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          pFBDM

          價格:1250元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Invitrogen
          載體名稱: pFBDM
          質粒類型: 昆蟲細胞表達系統載體
          高拷貝/低拷貝: 高拷貝
          啟動子:
          Polyhedrin,p10
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 5279bp
          5' 測序引物及序列:
          p2Bac-F (for MCS1):ggacctttaattcaacccaacac
          Polyhedrin-F (for MCS2):AAATGATAACCATCTCGC
           
          3' 測序引物及序列:
          TK-pA-R (for MCS1):TTGTCTCCTTCCGTGTTTCA
          SV40pA-R (for MCS2):GAAATTTGTGATGCTATTGC
           
          Genbank: DJ417502.1
          載體標簽:
          載體抗性: 氨芐青霉素
          篩選標記: 慶大霉素
          備注: 可同時表達兩種蛋白
          產品目錄號: --
          穩定性: 穩定
          組成型: 非組成型
          病毒/非病毒: 桿狀病毒

          載體質粒圖譜和多克隆位點信息

          pFBDM載體圖譜



          pFBDM多克隆位點

          載體簡介

          載體序列

          LOCUS       pFBDM	5279 bp 	DNA   circular	SYN
          DEFINITION  pFBDM
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               source          1..5279
                               /organism="pFBDM"
                               /mol_type="other DNA"
               rep_origin      119..425
                               /label="f1_origin"
               promoter        619..647
                               /label="AmpR_promoter"
               gene            689..1549
                               /label="Ampicillin"
                               /gene="Ampicillin"
               CDS             689..1549
                               /label="ORF frame 2"
               rep_origin      1704..2323
                               /label="pBR322_origin"
               gene            complement(2902..3435)
                               /label="gent"
                               /gene="gent"
               CDS             complement(2902..3435)
                               /label="ORF frame 3"
               CDS             3537..4235
                               /label="ORF frame 3"
               terminator      complement(4016..4286)
                               /label="TK_PA_terminator"
               promoter        4521..4609
                               /label="PH_promoter"
               misc_feature    4535..4552
                               /label="polyhedrin_fwd_primer"
               terminator      4872..5003
                               /label="SV40_PA_terminator"
               misc_feature    4960..4979
                               /label="EBV_rev_primer"
          ORIGIN
              1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
             61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
            121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
            181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
            241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
            301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
            361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
            421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
            481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
            541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
            601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
            661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
            721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
            781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
            841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
            901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
            961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
           1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
           1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
           1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
           1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
           1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
           1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
           1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
           1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
           1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
           1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
           1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
           1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
           1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
           1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
           1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
           1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
           1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
           2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
           2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
           2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
           2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
           2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
           2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
           2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
           2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
           2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
           2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
           2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
           2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
           2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
           2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
           2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
           2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
           3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
           3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
           3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
           3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
           3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
           3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
           3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
           3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
           3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
           3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
           3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
           3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
           3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
           3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
           3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
           3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
           3961 atagttctag tggttggcct acagctttgt ttaaacaaag ctgtacccgt agtggctatg
           4021 gcagggcttg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggt
           4081 tggggtgggg aaaaggaaga aacgcgggcg tattggtccc aatggggtct cggtggggta
           4141 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc
           4201 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
           4261 cttccgtgtt tcagttagcc tcccccatct cccggtaccg catgctatgc atcagctgct
           4321 agcaccatgg ctcgagatcc cgggtgatca agtcttcgtc gagtgattgt aaataaaatg
           4381 taatttacag tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa
           4441 ctataatata ttgtgttggg ttgaattaaa ggtccgtata ctagtatcga ttcgcgacct
           4501 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
           4561 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
           4621 attcataccg tcccaccatc gggcgcggat cccggtccga agcgcgcgga attcaaaggc
           4681 ctacgtcgac gagctcacta gtcgcggccg ctttcgaatc tagagcctgc agtctcgaca
           4741 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
           4801 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
           4861 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
           4921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
           4981 atgtatctta tcatgtctgg atctgatcac tgcttgagcc taggagatcc gaaccagata
           5041 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
           5101 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
           5161 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
           5221 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctacttt
          
          //
          

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