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          pAcGHLT C

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          pAcGHLTC載體基本信息

          出品公司: BD Biosciences
          載體名稱: pAcGHLT C
          質粒類型: 昆蟲細胞載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: --
          啟動子: --
          載體大小: 8753 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: --
          克隆菌株: --
          宿主細胞(系): --
          備注: --
          產品目錄號: --
          穩定性: --
          組成型/誘導型: --
          病毒/非病毒: --

          pAcGHLTC載體質粒圖譜和多克隆位點信息

          pAcGHLT C載體圖譜

          pAcGHLTC載體簡介

          pAcGHLTC載體序列

          LOCUS       pAcGHLT C                8753 bp ds-DNA     circular SYN 19-APR-2014
          DEFINITION  Baculovirus transfer vector for expressing proteins with an 
                      N-terminal GST-6xHis-PKA-thrombin cassette. For other reading 
                      frames, use pAcGHLT A or pAcGHLT B.
          ACCESSION   .
          VERSION     .
          KEYWORDS    pAcGHLT C
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 8753)
            AUTHORS   BD Biosciences
            TITLE     Direct Submission
            JOURNAL   Exported Thursday, February 6, 2020 from SnapGene Viewer 3.1.4
                      http://www.snapgene.com
          FEATURES             Location/Qualifiers
               source          1..8753
                               /organism="synthetic DNA construct"
                               /lab_host="Insect Cells"
                               /mol_type="other DNA"
               misc_recomb     1269..2096
                               /note="baculovirus recombination region (lef2/ORF603)"
                               /note="contains ORF603 and part of lef2"
               CDS             1269..1393
                               /codon_start=3
                               /product="baculovirus late expression factor 2"
                               /note="lef2"
                               /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
               CDS             complement(1431..2036)
                               /codon_start=1
                               /product="baculovirus ORF603 protein"
                               /note="ORF603"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                               IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                               KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                               INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
               promoter        2100..2191
                               /gene="polh from Autographa californica"
                               /note="polyhedrin promoter"
                               /note="promoter for the baculovirus polyhedrin gene"
               CDS             2206..2859
                               /codon_start=1
                               /product="glutathione S-transferase from Schistosoma 
                               japonicum"
                               /note="GST"
                               /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                               FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                               GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                               YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
               CDS             2875..2892
                               /codon_start=1
                               /product="6xHis affinity tag"
                               /note="6xHis"
                               /translation="HHHHHH"
               CDS             2899..2916
                               /codon_start=1
                               /product="recognition site for phosphorylation by protein 
                               kinase A"
                               /note="PKA site"
                               /translation="RRASVA"
               CDS             2926..2943
                               /codon_start=1
                               /product="thrombin recognition and cleavage site"
                               /note="thrombin site"
                               /translation="LVPRGS"
               misc_feature    2958..3025
                               /note="MCS"
                               /note="multiple cloning site"
               misc_recomb     3045..4503
                               /note="baculovirus recombination region (ORF1629)"
                               /note="contains part of ORF1629"
               CDS             complement(3162..4503)
                               /codon_start=2
                               /product="baculovirus capsid-associated protein"
                               /note="ORF1629"
                               /note="required for viral replication"
                               /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                               LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                               PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                               PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                               APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                               EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                               PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                               IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
               rep_origin      5876..6331
                               /direction=RIGHT
                               /note="f1 ori"
                               /note="f1 bacteriophage origin of replication; arrow 
                               indicates direction of (+) strand synthesis"
               promoter        6613..6717
                               /gene="bla"
                               /note="AmpR promoter"
               CDS             6718..7578
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
               rep_origin      7749..8337
                               /direction=RIGHT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
          ORIGIN
                  1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
                 61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
                121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
                181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
                241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
                301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
                361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
                421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
                481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
                541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
                601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
                661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
                721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
                781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
                841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
                901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
                961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
               1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
               1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
               1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
               1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
               1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
               1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
               1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
               1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
               1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
               1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
               1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
               1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
               1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
               1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
               1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
               1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
               1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
               2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
               2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
               2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
               2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
               2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
               2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
               2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
               2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
               2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
               2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
               2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
               2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
               2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
               2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
               2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
               2941 agcccaggac tcgatggcat atgctcgagg aattcaggcc tccatgggag ctcgcggccg
               3001 cctgcagggt acccccggga gatctgtacc gactctgctg aagaggagga aattctcctt
               3061 gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct gttcactggt
               3121 ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct agattctgtg
               3181 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
               3241 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
               3301 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
               3361 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
               3421 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
               3481 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
               3541 tacaattgac tcgacgtaaa cacgttaaat aaagcttgga catatttaac atcgggcgtg
               3601 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
               3661 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
               3721 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
               3781 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
               3841 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
               3901 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
               3961 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
               4021 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
               4081 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
               4141 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
               4201 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
               4261 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
               4321 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
               4381 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
               4441 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
               4501 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
               4561 agacacttcg ctgtcgtcga cgtacatgta tgctttgttg tcaaaaacgt cgttggcaag
               4621 ctttaaaata tttaaaagaa catctctgtt cagcaccact gtgttgtcgt aaatgttgtt
               4681 tttgataatt tgcgcttccg cagtatcgac acgttcaaaa aattgatgcg catcaatttt
               4741 gttgttccta ttattgaata aataagattg tacagattca tatctacgat tcgtcatggc
               4801 caccacaaat gctacgctgc aaacgctggt acaattttac gaaaactgca aaaacgtcaa
               4861 aactcggtat aaaataatca acgggcgctt tggcaaaata tctattttat cgcacaagcc
               4921 cactagcaaa ttgtatttgc agaaaacaat ttcggcgcac aattttaacg ctgacgaaat
               4981 aaaagttcac cagttaatga gcgaccaccc aaattttata aaaatctatt ttaatcacgg
               5041 ttccatcaac aaccaagtga tcgtgatgga ctacattgac tgtcccgatt tatttgaaac
               5101 actacaaatt aaaggcgagc tttcgtacca acttgttagc aatattatta gacagctgtg
               5161 tgaagcgctc aacgatttgc acaagcacaa tttcatacac aacgacataa aactcgaaaa
               5221 tgtcttatat ttcgaagcac ttgatcgcgt gtatgtttgc gattacggat tgtgcaaaca
               5281 cgaaaactca cttagcgtgc acgacggcac gttggagtat tttagtccgg aaaaaattcg
               5341 acacacaact atgcacgttt cgtttgactg gtacgcggcg tgttaacata caagttgcta
               5401 accggcggcc gacacccatt tgaaaaaagc gaagacgaaa tgttggactt gaatagcatg
               5461 aagcgtcgtc agcaatacaa tgacattggc gttttaaaac acgttcgtaa cgttaacgct
               5521 cgtgactttg tgtactgcct aacaagatac aacatagatt gtagactcac aaattacaaa
               5581 caaattataa aacatgagtt tttgtcgtaa aaatgccact tgttttacga gtagaattag
               5641 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
               5701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
               5761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
               5821 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
               5881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
               5941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
               6001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
               6061 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
               6121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
               6181 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
               6241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
               6301 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
               6361 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
               6421 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
               6481 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
               6541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
               6601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
               6661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
               6721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
               6781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
               6841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
               6901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
               6961 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
               7021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
               7081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
               7141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
               7201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
               7261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
               7321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
               7381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
               7441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
               7501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
               7561 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
               7621 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
               7681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
               7741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
               7801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
               7861 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
               7921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
               7981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
               8041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
               8101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
               8161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
               8221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
               8281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
               8341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
               8401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
               8461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
               8521 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
               8581 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
               8641 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
               8701 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga att
          //
          
          
          

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