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          pDONR221-P1P4

          價格:6500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Invitrogen
          載體名稱: pDONR221-P1P4 、pDONR221 P1-P4
          質粒類型: Mltisite-Gateway載體;DONR載體
          高拷貝/低拷貝: 低拷貝
          啟動子:
          克隆方法: Gateway;BP反應
          載體大小: 4774bp
          5' 測序引物及序列: M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3'
          3' 測序引物及序列: M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3'
          載體標簽:
          載體抗性: 卡那霉素,氯霉素(僅空載體)
          真核篩選標記:
          克隆菌株: 陽性篩選用DB3.1 ,陰性篩選用OmniMAX2-T1
          備注:
          pDONR221-P1P4載體是MultiSite Gateway載體,

          是研究啟動子的理想工具。
           
          產品目錄號: 2537-023
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pDONR221-P1P4載體圖譜



          pDONR221-P1P4多克隆位點

          pDONR221-P1P4 載體特征

          載體簡介

          pDONR221-P1P4使用方法

          載體序列

          LOCUS       Exported                4774 bp ds-DNA     circular SYN 10-AUG-2016
          DEFINITION  synthetic circular DNA
          KEYWORDS    pDONR221-P1P4
            AUTHORS   Biofeng Lab.
          FEATURES             Location/Qualifiers
               source          1..4774
                               /organism="synthetic DNA construct"
                               /mol_type="other DNA"
               terminator      268..295
                               /note="rrnB T2 terminator"
                               /note="transcription terminator T2 from the E. coli rrnB 
                               gene"
               terminator      387..473
                               /gene="Escherichia coli rrnB"
                               /note="rrnB T1 terminator"
                               /note="transcription terminator T1 from the E. coli rrnB 
                               gene"
               primer_bind     537..553
                               /note="M13 fwd"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               protein_bind    570..801
                               /gene="mutant version of attP"
                               /bound_moiety="BP Clonase(TM)"
                               /note="attP1"
                               /note="recombination site for the Gateway(R) BP reaction 
                               (pDONR(TM)201 version)"
               CDS             complement(1197..1502)
                               /codon_start=1
                               /gene="ccdB"
                               /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                               /note="ccdB"
                               /note="Plasmids containing the ccdB gene cannot be 
                               propagated in standard E. coli strains."
                               /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                               VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
               CDS             complement(1852..2505)
                               /codon_start=1
                               /gene="cat"
                               /product="chloramphenicol acetyltransferase"
                               /note="CmR"
                               /note="confers resistance to chloramphenicol"
                               /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                               KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                               LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                               DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
               promoter        complement(2506..2608)
                               /note="cat promoter"
                               /note="promoter of the E. coli cat gene encoding 
                               chloramphenicol acetyltransferase"
               protein_bind    complement(2753..2984)
                               /gene="mutant version of attP"
                               /bound_moiety="BP Clonase(TM)"
                               /note="attP4"
                               /note="recombination site for the Gateway(R) BP reaction"
               promoter        complement(3016..3034)
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               primer_bind     complement(3039..3055)
                               /note="M13 rev"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               CDS             3168..3977
                               /codon_start=1
                               /gene="aph(3')-Ia"
                               /product="aminoglycoside phosphotransferase"
                               /note="KanR"
                               /note="confers resistance to kanamycin in bacteria or G418 
                               (Geneticin(R)) in eukaryotes"
                               /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                               DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                               FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                               FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                               RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
               rep_origin      4124..4712
                               /direction=RIGHT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
          ORIGIN
                  1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
                 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
                121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
                181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
                241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
                301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
                361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
                421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
                481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
                541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
                601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
                661 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
                721 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt
                781 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct
                841 gccaacttag tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca
                901 gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg
                961 tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt
               1021 catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta
               1081 caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt
               1141 ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat
               1201 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
               1261 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
               1321 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc
               1381 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc
               1441 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
               1501 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
               1561 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
               1621 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
               1681 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
               1741 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
               1801 acgcatactg ttatctggct tttagtaagc cggatccacg cggcgtttac gccccccctg
               1861 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
               1921 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
               1981 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
               2041 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
               2101 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
               2161 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
               2221 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
               2281 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
               2341 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
               2401 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
               2461 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
               2521 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
               2581 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
               2641 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
               2701 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagtcg actacaggtc
               2761 actaatacca tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta
               2821 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
               2881 ttctcgttca acttttctat acaaagttgg cattataaga aagcattgct tatcaatttg
               2941 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagg
               3001 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
               3061 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
               3121 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
               3181 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
               3241 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
               3301 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
               3361 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
               3421 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
               3481 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
               3541 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
               3601 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
               3661 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
               3721 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
               3781 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
               3841 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
               3901 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
               3961 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
               4021 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
               4081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
               4141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
               4201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
               4261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
               4321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
               4381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
               4441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
               4501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
               4561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
               4621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
               4681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
               4741 tcctggcctt ttgctggcct tttgctcaca tgtt
          //
          
          

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