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          pENTR11-Dual

          價格:2100元

          聯系方式:I47-825O-882O

          買家導航

          pENTR11Dual載體基本信息

          出品公司: Invitrogen
          載體名稱: pENTR11
          質粒類型: Gateway載體;入門載體
          高拷貝/低拷貝: 高拷貝
          啟動子:
          克隆方法: 限制性內切酶;多克隆位點
          載體大小: 2744 bp
          5' 測序引物及序列: pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
          3' 測序引物及序列: pENTR-R: ATGGCTCATAACACCCCTTG
          載體標簽:
          載體抗性: 卡那霉素
          真核篩選標記:
          備注:
          pENTR11載體本身含有CCDb致死基因,能夠使得普通的DH5alpha等細菌死亡,必須使用DB3.1感受態細胞進行質粒擴增;

          構建Gateway系統入門克隆時需要使用ccdB敏感的 OmniMAX2 感受態細胞。
           
          產品目錄號: --
          病毒/非病毒: 非病毒

          pENTR11Dual載體質粒圖譜和多克隆位點信息

          pENTR11載體圖譜



          pENTR11多克隆位點

          pENTR11Dual載體簡介

          Gateway® pENTR™ 11 Dual Selection Vector Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: 
          • attL1 and attL2 sites for site-specific recombination of 
          
          the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression
          • Kozak consensus sequence for efficient translation initiation in eukaryotic systems
          
          • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
          
          • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli 
          
          • pUC origin for high-copy replication and maintenance of the plasmid in E. coli
          
          • Kanamycin resistance gene for selection in E. coli
          
          • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for negative selection and Chloramphenicol selection in E. coli.
          
          • Kanamycin resistance gene for selection in E. coli
          

          pENTR11Dual載體序列

          CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTAGCATGGATCTCGGGGACGTCTAA
          CTACTAAGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTT
          TCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAC
          GTTGTGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAACTAAGC
          AGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTTAGTTAGTTACTTAAGCT
          CGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGC
          TTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTTCGAAGGAGATAGAACCAATTCTCTAAGGAAAT
          ACTTAACCATGGTCGACTGGATCCGGTACCGAATTCGTCGACCTGCAGACTGGCTGTGTATAAGGGAGCC
          TGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCA
          GCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCAT
          CCCCGATATGCACCACCGGGTAAAGTTCACGGGGGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGG
          GATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTC
          TCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCAGCAAAAGAGCCGTTC
          ATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGAC
          GACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAAC
          TGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCG
          GGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCT
          TTTAGTAAGCCGGATCCTCTAGATTACGCCCCGCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAG
          CATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTG
          TCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTA
          AATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGG
          GAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCG
          TCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAA
          CACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCG
          GGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTA
          ATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTAC
          GATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCC
          TGAAAATCTCGACGGATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATAAAGTGTAAAGCCT
          GGGGTGCCTAATGCGGCCGCGAATTCGCGGCCGCACTCGAGATATCTAGACCCAGCTTTCTTGTACAAAG
          TTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAAT
          CATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAA
          AAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCAT
          ATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGG
          CTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTT
          GTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTG
          ACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCA
          CTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGA
          TGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGC
          GTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACG
          AGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTC
          AGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATT
          GATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGT
          TTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCA
          GTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACATTATTCAGATTGG
          GCCCCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGC
          GCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCT
          ACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG
          CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC
          CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAA
          GGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAA
          CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATC
          CGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTA
          TAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGC
          CTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT
          T
          

          pENTR11Dual載體質粒圖譜pdf版和相關資料下載

          pENTR11Dual載體應用舉例

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