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          pBK-RSV

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Agilent
          載體名稱: pBK-RSV Phagemid vector
          質粒類型: 真核原核雙系統表達載體;克隆載體;噬粒載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 多克隆位點,限制性內切酶
          啟動子: 原核啟動子lac;真核啟動子RSV
          載體大小: 4452 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 卡那霉素Kanamycin
          篩選標記: 新霉素Neomycin
          克隆宿主菌: XL1-Blue
          備注: 原核細胞的陽性克隆可用藍白斑篩選法。
          貨號: 已停產
          組成型/誘導型: 真核細胞組成型表達,原核細胞誘導型表達
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pBK-RSV載體圖譜



          載體簡介

          載體序列

          LOCUS       pBK-RSV	4452 bp 	DNA	SYN
          DEFINITION  pBK-RSV
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..4452
                               /organism="pBK-RSV"
                               /mol_type="other DNA"
               rep_origin      24..330
                               /label="f1_origin"
               misc_feature    complement(480..499)
                               /label="EBV_rev_primer"
               misc_feature    complement(748..807)
                               /label="SV40_3_splice"
               misc_feature    complement(823..971)
                               /label="lacZ_a"
               misc_feature    937..959
                               /label="M13_pUC_fwd_primer"
               promoter        952..968
                               /label="M13_forward20_primer"
               promoter        975..993
                               /label="T7_promoter"
               promoter        complement(1139..1158)
                               /label="T3_promoter"
               misc_feature    complement(1192..1214)
                               /label="M13_pUC_rev_primer"
               promoter        complement(1308..1829)
                               /label="RSV_promoter"
               misc_feature    complement(1338..1356)
                               /label="pREP_fwd_primer"
               CDS             1413..1871
                               /label="ORF frame 3"
                               /translation="MSDPSVAFLIRHDRTTLLPPIGMHGAFSLLVRHVANSSLPCCKT
                               TRVLHKTTFPPPYAKAKLLYPEGTPNRVQPKPRFSPKHTLVPSDTRISGPYIAKQRKT
                               PNPKQILHAIVGQALPCCSLNFARALLSDLQHTSREQMHGGNTVIHRIRG*"
               rep_origin      complement(1929..2548)
                               /label="pBR322_origin"
                               /translation="MSDPSVAFLIRHDRTTLLPPIGMHGAFSLLVRHVANSSLPCCKT
                               TRVLHKTTFPPPYAKAKLLYPEGTPNRVQPKPRFSPKHTLVPSDTRISGPYIAKQRKT
                               PNPKQILHAIVGQALPCCSLNFARALLSDLQHTSREQMHGGNTVIHRIRG*"
               terminator      complement(2696..2965)
                               /label="TK_PA_terminator"
                               /translation="MSDPSVAFLIRHDRTTLLPPIGMHGAFSLLVRHVANSSLPCCKT
                               TRVLHKTTFPPPYAKAKLLYPEGTPNRVQPKPRFSPKHTLVPSDTRISGPYIAKQRKT
                               PNPKQILHAIVGQALPCCSLNFARALLSDLQHTSREQMHGGNTVIHRIRG*"
               CDS             3089..3625
                               /label="ORF frame 2"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               CDS             complement(3140..3934)
                               /label="ORF frame 3"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               gene            complement(3143..3922)
                               /label="NeoR/KanR (variant)"
                               /gene="NeoR/KanR (variant)"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               promoter        complement(4017..4285)
                               /label="SV40_promoter"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               misc_feature    complement(4037..4056)
                               /label="SV40pro_F_primer"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               rep_origin      complement(4041..4118)
                               /label="SV40_origin"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               misc_feature    4088..4303
                               /label="SV40_enhancer"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               misc_feature    4283..4303
                               /label="pBABE_3_primer"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               promoter        complement(4369..4397)
                               /label="AmpR_promoter"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
          ORIGIN
              1 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG
             61 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC
            121 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC
            181 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA
            241 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA
            301 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG
            361 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
            421 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTACGCGTTA AGATACATTG
            481 ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT
            541 GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA
            601 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
            661 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCATGA ACAGACTGTG AGGACTGAGG
            721 GGCCTGAAAT GAGCCTTGGG ACTGTGAATC TAAAATACAC AAACAATTAG AATCAGTAGT
            781 TTAACACATT ATACACTTAA AAATTGGATC TCCATTCGCC ATTCAGGCTG CGCAACTGTT
            841 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG
            901 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
            961 CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGGTACACTT ACCTGGTACC
           1021 CCACCCGGGT GGAAAATCGA TGGGCCCGCG GCCGCTCTAG AAGTACTCTC GAGAAGCTTT
           1081 TTGAATTCTT TGGATCCACT AGTGTCGACC TGCAGGCGCG CGAGCTCCAG CTTTTGTTCC
           1141 CTTTAGTGAG GGTTAATTTC GAGCTTGGCG TAATCAAGGT CATAGCTGTT TCCTGTGTGA
           1201 AATTGTTATC CGCTCACAAT TCCACACAAT ATACGAGCCG GAAGTATAAA GTGTAAAGCC
           1261 TGGGGTGCCT AATGAGTGAG CTAACTCACA GTAATTGCGG CTAGCCAGGT GCACACCAAT
           1321 GTGGTGAATG GTCAAATGGC GTTTATTGTA TCGAGCTAGG CACTTAAATA CAATTATCTC
           1381 TGCAATGCGG TATTCAGTGG TTCGTCCAAT CCATGTCAGA CCCGTCTGTT GCCTTCCTAA
           1441 TAAGGCACGA TCGTACCACC TTACTTCCAC CAATCGGCAT GCACGGTGCT TTTTCTCTCC
           1501 TTGTAAGGCA TGTTGCTAAC TCATCGTTAC CATGTTGCAA GACTACAAGA GTATTGCATA
           1561 AGACTACATT TCCCCCTCCC TATGCAAAAG CGAAACTACT ATATCCTGAG GGGACTCCTA
           1621 ACCGCGTACA ACCGAAGCCC CGCTTTTCGC CTAAACACAC CCTAGTCCCC TCAGATACGC
           1681 GTATATCTGG CCCGTACATC GCGAAGCAGC GCAAAACGCC TAACCCTAAG CAGATTCTTC
           1741 ATGCAATTGT CGGTCAAGCC TTGCCTTGTT GTAGCTTAAA TTTTGCTCGC GCACTACTCA
           1801 GCGACCTCCA ACACACAAGC AGGGAGCAGA TGCATGGCGG TAATACGGTT ATCCACAGAA
           1861 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
           1921 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
           1981 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
           2041 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
           2101 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
           2161 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
           2221 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
           2281 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
           2341 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC
           2401 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
           2461 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
           2521 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
           2581 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
           2641 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG
           2701 GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG
           2761 TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA
           2821 TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG
           2881 TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC
           2941 TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG
           3001 CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG
           3061 AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT
           3121 TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT
           3181 GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA
           3241 GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGGTCC GCCACACCCA
           3301 GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC
           3361 AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG
           3421 CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA
           3481 GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG
           3541 GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT
           3601 TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA
           3661 GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG
           3721 TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT
           3781 CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG
           3841 AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG
           3901 GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT
           3961 GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC
           4021 TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC
           4081 GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT
           4141 ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG
           4201 GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT
           4261 GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG
           4321 CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
           4381 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
           4441 CCCGAAAAGT GC
          //
          
          

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