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          pET-49b(+)

          價格:800元

          聯系方式:I47-825O-882O

          買家導航

          pET49b載體基本信息

          出品公司: EMD Biosciences (Novagen)
          別名: pET49b, pET 49b
          質粒類型: 大腸桿菌蛋白表達
          表達水平:
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 5926 bp
          5' 測序引物: T7或者pGEX 5'
          5' 測序引物序列:   T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX 5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
          3' 測序引物: T7t
          3' 測序引物序列:   T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
          載體標簽: N-GST, N-His, N-HRV 3C, C-S, C-Thrombin
          載體抗性: Kanamycin
          表達宿主菌: BL21(DE3) 、 Rosetta2(DE3)、C43(DE3)
          備注:
          Same as pET47 but also has Nterm GST Tag; contains HRV 3C Protease cleavage site for fusion tag removal at low temperatures; Cterm thrombin cleavage site.
          產品目錄號: 71463-3
          穩定性: 瞬時表達 Transient
          組成型: 組成型 Constitutive
          病毒/非病毒: 非病毒

          pET49b載體質粒圖譜和多克隆位點信息

          pET-49b載體圖譜



          pET-49b多克隆位點

          pET-49b載體特征

          Feature Name Start End  
          T7_terminator 120 1
          T7_Terminal_primer 69 87
          S15 212 168
          6xHIS 359 342
          GST (variant) 1088 438
          T7_transl_en_RBS 1113 1097
          lacO 1158 1131
          T7_promoter 1176 1158
          tet (300 - 563) 1212 1475
          pBRrevBam_primer 1283 1264
          lacI 1558 2649
          ROP 3221 3412
          pGEX_3_primer 3428 3406
          pBR322_origin 4446 3827
          KanR2 4552 5367
          f1_origin 5898 5592
          ORF Start End  
          ORF frame 3 1088 141
          ORF frame 3 1089 1817
          ORF frame 1 1690 2649
          ORF frame 1 4552 5367
          Enzyme Name Cut
          PacI 141
          XhoI 147
          SacI 238
          NotI 242
          EagI 242
          HindIII 249
          SalI 255
          AatII 266
          AscI 270
          StuI 280
          EcoRI 289
          BamHI 295
          KpnI 306
          XmaI 306
          SmaI 308
          SacII 338
          SpeI 417
          MscI 880
          NdeI 1087
          XbaI 1125
          ApaI 2128
          HpaI 2423
          NruI 4640

          pET49b載體簡介

          The pET-49b(+) vector carries N-terminal GST•Tag™ and His•Tag? coding sequences followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specifi c for cleavage of the sequence LEVLFQ↓GP , and is active at low temperatures . pET-49b(+) also contains an optional C-terminal thrombin recognition site followed by an S•Tag™ coding sequence. Unique restriction .sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with the helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the AS S•Tag 18mer Primer (Cat. No. 71262-3). 
          
          pET Vectors 選擇指南

          pET49b載體序列

          LOCUS       pET-49b(+)              5926 bp    DNA     circular     11-SEP-2008
          DEFINITION  pET-49b(+).
          SOURCE      
            ORGANISM  
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     ORIGDB|GenBank
          COMMENT     VNTAUTHORNAME|biofeng.com|
          COMMENT     VNTOAUTHORAD1|Novagen Inc.|
          COMMENT     VNTREPLTYPE|Plasmid
          COMMENT     VNTEXTCHREPL|Bacteria
          FEATURES             Location/Qualifiers
               rep_origin      complement(5467..5914)
                               /vntifkey="33"
                               /label=f1\ori
               misc_RNA        complement(3843..4398)
                               /vntifkey="53"
                               /label=RNA\II
               rep_origin      complement(3843..3843)
                               /vntifkey="33"
                               /label=ColE1,\pBR\ori
               misc_RNA        4283..4390
                               /vntifkey="53"
                               /label=RNA\I
               CDS             3221..3412
                               /vntifkey="4"
                               /label=ROP\gene
               CDS             1567..2649
                               /vntifkey="4"
                               /label=lacI
                               /note="lac repressor"
               promoter        complement(1160..1176)
                               /vntifkey="30"
                               /label=T7\promoter
               protein_bind    complement(1135..1155)
                               /vntifkey="31"
                               /label=lacO
               CDS             complement(429..1088)
                               /vntifkey="4"
                               /label=GST
               protein_bind    complement(342..359)
                               /vntifkey="31"
                               /label=His-Tag
               terminator      complement(26..73)
                               /vntifkey="43"
                               /label=T7\terminator
               CDS             4552..5367
                               /vntifkey="4"
                               /label=Kan
               misc_feature    complement(213..230)
                               /vntifkey="21"
                               /label=Thrombin\site
               protein_bind    complement(168..212)
                               /vntifkey="31"
                               /label=S-Tag
               protein_bind    complement(310..332)
                               /vntifkey="31"
                               /label=3c
               primer          complement(300..336)
                               /vntifkey="27"
                               /label=S-SacII/Bam\+SanDI\-dcm
               primer          complement(374..421)
                               /vntifkey="27"
                               /label=S-Tag\Spacer-Spe\I
          BASE COUNT     1485 a      1486 c      1520 g      1435 t
          ORIGIN
                  1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
                 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
                121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc
                181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct
                241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc
                301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga
                361 ccagaaccac tagatggagt aggagtagga ggattgttat tagaaccgcc accaccacta
                421 gttgaaccat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg ccagccctgc
                481 aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg gatagcttca
                541 atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg gtccatgtat
                601 aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg atcaccattt
                661 aaatatgttt tatgacataa acgatcttcg aacattttca gcatttcagg tagcttgcta
                721 agaaaatcaa ctttgagagt ttcaaagtct ttactatatg caattctcga aacaccgtat
                781 ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt tggacaacca
                841 cccaacatgt tgtgcttgtc agctatataa cgtatgatgg ccatagactg tgttaattta
                901 acatcaccat caatataata aggaagattg ggaaactcca aacccaattc aaactttttg
                961 tttcgccatt tatcaccttc atcgcgctca tacaaatgct cttcatattt ttcttcaaga
               1021 tattccaaaa gaagtcgagt gggttgcaca aggcccttaa ttttccaata acctagtata
               1081 ggggacatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
               1141 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct
               1201 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
               1261 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
               1321 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
               1381 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
               1441 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa
               1501 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg
               1561 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
               1621 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
               1681 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc
               1741 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa
               1801 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg
               1861 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc
               1921 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct
               1981 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt
               2041 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt
               2101 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg
               2161 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc
               2221 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt
               2281 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc
               2341 gagtccgggc tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac
               2401 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa
               2461 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
               2521 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct
               2581 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
               2641 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg
               2701 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
               2761 tagcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
               2821 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
               2881 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
               2941 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct
               3001 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt
               3061 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg
               3121 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat
               3181 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac
               3241 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa
               3301 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga
               3361 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
               3421 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
               3481 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
               3541 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
               3601 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
               3661 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
               3721 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
               3781 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
               3841 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
               3901 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
               3961 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
               4021 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
               4081 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
               4141 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
               4201 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
               4261 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
               4321 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
               4381 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
               4441 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
               4501 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
               4561 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta
               4621 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
               4681 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
               4741 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
               4801 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc
               4861 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
               4921 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagt
               4981 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
               5041 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
               5101 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
               5161 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
               5221 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
               5281 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
               5341 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg
               5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
               5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
               5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
               5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
               5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
               5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
               5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
               5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
               5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca
          //

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