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          YIplac128

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: ATCC
          載體名稱: YIplac128
          質粒類型: 釀酒酵母表達載體;酵母整合型載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: lacZ
          載體大小: 4302 bp
          5' 測序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
          3' 測序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: LEU2
          表達菌株: 釀酒酵母、白色念珠菌
          備注: --
          產品目錄號: #87592
          穩定性: 穩表達
          組成型/誘導型: 誘導型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          YIplac128載體圖譜



          YIplac128 多克隆位點

          載體簡介

          載體序列

          LOCUS       YIplac128	4302 bp 	DNA	SYN
          DEFINITION  YIplac128
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..4302
                               /organism="YIplac128"
                               /mol_type="other DNA"
               promoter        142..171
                               /label="lac_promoter"
               misc_feature    185..207
                               /label="M13_pUC_rev_primer"
               promoter        206..224
                               /label="M13_reverse_primer"
               misc_feature    287..442
                               /label="lacZ_a"
               promoter        complement(290..306)
                               /label="M13_forward20_primer"
               misc_feature    complement(299..321)
                               /label="M13_pUC_fwd_primer"
               misc_feature    634..656
                               /label="pGEX_3_primer"
               gene            complement(797..1891)
                               /label="LEU2 (variant)"
                               /gene="LEU2 (variant)"
               CDS             complement(797..1903)
                               /label="ORF frame 3"
                               /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                               FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                               ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                               WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                               PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                               SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                               KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
               promoter        2431..2459
                               /label="AmpR_promoter"
                               /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                               FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                               ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                               WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                               PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                               SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                               KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
               gene            2501..3361
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                               FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                               ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                               WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                               PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                               SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                               KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
               CDS             2501..3361
                               /label="ORF frame 2"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               rep_origin      3516..4135
                               /label="pBR322_origin"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
             61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
            121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
            181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
            241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
            301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
            361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
            421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
            481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
            541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
            601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
            661 caccgtcatc accgaaacgc gcgagacgaa agggcctacc ctatgaacat attccatttt
            721 gtaatttcgt gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata
            781 aaaaaagaga atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga
            841 cttcggtggt actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct
            901 ttttaactgc atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca
            961 tcattgcagc agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag
           1021 cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca
           1081 aggacgcaga tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga
           1141 tatcaccaaa catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta
           1201 ggatcatggc ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct
           1261 tgatggtttc ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat
           1321 ccaaggacca aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg
           1381 tgattctttg cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat
           1441 cgtcttcctt tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt
           1501 cagtaccttt agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa
           1561 agttacatgg tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt
           1621 gttcaggtct aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg
           1681 catcaacctt cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga
           1741 tagcagcacc accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag
           1801 aaatagcttt aagaacctta atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg
           1861 gcaaaacgac gatcttctta ggggcagaca taggggcaga cattagaatg gtatatcctt
           1921 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat
           1981 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat
           2041 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc
           2101 ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac
           2161 ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
           2221 tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat
           2281 cttacgatac ctgagtattc ccacagttgg cctcgtgata cgcctatttt tataggttaa
           2341 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
           2401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
           2461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
           2521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
           2581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
           2641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
           2701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
           2761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
           2821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
           2881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
           2941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
           3001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
           3061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
           3121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
           3181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
           3241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
           3301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
           3361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
           3421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
           3481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
           3541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
           3601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
           3661 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
           3721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
           3781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
           3841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
           3901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
           3961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
           4021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
           4081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
           4141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
           4201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
           4261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
          //
          

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