免费高清视频 成人免费视频 成年在线视频免费观看视频

    <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

      1. <pre id="q7rs8"></pre>
        <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
        <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

          pGL4.80[hRlucP/Neo]

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          pGL4.80載體基本信息

          出品公司: Promega
          載體名稱: pGL4.80 ; pGL4.80[hRlucP/Neo]
          質粒類型: 信號通路報告載體;哺乳動物載體;海腎熒光素酶報告載體
          高拷貝/低拷貝: --
          克隆方法: 限制性內切酶,多克隆位點
          啟動子:
          載體大小: 5010 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: hRlucP
          載體抗性: 氨芐青霉素
          篩選標記: Neomycin
          克隆菌株: TOP10等常規菌株
          宿主細胞(系): HEK293等
          備注: pGL4.80[hRlucP/Neo]載體是信號通路報告載體;無啟動子;含海腎熒光素酶報告基因hRlucP。
          產品目錄號: E6981
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          pGL4.80載體質粒圖譜和多克隆位點信息

          pGL4.80載體圖譜



          pGL4.80 多克隆位點

          pGL4.80 載體特征

          pGL4.80載體簡介

          pGL4.80載體簡介
          The pGL4.80[hRlucP/Neo] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.80[hRlucP/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucP reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with a greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
          

          pGL4.80載體序列

          LOCUS       DQ188844                5010 bp    DNA     circular SYN 16-NOV-2005
          DEFINITION  Synthetic Renilla luciferase reporter vector pGL4.80[hRlucP/Neo],
                      complete sequence.
          ACCESSION   DQ188844
          VERSION     DQ188844.1  GI:76364306
          KEYWORDS    .
          SOURCE      Renilla luciferase reporter vector pGL4.80[hRlucP/Neo]
            ORGANISM  Renilla luciferase reporter vector pGL4.80[hRlucP/Neo]
                      other sequences; artificial sequences; vectors.
          REFERENCE   1  (bases 1 to 5010)
            AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
            TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
                      Corporation
            JOURNAL   Unpublished
          REFERENCE   2  (bases 1 to 5010)
            AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
            TITLE     Direct Submission
            JOURNAL   Submitted (31-AUG-2005) Scientific Communications, Promega
                      Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
          FEATURES             Location/Qualifiers
               source          1..5010
                               /organism="Renilla luciferase reporter vector
                               pGL4.80[hRlucP/Neo]"
                               /mol_type="other DNA"
                               /db_xref="taxon:346091"
               misc_feature    1..70
                               /note="multiple cloning region"
               CDS             100..1158
                               /note="synthetic Renilla luciferase; contains hPEST
                               destabilization sequence"
                               /codon_start=1
                               /product="hRlucP"
                               /protein_id="ABA41674.1"
                               /db_xref="GI:76364307"
                               /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                               NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                               AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                               EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                               WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                               EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
                               ESGMDRHPAACASARINV"
               regulatory      1198..1419
                               /regulatory_class="polyA_signal_sequence"
                               /note="SV40 late poly(A) signal"
               regulatory      1467..1885
                               /regulatory_class="promoter"
                               /note="SV40 early enhancer/promoter"
               CDS             1910..2704
                               /note="neomycin resistance"
                               /codon_start=1
                               /product="neomycin phosphotransferase"
                               /protein_id="ABA41675.1"
                               /db_xref="GI:76364308"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
               regulatory      2729..2777
                               /regulatory_class="polyA_signal_sequence"
                               /note="synthetic poly(A) signal"
               primer_bind     2844..2863
                               /note="Reporter Vector primer 4 (RVprimer4) binding
                               region"
               rep_origin      3101
                               /note="ColEI-derived plasmid replication origin"
               gene            complement(3892..4752)
                               /gene="bla"
               CDS             complement(3892..4752)
                               /gene="bla"
                               /note="ampicillin resistance"
                               /codon_start=1
                               /product="beta-lactamase"
                               /protein_id="ABA41676.1"
                               /db_xref="GI:76364309"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW"
               regulatory      4857..5010
                               /regulatory_class="polyA_signal_sequence"
                               /note="synthetic poly(A) signal/transcriptional pause
                               site"
               primer_bind     4959..4978
                               /note="Reporter Vector primer 3 (RVprimer3) binding
                               region"
          ORIGIN      
                  1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
                 61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
                121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
                181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
                241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
                301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
                361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
                421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
                481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
                541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
                601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
                661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
                721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
                781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
                841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
                901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
                961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
               1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
               1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
               1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
               1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
               1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
               1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
               1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
               1441 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
               1501 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
               1561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
               1621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
               1681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
               1741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
               1801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
               1861 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgatcgaaca
               1921 agacggcctc catgctggca gtcccgcagc ttgggtcgaa cgcttgttcg ggtacgactg
               1981 ggcccagcag accatcggat gtagcgatgc ggccgtgttc cgtctaagcg ctcaaggccg
               2041 gcccgtgctg ttcgtgaaga ccgacctgag cggcgccctg aacgagcttc aagacgaggc
               2101 tgcccgcctg agctggctgg ccaccaccgg cgtaccctgc gccgctgtgt tggatgttgt
               2161 gaccgaagcc ggccgggact ggctgctgct gggcgaggtc cctggccagg atctgctgag
               2221 cagccacctt gcccccgctg agaaggtttc tatcatggcc gatgcaatgc ggcgcctgca
               2281 caccctggac cccgctacct gccccttcga ccaccaggct aagcatcgga tcgagcgtgc
               2341 tcggacccgc atggaggccg gcctggtgga ccaggacgac ctggacgagg agcatcaggg
               2401 cctggccccc gctgaactgt tcgcccgact gaaagcccgc atgccggacg gtgaggacct
               2461 ggttgtcaca cacggagatg cctgcctccc taacatcatg gtcgagaatg gccgcttctc
               2521 cggcttcatc gactgcggtc gcctaggagt tgccgaccgc taccaggaca tcgccctggc
               2581 cacccgcgac atcgctgagg agcttggcgg cgagtgggcc gaccgcttct tagtcttgta
               2641 cggcatcgca gctcccgaca gccagcgcat cgccttctac cgcttgctcg acgagttctt
               2701 ttaatgatct agaaccggtc atggccgcaa taaaatatct ttattttcat tacatctgtg
               2761 tgttggtttt ttgtgtgttc gaactagatg ctgtcgaccg atgcccttga gagccttcaa
               2821 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
               2881 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
               2941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
               3001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
               3061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
               3121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
               3181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
               3241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
               3301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
               3361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
               3421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
               3481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
               3541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
               3601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
               3661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
               3721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
               3781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
               3841 atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg
               3901 cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg
               3961 actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc
               4021 aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc
               4081 agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag
               4141 ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc
               4201 cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg
               4261 ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc
               4321 cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat
               4381 ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg
               4441 cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc
               4501 ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg
               4561 gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat
               4621 atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg
               4681 gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg
               4741 ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta
               4801 cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata
               4861 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
               4921 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
               4981 gtgcaagtgc aggtgccaga acatttctct
          //
          

          pGL4.80載體質粒圖譜pdf版和相關資料下載

          pGL4.80載體應用舉例

          分享到:

          全部載體分類

          相關載體

          相關文章

          相關問答

          Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng
          歡迎您的訪問和咨詢....
            <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

              1. <pre id="q7rs8"></pre>
                <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
                <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

                  免费高清视频 成人免费视频 成年在线视频免费观看视频

                  品牌简介

                  免费高清视频 成人免费视频 成年在线视频免费观看视频