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          pYC66

          價格:1200元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品人: Irene Castano Navarro(2015)
          載體名稱: pYC66
          質粒類型: 釀酒酵母載體;復制型載體;抗性篩選載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子:
          載體大小: 5085 bp
          5' 測序引物及序列: M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’
          3' 測序引物及序列: M13 rev primer 5’ GGAAACAGCTATGACCATG 3’
          載體標簽: 3XMyc tag (C-ter)
          載體抗性: 氨芐青霉素
          篩選標記: Nourseothricin
          克隆菌株: DH10B感受態細胞 ,30°C
          宿主細胞(系): 出芽酵母(釀酒酵母)、白色念珠菌
          備注: --
          產品目錄號: #63929
          穩定性: 瞬表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pYC66載體圖譜



          pYC66多克隆位點

          載體簡介

          載體序列

          LOCUS       pYC66	5085 bp 	DNA	SYN
          DEFINITION  pYC66
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..5085
                               /organism="pYC66"
                               /mol_type="other DNA"
               CDS             761..1333
                               /label="ORF frame 2"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               terminator      1347..1586
                               /label="CYC1_terminator"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               misc_feature    complement(1356..1374)
                               /label="CYC1_primer"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               misc_feature    complement(1356..1374)
                               /label="pYESTrp_rev_primer"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               misc_feature    1608..1624
                               /label="pBluescriptKS_primer"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               promoter        complement(2287..2306)
                               /label="T3_promoter"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               promoter        complement(2323..2341)
                               /label="M13_reverse_primer"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               misc_feature    complement(2340..2362)
                               /label="M13_pUC_rev_primer"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               promoter        complement(2376..2405)
                               /label="lac_promoter"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               gene            complement(3488..4348)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               CDS             complement(3488..4348)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(4390..4418)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcaCctcccg gagacggtca
             61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
            121 ttggcgggtg tcggggctgg cttaactatg cgGCATCAGA GCAGATTGTA CTGAGAGTGC
            181 ACCATAccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
            241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
            301 agcacagact tagattggta tatatacgCA TACcataaag cacAGCTCca tagcttcaaa
            361 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca
            421 cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt
            481 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc
            541 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga
            601 tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc
            661 tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag
            721 aaagaaagca tagcaatcta atctaagttt tggatctaaa ATGGGCACCA CTCTTGACGA
            781 CACGGCTTAC CGATACCGCA CCAGTGTCCC GGGGGACGCC GAGGCCATCG AGGCACTGGA
            841 TGGGTCCTTC ACCACCGACA CCGTCTTCCG CGTCACCGCC ACCGGGGACG GCTTCACCCT
            901 GCGGGAGGTG CCGGTGGACC CGCCCCTGAC CAAGGTGTTC CCCGACGACG AATCGGACGA
            961 CGAATCGGAC GACGGGGAGG ACGGCGACCC TGACTCCCGG ACGTTCGTCG CGTACGGGGA
           1021 CGACGGCGAC CTGGCGGGCT TCGTGGTCGT CTCGTACTCC GGCTGGAACC GCCGGCTGAC
           1081 CGTCGAGGAC ATCGAGGTCG CCCCGGAGCA CCGGGGGCAC GGGGTCGGGC GCGCGTTGAT
           1141 GGGGCTCGCG ACGGAGTTCG CCCGCGAGCT GGGCGCCGGG CACCTCTGGC TGGAGGTCAC
           1201 CAACGTCAAC GCACCGGCGA TCCACGCGTA CCGGCGGATG GGGTTCACCC TCTGCGGCCT
           1261 GGACACCGCC CTGTACGACG GCACCGCCTC GGACGGCGAG CAGGCGCTCT ACATGAGCAT
           1321 GCCCTGCCCC TAAgtcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
           1381 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
           1441 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
           1501 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
           1561 ttgggacgct cgaaggcttt aatttgcggc cGGTACcggg ccccccctcg aggtcgacgg
           1621 tatcgataag cttgatatcg aattcctgca gcccggggga tcctagggTT AATTAACGGT
           1681 GAACAAAAGC TAATCTCCGA GGAAGACTTG AACGGTGAAC AAAAATTAAT CTCAGAAGAA
           1741 GACTTGAACG GACTCGACGG TGAACAAAAG TTGATTTCTG AAGAAGATTT GAACGGTGAA
           1801 CAAAAGCTAA TCTCCGAGGA AGACTTGAAC GGTGAACAAA AATTAATCTC AGAAGAAGAC
           1861 TTGAACGGAC TCGACGGTGA ACAAAAGTTG ATTTCTGAAG AAGATTTGAA CGGTGAACAA
           1921 AAGCTAATCT CCGAGGAAGA CTTGAACGGT GAACAAAAAT TAATCTCAGA AGAAGACTTG
           1981 AACGGACTCG ACGGTGAACA AAAGTTGATT TCTGAAGAAG ATTTGAACGG TGAACAAAAG
           2041 CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA TCTCAGAAGA AGACTTGAAC
           2101 GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT TGAACGGTGA ACAAAAGCTA
           2161 ATCTCCGAGG AAGACTTGAA CGGTGAACAA AAATTAATCA ATCACtagct agtgaattcg
           2221 cgccagatcc GAAGTTCCTA TACTTTctag agcggccgcc accgCGGTGG AGCTCCAGCT
           2281 TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC
           2341 Ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat aCgagccgga agcataaagt
           2401 gtaaagcctg gggtgcctaa tgagtgagCt aactcacatt aattgcgttg cgctcactgc
           2461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
           2521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
           2581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
           2641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
           2701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
           2761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
           2821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
           2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
           2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
           3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
           3061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
           3121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
           3181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
           3241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
           3301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
           3361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
           3421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
           3481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
           3541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
           3601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
           3661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
           3721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
           3781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
           3841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
           3901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
           3961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
           4021 gcttttctgt gactggtgAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
           4081 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
           4141 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
           4201 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
           4261 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
           4321 GGGCGACACG GAAATGTTGA ATACTCATac tcttcctttt tcaatattat tgaagcattt
           4381 atcagggtta ttgtctcatg agcggataca taTTTGAATG TGGGGATCTC TAATGATGCA
           4441 ATTTTTGTTT TGTACACTAA GATTCTATTT CGGAACTCTT ACCCATGTCA TTATTTactt
           4501 tattttcatt attgTAATTT TTTAAGCTTT TATTATGAAT TATTTAAATA CAATTCTTTT
           4561 TGATCATGTG ACAGACATCA CTGAAAGATG TGCTCTGCGT TATTAGTCAG ATTGAAATAT
           4621 ACATTTTCAT GTAGATATGA TGTTCTTACT TTAAATGTTG TTCCATTTGC TAGCTTAAAT
           4681 AGACGATATT ATAAATTTAA GTATTCACTA AAAATCTGAC CCATTAATGT TAGAATTAGT
           4741 ATTATTTTTT GTGATATTAC ATTTTTATAT TTTGGATTTC AACCAATGCT CTGCGATATG
           4801 ATTCAACAGA ATAATATGTA CAAGCTACTT TAATTCATAT ATACTAATAG ATTCACAGAT
           4861 CCAGTACGAC TGCAAGAATT TACGTTTTGT TTGAAATGGT AGTAGCTACA CTAAATTAAC
           4921 AATGATCGAT TAATCCACAT CCACAGTGTG TAATCATCCT AATGAAAACA AGAATAACTA
           4981 CAAAAATGAA TATAAACTTT TTGCATGTTA GTCTAACCTG CTAAGAAACC ATTATTATCA
           5041 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTC
          //
          
          

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