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          pCMV-SPORT1

          價格:2000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Invitrogen
          載體名稱: pCMV-SPORT1
          質粒類型: 哺乳動物表達載體;cDNA克隆載體;I.M.A.G.E.載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: CMV
          載體大小: 4380 bp
          5' 測序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
          3' 測序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: --
          克隆菌株: DH5α
          宿主細胞(系): 哺乳動物細胞
          備注: 用于cDNA文庫的構建
          產品目錄號: --
          穩定性: 瞬表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pCMV-SPORT1載體圖譜
          pCMV-SPORT1多克隆位點

          pCMV-SPORT1載體特征

          載體簡介

          載體序列

          LOCUS       Exported                4380 bp ds-DNA     circular SYN 28-FEB-2013
          DEFINITION  Vector for cloning and transient mammalian cell expression of cDNAs.
          ACCESSION   .
          VERSION     .
          KEYWORDS    pCMV SPORT
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 4380)
            AUTHORS   I.M.A.G.E. Consortium
          FEATURES             Location/Qualifiers
               source          1..4380
                               /organism="synthetic DNA construct"
                               /lab_host="Mammalian Cells"
                               /mol_type="other DNA"
               polyA_signal    125..259
                               /note="SV40 poly(A) signal"
                               /note="SV40 polyadenylation signal"
               promoter        646..664
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               misc_feature    682..779
                               /note="MCS"
                               /note="multiple cloning site"
               promoter        complement(787..805)
                               /note="SP6 promoter"
                               /note="promoter for bacteriophage SP6 RNA polymerase"
               promoter        complement(921..1124)
                               /note="CMV promoter"
                               /note="human cytomegalovirus (CMV) immediate early 
                               promoter"
               enhancer        1125..1428
                               /note="CMV enhancer"
                               /note="human cytomegalovirus immediate early enhancer"
               protein_bind    1458..1474
                               /bound_moiety="lac repressor encoded by lacI"
                               /note="lac operator"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               promoter        complement(1482..1513)
                               /note="lac UV5 promoter"
                               /note="E. coli lac promoter with an ""up"" mutation"
               rep_origin      complement(1975..2563)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               protein_bind    2641..2674
                               /bound_moiety="Cre recombinase"
                               /note="loxP"
                               /note="Cre-mediated recombination occurs in the 8-bp core 
                               sequence (GCATACAT)."
               CDS             complement(2776..3636)
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
               rep_origin      complement(3924..4379)
                               /direction=LEFT
                               /note="f1 ori"
                               /note="f1 bacteriophage origin of replication; arrow 
                               indicates direction of (+) strand synthesis"
          ORIGIN
                  1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
                 61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
                121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
                181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
                241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
                301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
                361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
                421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
                481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
                541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
                601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcttataata cgactcacta
                661 tagggagaga gctatgacgt cgcatgcacg cgtaagcttg ggcccctcga gggatcctct
                721 agagcggccg ccgactagtg agctcgtcga cccgggaatt ccggaccggt acctgcaggc
                781 gtaccttcta tagtgtcacc taaatagctt tttgcaaaag cctaggctag agtccggagg
                841 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
                901 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg
                961 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
               1021 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
               1081 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
               1141 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
               1201 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
               1261 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
               1321 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
               1381 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgac ctgcacgatg
               1441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggaagc
               1501 tataaagtgt aaagcctggg gtgcctaatg agtgaaaggg cctcgtatac gcctattttt
               1561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
               1621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
               1681 gagacaataa ccctgataaa tgcttcaata atattgaaaa acgcgcgaat tgcaagctct
               1741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
               1801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
               1861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
               1921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
               1981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
               2041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
               2101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
               2161 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
               2221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
               2281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
               2341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
               2401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
               2461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
               2521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
               2581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc
               2641 ataacttcgt atagcataca ttatacgaag ttatggcatg agattatcaa aaaggatctt
               2701 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
               2761 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
               2821 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
               2881 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
               2941 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
               3001 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
               3061 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
               3121 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
               3181 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
               3241 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
               3301 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
               3361 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
               3421 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
               3481 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
               3541 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
               3601 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
               3661 aagcatttat cagggttatt gtctcatgcc aggggtgggc acacatattt gataccagcg
               3721 atccctacac agcacataat tcaatgcgac ttccctctat cgcacatctt agacctttat
               3781 tctccctcca gcacacatcg aagctgccga gcaagccgtt ctcaccagtc caagacctgg
               3841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
               3901 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt
               3961 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
               4021 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
               4081 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
               4141 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
               4201 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
               4261 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
               4321 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
          //
          
          

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