免费高清视频 成人免费视频 成年在线视频免费观看视频

    <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

      1. <pre id="q7rs8"></pre>
        <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
        <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

          pLVX-EF1α-DsRed-Monomer-C1

          價格:1350元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Clontech
          載體名稱: pLVX-EF1α-DsRed-Monomer-C1, pLVX-EF1a-DsRed-Monomer-C1, pLVX-EF1alpha-DsRed-Monomer-C1
          質粒類型: 慢病毒表達載體;熒光報告載體
          高拷貝/低拷貝: 高拷貝
          啟動子: EF1α
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 9485 bp
          5' 測序引物及序列: EF1a Forward: TCAAGCCTCAGACAGTGGTTC
          3' 測序引物及序列: --
          載體標簽: N-DsRed-Monomer
          載體抗性: 氨芐青霉素
          篩選標記: 嘌呤霉素(Puromycin)
          克隆菌株: Stbl3
          宿主細胞(系): 常規細胞系,293、CV-1、CHO等
          備注: pLVX-EF1α-DsRed-Monomer-C1載體能夠表達N端DsRed Monomer融合蛋白;
          DsRed-Monomer是DsRed的單體突變體,與DsRed相比,編碼序列經過了人工優化,適用于在哺乳動物細胞中的高水平表達;
          EF1α啟動子是高表達啟動子,對于那些使用CMV啟動子表達效果不理想的細胞系具有良好效果。
          產品目錄號: 631989
          穩定性: 穩表達
          組成型: 組成型
          病毒/非病毒: 慢病毒

          載體質粒圖譜和多克隆位點信息

          pLVX-EF1α-DsRed-Monomer-C1載體圖譜



          pLVX-EF1α-DsRed-Monomer-C1多克隆位點

          pLVX-EF1α-DsRed-Monomer-C1載體特征

          載體簡介

          載體序列

          LOCUS       pLVX-EF1a-DsRed-Monomer-C1   9485 bp    DNA   circular            22-MAR-2010
          COMMENT     Created by Clontech Laboratories Inc.
          COMMENT     Cat. No. 631989
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               LTR             1..635
                               /label=5'\LTR
               misc_feature    685..822
                               /label=Packaging\signal
               misc_feature    2028..2151
                               /label=cPPT/CTS\(DNA\flap)
               misc_feature    5453..6044
                               /label=WPRE
               rep_origin      complement(7353..8026)
                               /label=pUC\origin
               CDS             complement(8171..9167)
                               /label=Amp(R)
               misc_binding    1303..1536
                               /label=RRE
               misc_binding    636..653
                               /label=PBS
               LTR             6247..6883
                               /label=LTR
               misc_feature    3547..4221
                               /label=DsRed\Monomer
               misc_feature    4296..4298
                               /label=Stop
               misc_feature    4300..4302
                               /label=Stop
               misc_feature    4304..4306
                               /label=Stop
               misc_feature    4235..4288
                               /label=MCS
               misc_feature    4311..4819
                               /label=PGK\Promoter
               misc_feature    4840..5439
                               /label=Puro(R)
               promoter        2185..3519
                               /label=EF1a\Promoter
          BASE COUNT     2246 a      2484 c      2600 g      2155 t 
          ORIGIN
                  1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
                 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
                121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
                181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
                241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
                301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
                361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
                421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
                481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
                541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
                601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
                661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
                721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
                781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
                841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
                901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
                961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
               1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
               1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
               1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
               1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
               1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
               1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
               1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
               1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
               1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
               1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
               1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
               1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
               1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
               1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
               1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
               1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
               1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
               2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
               2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
               2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
               2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
               2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
               2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
               2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
               2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
               2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
               2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
               2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
               2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
               2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
               2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
               2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
               2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
               3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
               3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
               3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
               3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
               3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
               3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
               3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
               3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
               3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggtc 
               3541 gccaccatgg acaacaccga ggacgtcatc aaggagttca tgcagttcaa ggtgcgcatg 
               3601 gagggctccg tgaacggcca ctacttcgag atcgagggcg agggcgaggg caagccctac 
               3661 gagggcaccc agaccgccaa gctgcaggtg accaagggcg gccccctgcc cttcgcctgg 
               3721 gacatcctgt ccccccagtt ccagtacggc tccaaggcct acgtgaagca ccccgccgac 
               3781 atccccgact acatgaagct gtccttcccc gagggcttca cctgggagcg ctccatgaac 
               3841 ttcgaggacg gcggcgtggt ggaggtgcag caggactcct ccctgcagga cggcaccttc 
               3901 atctacaagg tgaagttcaa gggcgtgaac ttccccgccg acggccccgt aatgcagaag 
               3961 aagactgccg gctgggagcc ctccaccgag aagctgtacc cccaggacgg cgtgctgaag 
               4021 ggcgagatct cccacgccct gaagctgaag gacggcggcc actacacctg cgacttcaag 
               4081 accgtgtaca aggccaagaa gcccgtgcag ctgcccggca accactacgt ggactccaag 
               4141 ctggacatca ccaaccacaa cgaggactac accgtggtgg agcagtacga gcacgccgag 
               4201 gcccgccact ccggctccca gtccggactc agatctcgag ctcaagcttc gaattctgca 
               4261 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aattctaccg 
               4321 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 
               4381 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 
               4441 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 
               4501 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 
               4561 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 
               4621 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 
               4681 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 
               4741 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 
               4801 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 
               4861 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 
               4921 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 
               4981 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 
               5041 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 
               5101 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 
               5161 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 
               5221 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 
               5281 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 
               5341 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 
               5401 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 
               5461 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
               5521 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
               5581 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
               5641 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
               5701 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
               5761 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
               5821 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 
               5881 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
               5941 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
               6001 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 
               6061 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 
               6121 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 
               6181 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 
               6241 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 
               6301 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 
               6361 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 
               6421 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 
               6481 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 
               6541 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 
               6601 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 
               6661 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 
               6721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 
               6781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 
               6841 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 
               6901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 
               6961 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
               7021 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
               7081 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
               7141 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
               7201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
               7261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
               7321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
               7381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
               7441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
               7501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
               7561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
               7621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
               7681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
               7741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
               7801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
               7861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
               7921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
               7981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
               8041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
               8101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
               8161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
               8221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
               8281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
               8341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
               8401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
               8461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
               8521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
               8581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
               8641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
               8701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
               8761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
               8821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
               8881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
               8941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
               9001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
               9061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
               9121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 
               9181 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
               9241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
               9301 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 
               9361 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 
               9421 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 
               9481 gtagt 
          //
          

          載體質粒圖譜pdf版和相關資料下載

          載體應用舉例

          分享到:

          全部載體分類

          相關載體

          相關文章

          相關問答

          Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng
          歡迎您的訪問和咨詢....
            <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

              1. <pre id="q7rs8"></pre>
                <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
                <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

                  免费高清视频 成人免费视频 成年在线视频免费观看视频

                  品牌简介

                  免费高清视频 成人免费视频 成年在线视频免费观看视频