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          pDONR-P2RP3

          價格:6500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          --
          出品公司: Invitrigen
          載體名稱: pDONR-P2RP3 、pDONR P2R-P3
          質粒類型: Gateway載體;DONR載體
          高拷貝/低拷貝: 低拷貝
          啟動子:
          克隆方法: Gateway;BP反應
          載體大小: 4773bp
          5' 測序引物及序列: M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3'
          3' 測序引物及序列: M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3'
          載體標簽:
          載體抗性: 卡那霉素,氯霉素(僅空載體)
          真核篩選標記:
          克隆菌株: 陽性篩選用DB3.1 ,陰性篩選用OmniMAX2-T1
          備注:
          pDONR-P2RP3載體是MultiSite Gateway載體, 與pDONR221、pDONR-P4P1R載體一同構成三片段系統,

          目標載體是pDEST-R4R3,是研究啟動子的理想工具。
           
          產品目錄號: 2537-023
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pDONR-P2RP3載體圖譜



          pDONR-P2RP3多克隆位點

          載體簡介

          pDONR-P2RP3使用方法

          載體序列

          LOCUS       Exported                4773 bp ds-DNA     circular SYN 10-AUG-2016 .
          KEYWORDS    pDONR-P2RP3
          REFERENCE   1  (bases 1 to 4773)
            AUTHORS   Biofeng Lab.
          FEATURES             Location/Qualifiers
               source          1..4773
                               /organism="synthetic DNA construct"
                               /mol_type="other DNA"
               terminator      268..295
                               /note="rrnB T2 terminator"
                               /note="transcription terminator T2 from the E. coli rrnB 
                               gene"
               terminator      387..473
                               /gene="Escherichia coli rrnB"
                               /note="rrnB T1 terminator"
                               /note="transcription terminator T1 from the E. coli rrnB 
                               gene"
               primer_bind     537..553
                               /note="M13 fwd"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               protein_bind    complement(591..822)
                               /gene="mutant version of attP"
                               /bound_moiety="BP Clonase(TM)"
                               /note="attP2"
                               /note="recombination site for the Gateway(R) BP reaction 
                               (pDONR(TM)201 version)"
               promoter        980..1082
                               /note="cat promoter"
                               /note="promoter of the E. coli cat gene encoding 
                               chloramphenicol acetyltransferase"
               CDS             1083..1742
                               /codon_start=1
                               /gene="cat"
                               /product="chloramphenicol acetyltransferase"
                               /note="CmR"
                               /note="confers resistance to chloramphenicol"
                               /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                               KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                               LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                               DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
               CDS             2084..2389
                               /codon_start=1
                               /gene="ccdB"
                               /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                               /note="ccdB"
                               /note="Plasmids containing the ccdB gene cannot be 
                               propagated in standard E. coli strains."
                               /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                               VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
               protein_bind    complement(2746..2977)
                               /gene="mutant version of attP"
                               /bound_moiety="BP Clonase(TM)"
                               /note="attP3"
                               /note="recombination site for the Gateway(R) BP reaction"
               promoter        complement(3015..3033)
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               primer_bind     complement(3038..3054)
                               /note="M13 rev"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               CDS             3167..3976
                               /codon_start=1
                               /gene="aph(3')-Ia"
                               /product="aminoglycoside phosphotransferase"
                               /note="KanR"
                               /note="confers resistance to kanamycin in bacteria or G418 
                               (Geneticin(R)) in eukaryotes"
                               /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                               DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                               FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                               FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                               RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
               rep_origin      4123..4711
                               /direction=RIGHT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
          ORIGIN
                  1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
                 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
                121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
                181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
                241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
                301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
                361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
                421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
                481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
                541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
                601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
                661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
                721 ctcgttcaac tttcttgtac aaagttggca ttataaaaaa gcattgctta tcaatttgtt
                781 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggagctct agagcgtcga
                841 ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg acggaagatc
                901 acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact
                961 tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa
               1021 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
               1081 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
               1141 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
               1201 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
               1261 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
               1321 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
               1381 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
               1441 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
               1501 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
               1561 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
               1621 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc
               1681 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
               1741 aatcgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
               1801 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc
               1861 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
               1921 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
               1981 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
               2041 gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta
               2101 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
               2161 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
               2221 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
               2281 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
               2341 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
               2401 ccttatacac agccagtctg caggtcgata cagtagaaat tacagaaact ttatcacgtt
               2461 tagtaagtat agaggctgaa aatccagatg aagccgaacg acttgtaaga gaaaagtata
               2521 agagttgtga aattgttctt gatgcagatg attttcagga ctatgacact agcgtatatg
               2581 aataggtaga tgtttttatt ttgtcacaca aaaaagaggc tcgcacctct ttttcttatt
               2641 tctttttatg atttaatacg gcattgagga caatagcgag taggctggat acgacgattc
               2701 cgtttgagaa gaacatttgg aaggctgtcg gtcgagctcg aattctacag gtcactaata
               2761 ccatctaagt agttgattca tagtgactgc atatgttgtg ttttacagta ttatgtagtc
               2821 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
               2881 tcaactttat tatacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac
               2941 gaacaggtca ctatcagtca aaataaaatc attatttgga gctccatggt agcgttaacg
               3001 cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
               3061 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
               3121 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
               3181 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
               3241 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
               3301 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
               3361 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
               3421 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
               3481 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
               3541 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
               3601 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
               3661 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
               3721 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
               3781 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
               3841 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
               3901 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
               3961 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
               4021 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
               4081 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
               4141 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
               4201 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
               4261 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
               4321 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
               4381 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
               4441 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
               4501 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
               4561 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
               4621 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
               4681 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
               4741 cctggccttt tgctggcctt ttgctcacat gtt
          //
          
          

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