免费高清视频 成人免费视频 成年在线视频免费观看视频

    <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

      1. <pre id="q7rs8"></pre>
        <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
        <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

          pBI-CMV2

          價格:2200元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Clontech
          載體名稱: pBI-CMV2
          質粒類型: 哺乳細胞表達載體 ; 雙向表達載體;熒光報告載體
          高拷貝/低拷貝: 低拷貝
          啟動子: 弱啟動子minCMV
          克隆方法: 多克隆位點,限制性內切酶
          載體大小: 3822 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素(Ampicillin )
          篩選標記: --
          備注: pBI-CMV2表達綠色熒光蛋白AcGFP1
          產品目錄號: #631631
          穩定性: --
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pBI-CMV2載體圖譜



          pBI-CMV2多克隆位點

          載體簡介

          雙向啟動子載體pBI-CMV2表達綠色熒光蛋白AcGFP1 
          The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon. 
          eatures Constitutively express a protein of interest and a reporter protein (or two proteins of interest) 
          pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
          

          載體序列

          LOCUS       pBI-CMV2	3822 bp 	DNA	SYN
          DEFINITION  pBI-CMV2
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..3822
                               /organism="pBI-CMV2"
                               /mol_type="other DNA"
               promoter        4..568
                               /label="CMV_immearly_promoter"
               misc_feature    71..358
                               /label="CAG_enhancer"
               misc_feature    525..545
                               /label="CMV_fwd_primer"
               promoter        526..595
                               /label="CMV_promoter"
               gene            complement(1799..2659)
                               /label="Ampicillin"
                               /gene="Ampicillin"
               CDS             complement(1799..2659)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(2701..2729)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               CDS             2969..3520
                               /label="ORF frame 2"
                               /translation="MADYDPLDCSLRRSGPSLVQLIHAVGDGGGGHEAEVDHVIALLV
                               GVLGQGALGGQVVVIGQQHRAIADGGILLVVVGQLHAAILDVVADLEVHLDAILGLVG
                               HDVHIVGVVVVLHLIAQDVAILLEIGAGQLDAIHQGIALELHLGARLVVAVILEEDGA
                               LLDVALRHGALEEVVLLHVIGVA*"
               CDS             complement(3017..3736)
                               /label="ORF frame 3"
                               /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                               DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MIYFGFVTAAAITHGMDELYK*"
               gene            complement(3020..3733)
                               /label="AcGFP1"
                               /gene="AcGFP1"
                               /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                               DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MIYFGFVTAAAITHGMDELYK*"
               misc_feature    complement(3756..3780)
                               /label="LNCX_primer"
                               /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                               DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MIYFGFVTAAAITHGMDELYK*"
               misc_feature    complement(3763..3782)
                               /label="pCEP_fwd_primer"
                               /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                               LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                               DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                               GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                               MIYFGFVTAAAITHGMDELYK*"
          ORIGIN
              1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
             61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
            121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
            181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
            241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
            301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
            361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
            421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
            481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
            541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
            601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
            661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
            721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
            781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
            841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
            901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
            961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
           1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
           1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
           1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
           1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
           1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
           1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
           1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
           1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
           1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
           1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
           1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
           1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
           1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
           1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
           1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
           1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
           1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
           2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
           2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
           2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
           2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
           2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
           2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
           2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
           2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
           2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
           2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
           2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
           2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
           2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
           2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
           2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
           2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
           3001 caggagatct gggccctcac ttgtacagct catccatgcc gtgggtgatg gcggcggcgg
           3061 tcacgaagcc gaagtagatc atgtgatcgc gcttctcgtt ggggtccttg gacagggcgc
           3121 tctgggtgga caggtagtgg ttatcgggca gcagcacagg gccatcgccg atgggggtat
           3181 tctgctggta gtggtcggcc agctgcacgc tgccatcctc gatgttgtgg cggatcttga
           3241 agttcacctt gatgccattc ttggccttgt cggtcatgat gtacacattg tgggcgttgt
           3301 agttgtactc catcttattg cccaggatgt tgccatcctc cttgaaatcg gtgccggtca
           3361 gctcgatgcg attcaccagg gtatcgccct cgaacttcac ctcggcgcgc gacttgtagt
           3421 tgccgtcatc ctcgaagaag atggtgcgct cctggatgta gccctcaggc atggcgctct
           3481 tgaagaagtc gtgctgcttc atgtgatcgg ggtagcgtga gaagcactgc acgccgtagc
           3541 tcagggtggt caccagggtg ggccagggca caggcagctt gccggtggtg cagatgaact
           3601 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccgctcacg ctgaacttgt
           3661 ggccattcac atcgccattc agctcgatca ggatgggcac gatgccggtg aacagctcgg
           3721 cgcccttgct caccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct
           3781 ctgcttatat aggcctccca ccgtacacgc cacctcgaca ta
          //
          
          
          

          載體質粒圖譜pdf版和相關資料下載

          載體應用舉例

          分享到:

          全部載體分類

          相關載體

          相關文章

          相關問答

          Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng
          歡迎您的訪問和咨詢....
            <pre id="q7rs8"><label id="q7rs8"><menu id="q7rs8"></menu></label></pre>

              1. <pre id="q7rs8"></pre>
                <track id="q7rs8"><ruby id="q7rs8"></ruby></track>
                <acronym id="q7rs8"><label id="q7rs8"></label></acronym>

                  免费高清视频 成人免费视频 成年在线视频免费观看视频

                  品牌简介

                  免费高清视频 成人免费视频 成年在线视频免费观看视频