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          pCMV-3Tag-4A

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Agilent
          載體名稱: pCMV-3Tag-4A
          質粒類型: 哺乳動物細胞載體
          高拷貝/低拷貝: --
          克隆方法: --
          啟動子: --
          載體大小: 4233 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: Myc
          載體抗性: 卡那霉素
          篩選標記: 新霉素
          克隆菌株: --
          宿主細胞(系): --
          備注: 哺乳動物C末端3xMyc表位蛋白的表達載體
          產品目錄號: --
          穩定性: --
          組成型/誘導型: --
          病毒/非病毒: --

          載體質粒圖譜和多克隆位點信息

          pCMV-3Tag-4A載體圖譜

          載體簡介

          載體序列

          LOCUS       pCMV-3Tag-4A                4233 bp ds-DNA     circular SYN 28-NOV-2013
          DEFINITION  Mammalian expression vector for tagging proteins with a C-terminal 
                      3xMyc epitope. For other reading frames, use pCMV-3Tag-4B or 
                      pCMV-3Tag-4C.
          ACCESSION   .
          VERSION     .
          KEYWORDS    pCMV-3Tag-4A
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 4233)
            AUTHORS   Agilent Technologies
            TITLE     Direct Submission
            JOURNAL   Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
                      http://www.snapgene.com
          COMMENT     The cloned gene must include a Kozak sequence and start codon.
          FEATURES             Location/Qualifiers
               source          1..4233
                               /organism="synthetic DNA construct"
                               /lab_host="Mammalian Cells"
                               /mol_type="other DNA"
               enhancer        66..370
                               /note="CMV enhancer"
                               /note="human cytomegalovirus immediate early enhancer"
               promoter        371..574
                               /note="CMV promoter"
                               /note="human cytomegalovirus (CMV) immediate early 
                               promoter"
               promoter        620..638
                               /note="T3 promoter"
                               /note="promoter for bacteriophage T3 RNA polymerase"
               misc_feature    651..743
                               /note="MCS"
                               /note="multiple cloning site"
               CDS             744..833
                               /codon_start=1
                               /product="3 tandem Myc epitope tags"
                               /note="3xMyc"
                               /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
               misc_feature    850..860
                               /note="stop codons"
                               /note="stop codons in all three reading frames"
               promoter        complement(888..906)
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               polyA_signal    941..1148
                               /note="bGH poly(A) signal"
                               /note="bovine growth hormone polyadenylation signal"
               rep_origin      complement(1155..1610)
                               /direction=LEFT
                               /note="f1 ori"
                               /note="f1 bacteriophage origin of replication; arrow 
                               indicates direction of (+) strand synthesis"
               promoter        1637..1739
                               /gene="bla"
                               /note="AmpR promoter"
               promoter        1743..2100
                               /note="SV40 promoter"
                               /note="SV40 enhancer and early promoter"
               CDS             2135..2929
                               /codon_start=1
                               /gene="aph(3')-II (or nptII)"
                               /product="aminoglycoside phosphotransferase from Tn5"
                               /note="NeoR/KanR"
                               /note="confers resistance to neomycin, kanamycin, and G418 
                               (Geneticin)"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                               VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                               SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                               GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                               LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
               misc_feature    3161..3208
                               /note="HSV TK poly(A) signal"
                               /note="herpesvirus thymidine kinase polyadenylation signal"
               rep_origin      3537..4125
                               /direction=RIGHT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
          ORIGIN
                  1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
                 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
                121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
                181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
                241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
                301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
                361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
                421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
                481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
                541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
                601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
                661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
                721 ttatcgatac cgtcgacctc gaggagcaga aactcatctc tgaagaagat ctggaacaaa
                781 agttgatttc agaagaagat ctggaacaga agctcatctc tgaggaagat ctgtaagggc
                841 ccggtacctt aattaattaa ggtaccaggt aagtgtaccc aattcgccct atagtgagtc
                901 gtattacaat tcactcgatc ggctcgctga tcagcctcga ctgtgccttc tagttgccag
                961 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
               1021 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
               1081 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
               1141 gctggggaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
               1201 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
               1261 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
               1321 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
               1381 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
               1441 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
               1501 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
               1561 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
               1621 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
               1681 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa
               1741 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
               1801 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
               1861 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
               1921 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
               1981 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
               2041 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
               2101 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
               2161 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
               2221 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
               2281 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
               2341 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
               2401 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
               2461 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
               2521 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
               2581 agccggtctt gtcgatcagg atgatctgga cgaagaacat caggggctcg cgccagccga
               2641 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
               2701 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
               2761 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
               2821 tgaagaactt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
               2881 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
               2941 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
               3001 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
               3061 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
               3121 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
               3181 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
               3241 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
               3301 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
               3361 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
               3421 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
               3481 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
               3541 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
               3601 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
               3661 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
               3721 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
               3781 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
               3841 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
               3901 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
               3961 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
               4021 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
               4081 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
               4141 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
               4201 tcccctgatt ctgtggataa ccgtattacc gcc
          //
          
          
          

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