免费高清视频 成人免费视频 成年在线视频免费观看视频

    <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

      1. <pre id="q7rs8"></pre>
        <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
        <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

          pIB/V5-His-TOPO

          價格:2000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Invitrogen
          載體名稱: pIB/V5-His-TOPO
          質粒類型: 昆蟲表達載體;TOPO-TA克隆載體
          克隆方法: TOPO-TA
          啟動子: OplE2
          載體大小: 3537bp
          5' 測序引物及序列: OplE2 Forward:5'd[CGCAACGATCTGGTAAACAC]3'
          3' 測序引物及序列: --
          載體標簽: V5 Epitope Tag, His Tag (C-末端)
          載體抗性: 氨芐青霉素
          真核篩選標記: Blasticidin
          克隆菌株: TOP10
          宿主細胞(系): Sf9,Sf21,HighFive
          備注: --
          產品目錄號: K89020
          瞬表達/穩表達: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pIB-V5-His-TOPO 載體圖譜



          pIB-V5-His-TOPO 多克隆位點

          pIB-V5-His-TOPO 載體特征

          載體簡介

          描述
          The pIB/V5-His TOPO® TA Expression Kit offers five-minute cloning of Taq-amplified PCR products directly into the pIB/V5-His-TOPO expression vector. In addition to being TOPO Cloning ready, pIB/V5-His-TOPO includes:
          
          • The OpIE2 promoter for constitutive expression
          • The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
          • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
          
          表達水平
          The OpIE2 promoter provides relatively high levels of constitutive expression, although not all proteins will express as high as might be expected from baculovirus late promoters such as polyhedrin or very late promoters such as p10 (Jarvis et al., 1996).
          However, some researchers have found that the InsectSelect™ System expresses some proteins better than baculovirus systems. To date, reported expression levels range from 1-2 µg/ml (human IL-6; Invitrogen) to 8-10 µg/ml (human melanotransferrin) (Hegeduset al., 1999). 
          

          載體序列

          LOCUS       pIB/V5-His-TOPO	3537 bp 	DNA	SYN
          DEFINITION  pIB/V5-His-TOPO
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..3537
                               /organism="pIB/V5-His-TOPO"
                               /mol_type="other DNA"
               misc_feature    511..530
                               /label="OpIE2_fwd_primer"
               rep_origin      complement(1003..1622)
                               /label="pBR322_origin"
               promoter        1996..2063
                               /label="EM7_promoter"
               gene            2064..2462
                               /label="blast (variant)"
                               /gene="blast (variant)"
               gene            2582..3442
                               /label="Ampicillin"
                               /gene="Ampicillin"
               CDS             2582..3442
                               /label="ORF frame 2"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG
             61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT
            121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC
            181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC
            241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC
            301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT
            361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA
            421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC
            481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG
            541 CATCTGTTCG AATTTAAAGC TTGGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA
            601 TTGCCCTTAA GGGCAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG AGTCTAGAGG
            661 GCCCGCGGTT CGAAGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGCGTA
            721 CCGGTCATCA TCACCATCAC CATTGAGTTT ATCTGACTAA ATCTTAGTTT GTATTGTCAT
            781 GTTTTAATAC AATATGTTAT GTTTAAATAT GTTTTTAATA AATTTTATAA AATAATTTCA
            841 ACTTTTATTG TAACAACATT GTCCATTTAC ACACTCCTTT CAAGCGCGTG GGATCGATGC
            901 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
            961 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
           1021 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
           1081 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
           1141 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
           1201 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
           1261 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
           1321 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
           1381 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
           1441 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
           1501 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
           1561 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
           1621 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
           1681 GCGAAACACG CACGGCGCGC GCACGCAGCT TAGCACAAAC GCGTCGTTGC ACGCGCCCAC
           1741 CGCTAACCGC AGGCCAATCG GTCGGCCGGC CTCATATCCG CTCACCAGCC GCGTCCTATC
           1801 GGGCGCGGCT TCCGCGCCCA TTTTGAATAA ATAAACGATA ACGCCGTTGG TGGCGTGAGG
           1861 CATGTAAAAG GTTACATCAT TATCTTGTTC GCCATCCGGT TGGTATAAAT AGACGTTCAT
           1921 GTTGGTTTTT GTTTCAGTTG CAAGTTGGCT GCGGCGCGCG CAGCACCTTT GCCGGGATCT
           1981 GCCGGGCTGC AGCACGTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT
           2041 ACGACAAGGT GAGGAACTAA ACCATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA
           2101 TTGAAAGAGC AACGGCTACA ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA
           2161 GCGCAGCTCT CTCTAGCGAC GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG
           2221 GGGGACCTTG CGCAGAACTC GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC
           2281 TGACTTGTAT CGTCGCGATC GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT
           2341 GCCGACAGGT TCTTCTCGAT CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG
           2401 GACAGCCGAC GGCAGTTGGG ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT
           2461 AAGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTCCCGG GAGATCTGCA TGTCTACTAA
           2521 ACTCACAAAT TAGAGCTTCA ATTTAATTAT ATCAGTTATT ACCCATTGAA AAAGGAAGAG
           2581 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC
           2641 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC
           2701 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC
           2761 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC
           2821 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT
           2881 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT
           2941 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT
           3001 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT
           3061 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT
           3121 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC
           3181 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG
           3241 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC
           3301 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA
           3361 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC
           3421 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA
           3481 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCT
          
          //
          

          載體質粒圖譜pdf版和相關資料下載

          載體應用舉例

          分享到:

          全部載體分類

          相關載體

          相關文章

          相關問答

          Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng
          歡迎您的訪問和咨詢....
            <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

              1. <pre id="q7rs8"></pre>
                <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
                <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

                  免费高清视频 成人免费视频 成年在线视频免费观看视频

                  品牌简介

                  免费高清视频 成人免费视频 成年在线视频免费观看视频