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          pET-53-DEST

          價格:1500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Novagen (EMD Millipore
          載體名稱: pET-53-DEST
          質粒類型: Gateway克隆載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: --
          啟動子: --
          載體大小: 6832 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素
          篩選標記: --
          克隆菌株: --
          宿主細胞(系): --
          備注: --
          產品目錄號: --
          穩定性: --
          組成型/誘導型: --
          病毒/非病毒: --

          載體質粒圖譜和多克隆位點信息

          pET-53-DEST載體圖譜

          載體簡介

          載體序列

          LOCUS       pET-53-DEST              6832 bp ds-DNA     circular SYN 23-OCT-2018
          DEFINITION  Gateway(R) Nova bacterial destination vector for expressing proteins
                      tagged at the C-terminus with Strep-Tag II.
          ACCESSION   .
          VERSION     .
          KEYWORDS    pET-53-DEST
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 6832)
            AUTHORS   MilliporeSigma (Novagen)
            TITLE     Direct Submission
            JOURNAL   Exported Monday, February 10, 2020 from SnapGene Viewer 3.1.4
                      http://www.snapgene.com
          FEATURES             Location/Qualifiers
               source          1..6832
                               /organism="synthetic DNA construct"
                               /lab_host="Escherichia coli"
                               /mol_type="other DNA"
               rep_origin      12..467
                               /direction=RIGHT
                               /note="f1 ori"
                               /note="f1 bacteriophage origin of replication; arrow 
                               indicates direction of (+) strand synthesis"
               CDS             complement(582..1442)
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
               promoter        complement(1443..1535)
                               /gene="bla"
                               /note="AmpR promoter"
               rep_origin      1616..2204
                               /direction=RIGHT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(2634..2825)
                               /codon_start=1
                               /gene="rop"
                               /product="Rop protein, which maintains plasmids at low copy
                               number"
                               /note="rop"
                               /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                               DELYRSCLARFGDDGENL"
               CDS             complement(3397..4479)
                               /codon_start=1
                               /gene="lacI"
                               /product="lac repressor"
                               /note="lacI"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                               /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                               NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                               EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                               EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                               MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                               YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                               ALADSLMQLARQVSRLESGQ"
               promoter        complement(4480..4557)
                               /gene="lacI"
                               /note="lacI promoter"
               promoter        4870..4888
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               protein_bind    4889..4913
                               /bound_moiety="lac repressor encoded by lacI"
                               /note="lac operator"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               RBS             4944..4949
                               /note="ribosome binding site"
               CDS             4957..4959
                               /codon_start=1
                               /product="start codon"
                               /note="ATG"
                               /translation="M"
               CDS             4963..4980
                               /codon_start=1
                               /product="6xHis affinity tag"
                               /note="6xHis"
                               /translation="HHHHHH"
               protein_bind    4984..5108
                               /gene="mutant version of attR"
                               /bound_moiety="LR Clonase(TM)"
                               /note="attR1"
                               /note="recombination site for the Gateway(R) LR reaction"
               promoter        5133..5163
                               /note="lac UV5 promoter"
                               /note="E. coli lac promoter with an ""up"" mutation"
               CDS             5217..5876
                               /codon_start=1
                               /gene="cat"
                               /product="chloramphenicol acetyltransferase"
                               /note="CmR"
                               /note="confers resistance to chloramphenicol"
                               /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                               KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                               LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                               DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
               CDS             6218..6523
                               /codon_start=1
                               /gene="ccdB"
                               /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                               /note="ccdB"
                               /note="Plasmids containing the ccdB gene cannot be 
                               propagated in standard E. coli strains."
                               /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                               VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
               protein_bind    complement(6564..6688)
                               /gene="mutant version of attR"
                               /bound_moiety="LR Clonase(TM)"
                               /note="attR2"
                               /note="recombination site for the Gateway(R) LR reaction"
               CDS             6696..6719
                               /codon_start=1
                               /product="peptide that binds Strep-Tactin, an engineered 
                               form of streptavidin"
                               /note="Strep-Tag II"
                               /translation="WSHPQFEK"
               terminator      6760..6807
                               /note="T7 terminator"
                               /note="transcription terminator for bacteriophage T7 RNA 
                               polymerase"
          ORIGIN
                  1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
                 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
                121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
                181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
                241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
                301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
                361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
                421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat
                481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
                541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
                601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
                661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
                721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
                781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
                841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
                901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
                961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
               1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
               1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
               1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
               1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
               1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
               1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
               1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
               1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg
               1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac
               1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
               1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
               1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
               1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
               1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
               1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
               1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
               1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
               2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
               2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
               2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
               2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
               2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
               2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
               2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
               2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
               2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
               2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
               2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
               2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
               2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
               2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
               2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
               2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
               3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
               3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
               3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
               3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
               3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc
               3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
               3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
               3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
               3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
               3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
               3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
               3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
               3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
               3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
               3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
               3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
               3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
               4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
               4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
               4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
               4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
               4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
               4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
               4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
               4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
               4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
               4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
               4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
               4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
               4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
               4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc
               4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
               4921 aaataatttt gtttaacttt aagaaggaga tataccatgg cacatcacca ccaccatcac
               4981 gtcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
               5041 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
               5101 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
               5161 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
               5221 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
               5281 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
               5341 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
               5401 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
               5461 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
               5521 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
               5581 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
               5641 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
               5701 atatggacaa cttcttcgcc cccgttttca ctatgggcaa atattatacg caaggcgaca
               5761 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
               5821 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
               5881 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
               5941 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
               6001 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
               6061 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
               6121 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
               6181 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
               6241 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
               6301 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
               6361 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
               6421 ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
               6481 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
               6541 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
               6601 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
               6661 ctcgttcagc tttcttgtac aaagtggttt ccgcttggag ccacccgcag ttcgaaaagt
               6721 aagtgattaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
               6781 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
          //
          
          
          

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