12-FEB-2010
          COMMENT     Created by Clontech Laboratories Inc. 
          COMMENT     Cat. No. 631988,631253
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               LTR             1..635
                               /label=5'\LTR
               misc_feature    685..822
                               /label=Packaging\signal
               misc_feature    2028..2151
                               /label=cPPT/CTS\(DNA\flap)
               misc_feature    4794..5385
                               /label=WPRE
               rep_origin      complement(6693..7366)
                               /label=pUC\origin
               CDS             complement(7511..8507)
                               /label=Amp(R)
               misc_binding    1303..1536
                               /label=RRE
               misc_binding    636..653
                               /label=PBS
               LTR             5588..6224
                               /label=LTR
               misc_feature    3573..4148
                               /label=IRES
               CDS             4181..4780
                               /label=Puro(R)
               promoter        2185..3519
                               /label=EF1a\Promoter
               misc_feature    3535..3572
                               /label=MCS
          BASE COUNT     2140 a      2225 c      2374 g      2086 t 
          ORIGIN
                  1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
                 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
                121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
                181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
                241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
                301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
                361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
                421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
                481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
                541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
                601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
                661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
                721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
                781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
                841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
                901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
                961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
               1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
               1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
               1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
               1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
               1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
               1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
               1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
               1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
               1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
               1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
               1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
               1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
               1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
               1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
               1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
               1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
               1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
               2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
               2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
               2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
               2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
               2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
               2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
               2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
               2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
               2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
               2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
               2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
               2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
               2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
               2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
               2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
               2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
               3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
               3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
               3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
               3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
               3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
               3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
               3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
               3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
               3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 
               3541 ctcgagacta gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg 
               3601 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 
               3661 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 
               3721 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 
               3781 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 
               3841 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 
               3901 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 
               3961 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 
               4021 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 
               4081 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 
               4141 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac 
               4201 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt 
               4261 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac 
               4321 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
               4381 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
               4441 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
               4501 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
               4561 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
               4621 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
               4681 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 
               4741 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac 
               4801 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
               4861 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
               4921 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
               4981 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
               5041 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
               5101 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
               5161 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 
               5221 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
               5281 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
               5341 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag 
               5401 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga 
               5461 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt 
               5521 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
               5581 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat 
               5641 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag 
               5701 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga 
               5761 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga 
               5821 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt 
               5881 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg 
               5941 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc 
               6001 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 
               6061 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
               6121 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
               6181 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 
               6241 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac 
               6301 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
               6361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
               6421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
               6481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
               6541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
               6601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
               6661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
               6721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
               6781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
               6841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
               6901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
               6961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
               7021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
               7081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
               7141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
               7201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
               7261 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
               7321 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
               7381 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
               7441 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
               7501 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
               7561 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
               7621 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
               7681 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
               7741 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
               7801 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
               7861 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
               7921 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
               7981 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
               8041 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
               8101 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
               8161 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
               8221 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
               8281 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
               8341 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
               8401 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
               8461 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 
               8521 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
               8581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
               8641 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 
               8701 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 
               8761 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 
               8821 gtagt 
          //
          

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