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          pYES2-NTB

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Invitrogen
          載體名稱: pYES2-NTB, pYES2/NT B
          質粒類型: 釀酒酵母蛋白表達載體
          表達水平: 高拷貝
          誘導方法: 半乳糖
          啟動子: GAL1
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 6039 bp
          5' 測序引物及序列: T7: TAATACGACTCACTATAGGG
          3' 測序引物及序列: CYC1 Terminator: GTGACATAACTAATTACATGATG
          載體標簽: N-His, N-Xpress, C-His, C-V5
          載體抗性: 氨芐
          篩選標記: URA3
          備注: 利用半乳糖誘導蛋白在釀酒酵母中表達。
          產品目錄號: V8252-20
          穩定性: 穩定 Stable
          組成型/誘導型: 誘導型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pYES2-NTB載體圖譜

          pYES2-NTB多克隆位點

          載體簡介

          pYES2 NT B的是一個6.0 kb的載體,設計用來在釀酒酵母(Saccharomyces cerevisiae)中誘導表達重組蛋白。

          該載體包含以下元素:

          1. 酵母GAL1啟動子,能夠在釀酒酵母中被半乳糖高水平的誘導蛋白表達目的蛋白,同時能夠被葡萄糖抑制表達

          2.多克隆位點可以使用的很多限制酶切位點,便于基因插入。

          3.CYC1終止子能夠有效終止mRNA的轉錄。

          4.能夠利用URA3基因篩選帶有ura3基因型的酵母宿主菌株轉化子。

          5.氨芐抗性基因能夠方便在大腸桿菌中的進行載體篩選。

          6. 載體本身帶有N端His和Xpress標簽,同時載體C端也帶有V5抗原表位和His純化標簽。

          8. 在酵母中,利用載體上的2μ復制子維持質粒的高拷貝。

          載體序列

          LOCUS       pYES2/NT\B	6039 bp 	DNA     circular	SYN
          DEFINITION  pYES2/NT B
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     VNTAUTHORNAME|biofeng.com|
          FEATURES             Location/Qualifiers
               source          1..6039
                               /organism="pYES2/NT B"
                               /mol_type="other DNA"
               promoter        1..451
                               /label="GAL1_promoter"
               misc_feature    414..437
                               /label="GAL1_primer"
               promoter        475..493
                               /label="T7_promoter"
               misc_feature    542..560
                               /label="Xpress_fwd_primer"
               misc_feature    543..575
                               /label="T7_leader"
               misc_feature    579..602
                               /label="Xpress_EK"
               terminator      792..1037
                               /label="CYC1_terminator"
               misc_feature    complement(801..819)
                               /label="CYC1_primer"
               misc_feature    complement(801..819)
                               /label="pYESTrp_rev_primer"
               rep_origin      complement(1265..1884)
                               /label="pBR322_origin"
               CDS             complement(2039..2899)
                               /label="ORF frame 3"
               CDS             complement(2995..3798)
                               /label="ORF frame 1"
               gene            complement(2998..3798)
                               /label="URA3"
                               /gene="URA3"
               CDS             3287..3763
                               /label="ORF frame 2"
               promoter        complement(3800..4025)
                               /label="URA3_promoter"
               rep_origin      4028..5499
                               /label="2micron_origin"
          ORIGIN
              1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
             61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
            121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
            181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
            241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
            301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
            361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
            421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
            481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
            541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
            601 AGGTACCTAA GGATCCAGTG TGGTGGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT
            661 CGAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
            721 TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CCTAGAGGGC
            781 CGCATCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC CTCCCCCCAC ATCCGCTCTA
            841 ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT
            901 ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC
            961 GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG
           1021 GCTTTAATTT GCAAGCTGCG GCCCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
           1081 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
           1141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
           1201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGCCCAGG AACCGTAAAA
           1261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
           1321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
           1381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
           1441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
           1501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
           1561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
           1621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
           1681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
           1741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
           1801 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
           1861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
           1921 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
           1981 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
           2041 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
           2101 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GCGCTTACCA TCTGGCCCCA
           2161 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
           2221 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
           2281 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
           2341 TTGTTGGCAT TGCTACAGGC ATCGTGGTGT CACTCTCGTC GTTTGGTATG GCTTCATTCA
           2401 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
           2461 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
           2521 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
           2581 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
           2641 CTTGCCCGGC GTCAATACGG GATAATAGTG TATCACATAG CAGAACTTTA AAAGTGCTCA
           2701 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
           2761 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
           2821 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
           2881 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATGGGT AATAACTGAT ATAATTAAAT
           2941 TGAAGCTCTA ATTTGTGAGT TTAGTATACA TGCATTTACT TATAATACAG TTTTTTAGTT
           3001 TTGCTGGCCG CATCTTCTCA AATATGCTTC CCAGCCTGCT TTTCTGTAAC GTTCACCCTC
           3061 TACCTTAGCA TCCCTTCCCT TTGCAAATAG TCCTCTTCCA ACAATAATAA TGTCAGATCC
           3121 TGTAGAGACC ACATCATCCA CGGTTCTATA CTGTTGACCC AATGCGTCTC CCTTGTCATC
           3181 TAAACCCACA CCGGGTGTCA TAATCAACCA ATCGTAACCT TCATCTCTTC CACCCATGTC
           3241 TCTTTGAGCA ATAAAGCCGA TAACAAAATC TTTGTCGCTC TTCGCAATGT CAACAGTACC
           3301 CTTAGTATAT TCTCCAGTAG ATAGGGAGCC CTTGCATGAC AATTCTGCTA ACATCAAAAG
           3361 GCCTCTAGGT TCCTTTGTTA CTTCTTCTGC CGCCTGCTTC AAACCGCTAA CAATACCTGG
           3421 GCCCACCACA CCGTGTGCAT TCGTAATGTC TGCCCATTCT GCTATTCTGT ATACACCCGC
           3481 AGAGTACTGC AATTTGACTG TATTACCAAT GTCAGCAAAT TTTCTGTCTT CGAAGAGTAA
           3541 AAAATTGTAC TTGGCGGATA ATGCCTTTAG CGGCTTAACT GTGCCCTCCA TGGAAAAATC
           3601 AGTCAAGATA TCCACATGTG TTTTTAGTAA ACAAATTTTG GGACCTAATG CTTCAACTAA
           3661 CTCCAGTAAT TCCTTGGTGG TACGAACATC CAATGAAGCA CACAAGTTTG TTTGCTTTTC
           3721 GTGCATGATA TTAAATAGCT TGGCAGCAAC AGGACTAGGA TGAGTAGCAG CACGTTCCTT
           3781 ATATGTAGCT TTCGACATGA TTTATCTTCG TTTCCTGCAG GTTTTTGTTC TGTGCAGTTG
           3841 GGTTAAGAAT ACTGGGCAAT TTCATGTTTC TTCAACACTA CATATGCGTA TATATACCAA
           3901 TCTAAGTCTG TGCTCCTTCC TTCGTTCTTC CTTCTGTTCG GAGATTACCG AATCAAAAAA
           3961 ATTTCAAAGA AACCGAAATC AAAAAAAAGA ATAAAAAAAA AATGATGAAT TGAATTGAAA
           4021 AGCTAGCTTA TCGATGATAA GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA
           4081 TACTAGATAC TCCGTCTACT GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG
           4141 GCCTTACCAC TCTTTTGTTA CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA
           4201 TTCTATCTTC GCGATGTAGT AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG
           4261 GCACTTCTAC AATGGCTGCC ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT
           4321 ATTCGAATAC GCTTTGAGGA GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC
           4381 GATCGTACTT GTTACCCATC ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA
           4441 ACAGATAGTA TATTTGAACC TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT
           4501 GTATGTATTT CGGTTCCTGG AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC
           4561 GCATCCCCGG TTCATTTTCT GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC
           4621 GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA
           4681 ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC
           4741 CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA
           4801 TTTTTCAAAC AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC
           4861 TATTTTACCA ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC
           4921 GCTATTTTTC TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG
           4981 CAGTCTCTTG ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG
           5041 TGTCTATTTT CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA
           5101 GCGAAGCTGC GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT
           5161 GTGGATTGCG CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG
           5221 AAAATTATGA ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA
           5281 TTTTCGTATT GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA
           5341 GAGTAATACT AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG
           5401 AGCGAAAGGT GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA
           5461 TACTTTTGAG CAATGTTTGT GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT
           5521 AATCAGAAAA GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT
           5581 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG
           5641 CCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT
           5701 GTTCCAGTTT CCAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA
           5761 AAAAGGGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
           5821 GGGTCGAGGT GCCGTAAAGC AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT
           5881 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG
           5941 GCTAGGGCGG TGGGAAGTGT AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT
           6001 AATGGGGCGC TACAGGGCGC GTGGGGATGA TCCACTAGT
          //
          

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