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          pkk223-3

          價格:900元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: PL-Pharmacia
          載體名稱: pkk223-3
          質粒類型: 大腸桿菌表達載體
          高拷貝/低拷貝: --
          啟動子: --
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 4586 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: Ampicillin
          篩選標記: --
          備注: --
          產品目錄號: --
          穩定性: --
          組成型: --
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pkk223-3載體圖譜

          載體簡介

          pKK223-3 (1) contains the strong tac promoter upstream from the
          multiple cloning site and the strong rrnB ribosomal terminator downstream
          for control of protein expression (2-4).
          Induction: tac promoter is inducible with 1-5 mM IPTG. Promoter is
          very strong; even uninduced cells may show a low level of expression.
          Expression: Genes containing a ribosome-binding site and ATG can be
          inserted into any unique site in the MCS for expression. The ribosomebinding
          site on the plasmid can be utilised to express inserts if the ATG
          start codon of the insert is within 5-13 bp from the provided ribosomebindingsite (5, 6).
          Transcription terminators: rrnB transcription terminators stabilize the
          plasmid by inhibiting read-through transcription initiated from the tac
          promoter in the parent plasmid.
          
          Host(s): lac Iq strains; E. coli JM105 is recommended.
          
          Selectable marker(s): Plasmid confers resistance to 100 μg/ml ampicillin.
          Amplification: Recommended.
          
          Control Regions: Expression control region: tac promoter: -10: 50-44; -
          35: 72-67; Ribosome binding site: 11. MCS: 4552-4587. rrnB operon
          region: 5S rRNA region: 4471-4352; rrnB T1 terminator: 4348-4305;
          rrnB T2 terminator: 4173-4146. b-lactamase gene region: Promoter: -10:
          4082-4077; -35: 4105-4100; Start codon (ATG): 4035; Stop codon
          (TAA): 3178. Plasmid replication region: Site of replication initiation:
          2417-2415; Region necessary for replication: 3111-2415.
          

          載體序列

          LOCUS       pKK223-3	4586 bp 	DNA	SYN
          DEFINITION  pKK223-3
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..4586
                               /organism="pKK223-3"
                               /mol_type="other DNA"
               misc_feature    complement(9..31)
                               /label="M13_pUC_rev_primer"
               promoter        complement(43..71)
                               /label="tac_promoter"
               gene            268..1158
                               /label="tet (300 - 1190)"
                               /gene="tet (300 - 1190)"
               misc_feature    complement(320..339)
                               /label="pBRrevBam_primer"
               CDS             373..1158
                               /label="ORF frame 1"
                               /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                               LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG
                               EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA
                               FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL
                               IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
               CDS             complement(505..963)
                               /label="ORF frame 3"
                               /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                               RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                               CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
               misc_feature    1799..1990
                               /label="ROP"
                               /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                               RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                               CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
               misc_feature    complement(1984..2006)
                               /label="pGEX_3_primer"
                               /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                               RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                               CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
               gene            complement(3177..4037)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                               RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                               CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
               CDS             complement(3177..4037)
                               /label="ORF frame 1"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(4079..4107)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               terminator      complement(4148..4175)
                               /label="rrnB_T2_terminator"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               terminator      complement(4307..4350)
                               /label="rrnB_T1_terminator"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               terminator      complement(4316..4473)
                               /label="rrnB_terminator"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               misc_feature    4506..4523
                               /label="pBAD_rev_primer"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               misc_feature    4506..4523
                               /label="pTrcHis_rev_primer"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 ttctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagccgatg
             61 attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa
            121 aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac
            181 ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
            241 gcagtcgata agctccggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
            301 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
            361 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
            421 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
            481 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg
            541 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
            601 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
            661 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
            721 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
            781 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
            841 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
            901 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
            961 catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga
           1021 ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg
           1081 attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
           1141 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa
           1201 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
           1261 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg
           1321 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
           1381 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
           1441 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
           1501 cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg
           1561 caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt
           1621 gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac
           1681 aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc
           1741 gtttcatcgg tatcattacc cccatgaaca gaaattcccc cttacacgga ggcatcaagt
           1801 gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
           1861 gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
           1921 tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
           1981 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
           2041 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
           2101 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
           2161 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
           2221 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
           2281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
           2341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
           2401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
           2461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
           2521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
           2581 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
           2641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
           2701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
           2761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
           2821 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
           2881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
           2941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
           3001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
           3061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
           3121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
           3181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
           3241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
           3301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
           3361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
           3421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
           3481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
           3541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
           3601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
           3661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
           3721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
           3781 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
           3841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
           3901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
           3961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
           4021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
           4081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgtag
           4141 aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta
           4201 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
           4261 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
           4321 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
           4381 ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
           4441 caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc
           4501 gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa cagaagcttg
           4561 gctgcaggtc gacggatccc cgggaa
          //
          

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