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          pYC46

          價格:1200元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品人: Irene Castano Navarro(2015)
          載體名稱: pYC46
          質粒類型: 釀酒酵母表達載體;整合型載體;FLP/FRT系統載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: --
          載體大小: 6017 bp
          5' 測序引物及序列: M13 primer  5’TGTAAAACGACGGCCAGT 3’
          3' 測序引物及序列: --
          載體標簽: 3XFLAG tag (C ter)
          載體抗性: 氨芐青霉素
          篩選標記: URA3,Nourseothricin
          克隆菌株: DH10B感受態細胞 ,30°C
          宿主細胞(系): 出芽酵母(釀酒酵母)、白色念珠菌
          備注: --
          產品目錄號: 63904
          穩定性: 瞬表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pYC46載體圖譜



          pYC46多克隆位點

          載體簡介

          釀酒酵母整合型載體還有: 

          pYC44
          pYC56   紅色熒光標簽
          pYC54   黃色熒光標簽
          pYC52   青色熒光標簽
          pYC50   13XMyc標簽
          pYC48   HA標簽
          pYC46   3XFlag標簽



          載體序列

          LOCUS       pYC46	6017 bp 	DNA	SYN
          DEFINITION  pYC46
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..6017
                               /organism="pYC46"
                               /mol_type="other DNA"
               misc_feature    complement(29..51)
                               /label="pGEX_3_primer"
               promoter        196..409
                               /label="URA3_promoter"
               gene            411..1211
                               /label="URA3"
                               /gene="URA3"
               CDS             411..1214
                               /label="ORF frame 3"
                               /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                               LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                               LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                               TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                               TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
               CDS             complement(446..922)
                               /label="ORF frame 2"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               misc_feature    complement(1814..1962)
                               /label="lacZ_a"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               misc_feature    1928..1950
                               /label="M13_pUC_fwd_primer"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               promoter        1943..1959
                               /label="M13_forward20_primer"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               promoter        1966..1984
                               /label="T7_promoter"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               misc_feature    2017..2033
                               /label="pBluescriptSK_primer"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               misc_feature    2052..2063
                               /label="EK"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               misc_feature    2112..2126
                               /label="EK"
                               /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                               GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                               EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
               CDS             complement(2823..3326)
                               /label="ORF frame 1"
                               /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
                               NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
                               INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
                               PVVYVRM*"
               CDS             2862..3434
                               /label="ORF frame 3"
                               /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               promoter        complement(3756..3775)
                               /label="T3_promoter"
                               /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               promoter        complement(3792..3810)
                               /label="M13_reverse_primer"
                               /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               misc_feature    complement(3809..3831)
                               /label="M13_pUC_rev_primer"
                               /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               rep_origin      complement(4183..4802)
                               /label="pBR322_origin"
                               /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               gene            complement(4957..5817)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                               FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                               NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                               GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
               CDS             complement(4957..5817)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(5859..5887)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
             61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
            121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
            181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
            241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
            301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
            361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
            421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
            481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
            541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
            601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
            661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
            721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
            781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
            841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
            901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
            961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
           1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
           1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
           1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
           1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
           1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
           1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
           1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
           1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
           1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
           1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
           1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
           1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
           1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
           1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
           1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
           1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
           1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctaGGAG
           2041 CAGACTACAA AGATGACGAT GACAAaGgaG CCGATTATAA GGACGATGAC GATAAAGGGG
           2101 CTGACTACAA AGATGACGAT GACAAGtgat aagatccGAA GTTCCTATAC TTTCTAGAGA
           2161 ATAGGAACTT Cgtgcgcttt tgaaccacgt aaagtgctgt aaaCcagctc tattcaatga
           2221 ggtaaatgat aaagtttata agagaccaaa aaattcaaaa aaaaaattga aaaattatat
           2281 tggtatttat atgaaacaat agttcagttc aaagcgctag cttttctcat ctcaactaac
           2341 cggtattagg tgggtattta tttaatatgg gaacaagaaa tgtgacaTta atgttcatgt
           2401 ttttattttc attatttatt ctatatggtt tgggacttga tgttatgtct taattcatag
           2461 aGATCCgctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
           2521 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
           2581 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
           2641 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
           2701 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
           2761 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
           2821 catcacatcc gaacataaac aaccgGATCg atcctacaat tATGTCTACT ACTTTGGATG
           2881 ATACTGCTTA TAGATACAGA ACTTCTGTTC CAGGTGATGC TGAAGCTATT GAAGCTTTGG
           2941 ATGGTTCTTT CACTACCGAT ACTGTTTTTA GAGTTACTGC TACTGGTGAT GGTTTCACTT
           3001 TGAGAGAAGT TCCAGTTGAT CCACCATTGA CTAAGGTTTT CCCAGATGAT GAATCCGATG
           3061 ATGAATCCGA TGCTGGTGAA GATGGTGATC CAGATTCTAG AACTTTCGTT GCTTATGGTG
           3121 ATGATGGTGA TTTGGCTGGT TTCGTTGTTG TTTCTTATTC TGGTTGGAAC AGAAGATTGA
           3181 CTGTTGAAGA TATTGAAGTT GCTCCAGAAC ATAGAGGTCA TGGTGTTGGT AGAGCTTTGA
           3241 TGGGTTTGGC TACTGAATTC GCCAGAGAAA GAGGTGCTGG TCATTTGTGG TTGGAAGTTA
           3301 CCAATGTTAA TGCTCCAGCT ATTCATGCTT ATAGAAGAAT GGGTTTCACT TTGTGTGGTT
           3361 TGGATACTGC TTTATACGAT GGTACTGCTT CCGATGGTGA ACAAGCTTTG TATATGTCTA
           3421 TGCCATGTCC ATAAtaacTC GACcagtact gacaataaaa agattcttgt tttcaagaac
           3481 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
           3541 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
           3601 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
           3661 taacgccgcc atccagtgTC GAtcgacGAA GTTCCTATAC TTTCTAGAGA ATAGGAACTT
           3721 CGTCctcgag ggggggcccg gtaccagctt ttgttccctt tagtgagggt taattccgag
           3781 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
           3841 acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta
           3901 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
           3961 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
           4021 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
           4081 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
           4141 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gcCGCGTTGC TGGCGTTTTT
           4201 CCATAGGCTC GGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
           4261 AAACCCGACA GGACTATAAA GATACCAGGC GTTCCCCCCT GGAAGCTCCC TCGTGCGCTC
           4321 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
           4381 GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
           4441 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
           4501 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
           4561 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
           4621 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
           4681 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
           4741 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
           4801 CTtttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
           4861 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
           4921 aatctaaagt atatatgagt aaacttggtc tgacagTTAC CAATGCTTAA TCAGTGAGGC
           4981 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTGC CCGTCGTGTA
           5041 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
           5101 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
           5161 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
           5221 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
           5281 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
           5341 GCGAGTTACA TGATCCCCCA TGTTGTGAAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
           5401 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
           5461 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
           5521 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
           5581 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
           5641 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
           5701 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
           5761 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATact
           5821 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
           5881 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
           5941 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
           6001 cacgaggccc tttcgtc
          //
          
          

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