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      1. <pre id="q7rs8"></pre>
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        <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

          pYM39-EYFP

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          pYM39EYFP載體基本信息

          出品公司: --
          載體名稱: pYM39-EYFP
          質粒類型: 酵母系列,畢赤酵母表達載體
          終止子: ADH1
          復制子: ori
          啟動子: TEF
          載體大小: 4946bp
          5' 測序引物及序列: T7:TAATACGACTCACTATAGGG
          3' 測序引物及序列: --
          載體標簽: EYFP
          載體抗性: 氨芐青霉素
          篩選標記: --
          克隆菌株: DH5α 等
          宿主細胞(系): 酵母細胞
          備注: --
          產品目錄號: --
          穩定性: --
          組成型/誘導型: --
          病毒/非病毒: --

          pYM39EYFP載體質粒圖譜和多克隆位點信息

          pYM39-EYFP載體圖譜

          pYM39EYFP載體簡介

          pYM39EYFP載體序列

          LOCUS       pYM39-EYFP               4946 bp ds-DNA     circular SYN 19-SEP-2016
          DEFINITION  synthetic circular DNA
          ACCESSION   .
          VERSION     .
          KEYWORDS    Untitled 10
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 4946)
            AUTHORS   .
            TITLE     Direct Submission
            JOURNAL   Exported Monday, September 19, 2016 from SnapGene Viewer 3.2.1
                      http://www.ningxiaxinwen.com/
          FEATURES             Location/Qualifiers
               source          1..4946
                               /organism="synthetic DNA construct"
                               /mol_type="other DNA"
               CDS             79..795
                               /codon_start=1
                               /product="enhanced YFP"
                               /note="EYFP"
                               /note="mammalian codon-optimized"
                               /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                               FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                               NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                               NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                               FVTAAGITLGMDELYK"
               CDS             802..828
                               /codon_start=1
                               /product="HA (human influenza hemagglutinin) epitope tag"
                               /note="HA"
                               /translation="YPYDVPDYA"
               terminator      861..1048
                               /gene="S. cerevisiae ADH1"
                               /note="ADH1 terminator"
                               /note="transcription terminator for the S. cerevisiae 
                               alcohol dehydrogenase 1 (ADH1) gene"
               gene            1123..2479
                               /note="kanMX"
                               /note="yeast selectable marker conferring kanamycin 
                               resistance (Wach et al., 1994)"
               promoter        1123..1466
                               /note="TEF promoter"
                               /note="Ashbya gossypii TEF promoter"
               CDS             1467..2276
                               /codon_start=1
                               /gene="aph(3')-Ia"
                               /product="aminoglycoside phosphotransferase"
                               /note="KanR"
                               /note="confers resistance to kanamycin"
                               /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                               DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                               FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                               FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                               RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
               terminator      2282..2479
                               /note="TEF terminator"
                               /note="Ashbya gossypii TEF terminator"
               promoter        complement(2584..2602)
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               rep_origin      complement(2860..3448)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(3619..4479)
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
               promoter        complement(4480..4584)
                               /gene="bla"
                               /note="AmpR promoter"
               promoter        4930..2
                               /note="SP6 promoter"
                               /note="promoter for bacteriophage SP6 RNA polymerase"
          ORIGIN
                  1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct
                 61 ggtgctggtg ctggagcaat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
                121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
                181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
                241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tgcagtgctt cgcccgctac
                301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
                361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
                421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
                481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
                541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
                601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
                661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
                721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
                781 gagctgtaca agtaaggatc ctatccatat gacgttccag attacgctgc tcagtgctag
                841 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
                901 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
                961 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
               1021 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga
               1081 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
               1141 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
               1201 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
               1261 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc
               1321 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
               1381 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
               1441 cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc
               1501 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
               1561 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
               1621 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
               1681 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
               1741 catggttact caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc
               1801 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
               1861 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
               1921 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
               1981 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
               2041 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
               2101 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
               2161 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
               2221 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
               2281 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
               2341 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
               2401 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
               2461 aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag tttaaacgag
               2521 ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt
               2581 ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg
               2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
               2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
               2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
               2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
               2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
               2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
               3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
               3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
               3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
               3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
               3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
               3301 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
               3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
               3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
               3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
               3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
               3601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
               3661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
               3721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
               3781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
               3841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
               3901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
               3961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
               4021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
               4081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
               4141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
               4201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
               4261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
               4321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
               4381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
               4441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
               4501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
               4561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
               4621 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
               4681 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
               4741 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
               4801 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
               4861 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
               4921 acacatacga tttaggtgac actata
          //
          
          
          

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