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          pFastBac Dual

          價格:900元

          聯系方式:I47-825O-882O

          買家導航

          pFastBacDual載體基本信息

          出品公司: Invitrogen
          載體名稱: pFastBacDual, pFastBac Dual
          質粒類型: 昆蟲細胞表達載體
          啟動子: Polyhedrin, P10
          表達水平:
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 5238 bp
          5' 測序引物: pFastbac-F: TATTCCGGATTATTCATACC
          3' 測序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
          載體標簽: N-His, N-TEV
          載體抗性: 氨芐
          篩選標記: 慶大霉素
          備注: 在昆蟲細胞中,能夠同時表達兩個基因。
          產品目錄號: 10584-027
          穩定性: 穩表達 Stable
          組成型: 組成型 Constitutive
          病毒/非病毒: 桿狀病毒

          pFastBacDual載體質粒圖譜和多克隆位點信息

          pFastBacDual載體圖譜

          pFastBacDual多克隆位點

          pFastBacDual多克隆位點

          pFastBacDual載體簡介

          pFastBac Dual昆蟲細胞表達載體包含有兩個啟動子,能夠同時表達兩個外源基因,一個表達基因是polyhedrin (PH)啟動子控制,另一個外源基因是由p10啟動子控制。

          pFastBacDual載體序列

          LOCUS       pFastBac\Dual           5238 bp    DNA     circular SYN 16-MAY-2012
          DEFINITION  pFastBac Dual.
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               source          1..5238
                               /organism="pFastBac Dual"
                               /mol_type="other DNA"
                               /vntifkey="98"
               rep_origin      102..557
                               /vntifkey="33"
                               /label=f1_origin
               promoter        619..647
                               /vntifkey="30"
                               /label=AmpR_promoter
               CDS             689..1549
                               /gene="Ampicillin"
                               /vntifkey="4"
                               /label=Ampicillin
                               /note="ORF frame 2"
               gene            689..1549
                               /gene="Ampicillin"
                               /vntifkey="60"
               rep_origin      1704..2323
                               /vntifkey="33"
                               /label=pUC_origin
               gene            complement(2902..3435)
                               /gene="gent"
                               /vntifkey="60"
               terminator      complement(3996..4266)
                               /vntifkey="43"
                               /label=TK_PA_terminator
               misc_feature    4494..4511
                               /vntifkey="21"
                               /label=polyhedrin_fwd_primer
               promoter        4478..4606
                               /vntifkey="30"
                               /label=PH_promoter
               terminator      4831..4962
                               /vntifkey="43"
                               /label=SV40_PA_terminator
               misc_feature    4919..4938
                               /vntifkey="21"
                               /label=EBV_rev_primer
               misc_feature    2611..2835
                               /vntifkey="21"
                               /label=Tn7R
               CDS             complement(2902..3435)
                               /vntifkey="4"
                               /label=Gentimicin\Resistance\Gene
               misc_feature    complement(4274..4337)
                               /vntifkey="21"
                               /label=MCS
               misc_feature    4606..4704
                               /vntifkey="21"
                               /label=MCS
               promoter        complement(4338..4459)
                               /vntifkey="30"
                               /label=P10\Promoter
               misc_feature    complement(4991..5156)
                               /vntifkey="21"
                               /label=Tn7L
               misc_signal     complement(3992..4274)
                               /vntifkey="87"
                               /label=HSV\TK\polyadenylation\signal
               misc_signal     4722..4962
                               /vntifkey="87"
                               /label=SV40\polyadenylation\signal
          BASE COUNT     1294 a      1282 c      1259 g      1403 t
          ORIGIN
                  1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
                 61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
                121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
                181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
                241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
                301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
                361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
                421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
                481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
                541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
                601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
                661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
                721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
                781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
                841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
                901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
                961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
               1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
               1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
               1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
               1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
               1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
               1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
               1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
               1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
               1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
               1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
               1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
               1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
               1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
               1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
               1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
               1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
               1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
               2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
               2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
               2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
               2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
               2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
               2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
               2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
               2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
               2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
               2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
               2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
               2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
               2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
               2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
               2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
               2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
               3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
               3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
               3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
               3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
               3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
               3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
               3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
               3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
               3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
               3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
               3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
               3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
               3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
               3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
               3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
               3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
               3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
               4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
               4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
               4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
               4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
               4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
               4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
               4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
               4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
               4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
               4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa
               4621 gcgcgcggaa ttcaaaggcc tacgtcgacg agctcactag tcgcggccgc tttcgaatct
               4681 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc
               4741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
               4801 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
               4861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
               4921 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
               4981 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
               5041 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
               5101 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
               5161 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
               5221 gtcatcttcg gctacttt
          //
          

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