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          pENTR1A

          價格:1200元

          聯系方式:I47-825O-882O

          買家導航

          pENTR1A載體基本信息

          出品公司: Invitrogen
          載體名稱: pENTR1A
          質粒類型: Gateway載體;入門載體
          高拷貝/低拷貝: 高拷貝
          啟動子:
          克隆方法: 限制性內切酶;多克隆位點
          載體大小: 2717 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽:
          載體抗性: 卡那霉素
          真核篩選標記:
          克隆菌株: DH5a 、TOP10感受態細胞
          備注: --
          產品目錄號: 11813-011
          病毒/非病毒: 非病毒

          pENTR1A載體質粒圖譜和多克隆位點信息

          pENTR 1A載體圖譜



          pENTR 1A多克隆位點

          pENTR1A載體簡介

          pENTR1A載體序列

          LOCUS       pENTR1A	2717 bp 	DNA	SYN
          DEFINITION  pENTR1A
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..2717
                               /organism="pENTR1A"
                               /mol_type="other DNA"
               terminator      106..149
                               /label="rrnB_T1_terminator"
               terminator      281..308
                               /label="rrnB_T2_terminator"
               misc_feature    complement(358..441)
                               /label="attL2"
               misc_feature    complement(358..457)
                               /label="attL1"
               misc_feature    612..911
                               /label="ccdB"
               misc_feature    946..1045
                               /label="attL2"
               gene            1168..1977
                               /label="KanR2 (variant)"
                               /gene="KanR2 (variant)"
               CDS             1168..1977
                               /label="ORF frame 1"
                               /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                               PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                               TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                               ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                               GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
               rep_origin      2051..2670
                               /label="pBR322_origin"
                               /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                               PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                               TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                               ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                               GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
          ORIGIN
              1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
             61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
            121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
            181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
            241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
            301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
            361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
            421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTTTA AAGGAACCAA TTCAGTCGAC
            481 TGGATCCGGT ACCGAATTCG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT
            541 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT
            601 GTGCTTCTAG AATGCAGTTT AAGGTTTACA CCTATAAAAG AGAGAGCCGT TATCGTCTGT
            661 TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG ACGGATAGTG ATCCCCCTGG
            721 CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT TTACCCGGTG GTGCATATCG
            781 GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG TGTGCCGGTC TCCGTTATCG
            841 GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT CAAAAACGCC ATTAACCTGA
            901 TGTTCTGGGG AATATAGAAT TCGCGGCCGC ACTCGAGATA TCTAGACCCA GCTTTCTTGT
            961 ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA
           1021 TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGC TCTGGCCCGT GTCTCAAAAT
           1081 CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT
           1141 TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG
           1201 CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC
           1261 GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT
           1321 CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC
           1381 TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT
           1441 GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT
           1501 CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT CCCTGCGCCG GTTGCATTCG
           1561 ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA
           1621 TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG
           1681 CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC
           1741 GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT
           1801 TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG
           1861 AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT
           1921 GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA
           1981 GAATTGGTTA ATTGGTTGTA ACATTATTCA GATTGGGCCC CGTTCCACTG AGCGTCAGAC
           2041 CCGGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC
           2101 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA
           2161 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA
           2221 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT
           2281 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG
           2341 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC
           2401 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA
           2461 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG
           2521 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT
           2581 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG
           2641 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG
           2701 CCTTTTGCTC ACATGTT
          //
          
          

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