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          pGADT7

          價格:1300元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: clotech
          載體名稱: pGADT7
          質粒類型: 釀酒酵母表達載體
          高拷貝/低拷貝: --
          啟動子: ADH1
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 7988bp
          5' 測序引物及序列: T7: TAATACGACTCACTATAGGG
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: Ampicillin
          篩選標記: --
          備注: --
          產品目錄號: 630442
          穩定性: --
          組成型: --
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pGADT7載體圖譜



          pGADT7多克隆位點

          pGADT7載體特征

          載體簡介

          pGADT7 AD is a yeast expression vector that is designed to express a protein of interest
          fused to a GAL4 activation domain (AD; amino acids 768–881). Transcription of the GAL4 AD
          fusion is driven by the constitutively active ADH1 promoter (PADH1), and is terminated at the
          ADH1 transcription termination signal (TADH1). The GAL4 AD fusion contains an N-terminal
          SV40 nuclear localization signal (SV40 NLS; 1) that targets the protein to the yeast nucleus,
          and a hemagglutinin epitope tag (HA Tag), located between the GAL4 AD and the protein
          of interest, that allows the protein to be easily detected with HA-tag antibodies.
          The T7 promoter (PT7), located just upstream of the HA tag sequence, allows in vitro transcription
          and translation of the HA-tagged protein of interest (without the GAL4 AD and
          the SV40 NLS). pGADT7 AD replicates autonomously in both E. coli and S. cerevisiae from
          the pUC and 2 ori, respectively. The vector also contains an ampicillin resistance gene
          (Ampr) for selection in E. coli and a LEU2 nutritional marker for selection in yeast.
          
          

          載體序列

          LOCUS       pGAD T7    7988 bp    DNA    SYN    23-Nov-2013
          DEFINITION  pGAD T7
          ACCESSION    
          KEYWORDS    
          SOURCE    
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..7988
                               /organism="pGAD T7"
                               /mol_type="other DNA"
               promoter        1079..1476
                               /label="yADH1_promoter"
               misc_feature    1501..1557
                               /label="SV40_NLS"
               misc_feature    1492..1899
                               /label="Gal4_AD"
               misc_feature    1561..1899
                               /label="Gal4_AD"
               misc_feature    1825..1842
                               /label="Gal4_AD_primer"
               promoter        1905..1923
                               /label="T7_promoter"
               misc_feature    complement(2295..2318)
                               /label="pJG4_5_rev_primer"
               terminator      2279..2605
                               /label="yADH2_terminator"
               misc_feature    complement(2471..2495)
                               /label="pHybLex_rev_primer"
               terminator      2416..2595
                               /label="yADH1_terminator"
               CDS             complement(2720..3814)
                               /label="LEU2(variant)"
                               /gene="LEU2(variant)"
                               /note="ORF frame 2"
                               /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
                               LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
                               YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
                               QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
                               VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
                               KNTAFGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
                               AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
               gene            complement(2720..3814)
                               /label="LEU2(variant)"
                               /gene="LEU2(variant)"
               misc_feature    complement(4258..4276)
                               /label="M13_reverse_primer"
               misc_feature    complement(4275..4297)
                               /label="M13_pUC_rev_primer"
               promoter        complement(4311..4340)
                               /label="lac_promoter"
               misc_feature    complement(4431..4464)
                               /label="loxP"
               misc_feature    complement(4542..4575)
                               /label="loxP"
               rep_origin      complement(4800..5419)
                               /label="pBR322_origin"
               CDS             complement(5574..6434)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /note="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               gene            complement(5574..6434)
                               /label="Ampicillin"
                               /gene="Ampicillin"
               promoter        complement(6476..6504)
                               /label="AmpR_promoter"
               misc_feature    complement(6663..6685)
                               /label="pGEX_3_primer"
               rep_origin      6821..7985
                               /label="2micron2_origin"
          ORIGIN
              1 TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC 
             61 AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT 
            121 TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA 
            181 TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC 
            241 TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG 
            301 AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG 
            361 GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA 
            421 GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT 
            481 GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG 
            541 GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT 
            601 ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC 
            661 ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC 
            721 CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT 
            781 TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA 
            841 TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT 
            901 AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC 
            961 ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA 
           1021 TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA 
           1081 ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT 
           1141 GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA 
           1201 GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC 
           1261 TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT 
           1321 GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA 
           1381 TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC 
           1441 ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA 
           1501 GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC 
           1561 AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT 
           1621 AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT 
           1681 GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT 
           1741 GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG 
           1801 TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA 
           1861 TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT 
           1921 AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG 
           1981 GAGGCCAGTG AATTCCACCC GGGTGGGCAT CGATACGGGA TCCATCGAGC TCGAGCTGCA 
           2041 GATGAATCGT AGATACTGAA AAACCCCGCA AGTTCACTTC AACTGTGCAT CGTGCACCAT 
           2101 CTCAATTTCT TTCATTTATA CATCGTTTTG CCTTCTTTTA TGTAACTATA CTCCTCTAAG 
           2161 TTTCAATCTT GGCCATGTAA CCTCTGATCT ATAGAATTTT TTAAATGACT AGAATTAATG 
           2221 CCCATCTTTT TTTTGGACCT AAATTCTTCA TGAAAATATA TTACGAGGGC TTATTCAGAA 
           2281 GCTTTGGACT TCTTCGCCAG AGGTTTGGTC AAGTCTCCAA TCAAGGTTGT CGGCTTGTCT 
           2341 ACCTTGCCAG AAATTTACGA AAAGATGGAA AAGGGTCAAA TCGTTGGTAG ATACGTTGTT 
           2401 GACACTTCTA AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA 
           2461 AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA 
           2521 TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGCA TGAGGTCGCT 
           2581 CTTATTGACC ACACCTCTAC CGGCCGGTCG AAATTCCCCT ACCCTATGAA CATATTCCAT 
           2641 TTTGTAATTT CGTGTCGTTT CTATTATGAA TTTCATTTAT AAAGTTTATG TACAAATATC 
           2701 ATAAAAAAAG AGAATCTTTT TAAGCAAGGA TTTTCTTAAC TTCTTCGGCG ACAGCATCAC 
           2761 CGACTTCGGT GGTACTGTTG GAACCACCTA AATCACCAGT TCTGATACCT GCATCCAAAA 
           2821 CCTTTTTAAC TGCATCTTCA ATGGCCTTAC CTTCTTCAGG CAAGTTCAAT GACAATTTCA 
           2881 ACATCATTGC AGCAGACAAG ATAGTGGCGA TAGGGTTGAC CTTATTCTTT GGCAAATCTG 
           2941 GAGCAGAACC GTGGCATGGT TCGTACAAAC CAAATGCGGT GTTCTTGTCT GGCAAAGAGG 
           3001 CCAAGGACGC AGATGGCAAC AAACCCAAGG AACCTGGGAT AACGGAGGCT TCATCGGAGA 
           3061 TGATATCACC AAACATGTTG CTGGTGATTA TAATACCATT TAGGTGGGTT GGGTTCTTAA 
           3121 CTAGGATCAT GGCGGCAGAA TCAATCAATT GATGTTGAAC CTTCAATGTA GGAAATTCGT 
           3181 TCTTGATGGT TTCCTCCACA GTTTTTCTCC ATAATCTTGA AGAGGCCAAA ACATTAGCTT 
           3241 TATCCAAGGA CCAAATAGGC AATGGTGGCT CATGTTGTAG GGCCATGAAA GCGGCCATTC 
           3301 TTGTGATTCT TTGCACTTCT GGAACGGTGT ATTGTTCACT ATCCCAAGCG ACACCATCAC 
           3361 CATCGTCTTC CTTTCTCTTA CCAAAGTAAA TACCTCCCAC TAATTCTCTG ACAACAACGA 
           3421 AGTCAGTACC TTTAGCAAAT TGTGGCTTGA TTGGAGATAA GTCTAAAAGA GAGTCGGATG 
           3481 CAAAGTTACA TGGTCTTAAG TTGGCGTACA ATTGAAGTTC TTTACGGATT TTTAGTAAAC 
           3541 CTTGTTCAGG TCTAACACTA CCTGTACCCC ATTTAGGACC ACCCACAGCA CCTAACAAAA 
           3601 CGGCATCAAC CTTCTTGGAG GCTTCCAGCG CCTCATCTGG AAGTGGGACA CCTGTAGCGT 
           3661 CGATAGCAGC ACCACCAATT AAATGATTTT CGAAATCGAA CTTGACATTG GAACGAACAT 
           3721 CAGAAATAGC TTTAAGAACC TTAATGGCTT CGGCTGTGAT TTCTTGACCA ACGTGGTCAC 
           3781 CTGGCAAAAC GACGATCTTC TTAGGGGCAG ACATTAGAAT GGTATATCCT TGAAATATAT 
           3841 ATATATATTG CTGAAATGTA AAAGGTAAGA AAAGTTAGAA AGTAAGACGA TTGCTAACCA 
           3901 CCTATTGGAA AAAACAATAG GTCCTTAAAT AATATTGTCA ACTTCAAGTA TTGTGATGCA 
           3961 AGCATTTAGT CATGAACGCT TCTCTATTCT ATATGAAAAG CCGGTTCCGG CGCTCTCACC 
           4021 TTTCCTTTTT CTCCCAATTT TTCAGTTGAA AAAGGTATAT GCGTCAGGCG ACCTCTGAAA 
           4081 TTAACAAAAA ATTTCCAGTC ATCGAATTTG ATTCTGTGCG ATAGCGCCCC TGTGTGTTCT 
           4141 CGTTATGTTG AGGAAAAAAA TAATGGTTGC TAAGAGATTC GAACTCTTGC ATCTTACGAT 
           4201 ACCTGAGTAT TCCCACAGTT GGGGATCTCG ACTCTAGCTA GAGGATCAAT TCGTAATCAT 
           4261 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
           4321 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
           4381 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG ATAACTTCGT 
           4441 ATAATGTATG CTATACGAAG TTATTAGGTC TGAAGAGGAG TTTACGTCCA GCCAAGCTAG 
           4501 CTTGGCTGCA GGTCGAGCGG CCGCGATCCG GAACCCTTAA TATAACTTCG TATAATGTAT 
           4561 GCTATACGAA GTTATCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 
           4621 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 
           4681 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 
           4741 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 
           4801 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 
           4861 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 
           4921 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 
           4981 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 
           5041 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 
           5101 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 
           5161 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 
           5221 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG 
           5281 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 
           5341 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 
           5401 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 
           5461 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 
           5521 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 
           5581 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 
           5641 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 
           5701 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 
           5761 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 
           5821 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 
           5881 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 
           5941 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 
           6001 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 
           6061 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 
           6121 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 
           6181 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 
           6241 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 
           6301 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 
           6361 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 
           6421 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
           6481 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
           6541 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC 
           6601 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA 
           6661 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG 
           6721 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC 
           6781 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA ACGCATTTAA GCATAAACAC 
           6841 GCACTATGCC GTTCTTCTCA TGTATATATA TATACAGGCA ACACGCAGAT ATAGGTGCGA 
           6901 CGTGAACAGT GAGCTGTATG TGCGCAGCTC GCGTTGCATT TTCGGAAGCG CTCGTTTTCG 
           6961 GAAACGCTTT GAAGTTCCTA TTCCGAAGTT CCTATTCTCT AGCTAGAAAG TATAGGAACT 
           7021 TCAGAGCGCT TTTGAAAACC AAAAGCGCTC TGAAGACGCA CTTTCAAAAA ACCAAAAACG 
           7081 CACCGGACTG TAACGAGCTA CTAAAATATT GCGAATACCG CTTCCACAAA CATTGCTCAA 
           7141 AAGTATCTCT TTGCTATATA TCTCTGTGCT ATATCCCTAT ATAACCTACC CATCCACCTT 
           7201 TCGCTCCTTG AACTTGCATC TAAACTCGAC CTCTACATTT TTTATGTTTA TCTCTAGTAT 
           7261 TACTCTTTAG ACAAAAAAAT TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC 
           7321 GAAAATGTAA ACATTTCCTA TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA 
           7381 ATTTTCTGAC CAATGAAGAA TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA 
           7441 TCCACATCGG TATAGAATAT AATCGGGGAT GCCTTTATCT TGAAAAAATG CACCCGCAGC 
           7501 TTCGCTAGTA ATCAGTAAAC GCGGGAAGTG GAGTCAGGCT TTTTTTATGG AAGAGAAAAT 
           7561 AGACACCAAA GTAGCCTTCT TCTAACCTTA ACGGACCTAC AGTGCAAAAA GTTATCAAGA 
           7621 GACTGCATTA TAGAGCGCAC AAAGGAGAAA AAAAGTAATC TAAGATGCTT TGTTAGAAAA 
           7681 ATAGCGCTCT CGGGATGCAT TTTTGTAGAA CAAAAAAGAA GTATAGATTC TTTGTTGGTA 
           7741 AAATAGCGCT CTCGCGTTGC ATTTCTGTTC TGTAAAAATG CAGCTCAGAT TCTTTGTTTG 
           7801 AAAAATTAGC GCTCTCGCGT TGCATTTTTG TTTTACAAAA ATGAAGCACA GATTCTTCGT 
           7861 TGGTAAAATA GCGCTTTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT 
           7921 GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTCTA CAAAATGAAG CACAGATGCT 
           7981 TCGTTGCT
          //
          

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