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          pgRNA-bacteria

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: 愛迪基因
          載體名稱: pgRNA-bacteria
          質粒類型: CRISPR/Cas9載體;細菌基因敲除載體
          高拷貝/低拷貝: 高拷貝
          插入位點: 限制性內切酶,多克隆位點
          啟動子: J23119(SpeI)
          載體大小: 2584 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: mRFP (N-ter)
          載體抗性: Ampicillin
          克隆菌株: Top10
          宿主細胞(系): 細菌,如大腸桿菌
          備注:  
          產品目錄號: #44251
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pgRNA-bacteria 載體圖譜



          pgRNA-bacteria 多克隆位點

          載體簡介

          載體序列

          LOCUS       pgRNA-bacteria	2584 bp 	DNA	SYN
          DEFINITION  pgRNA-bacteria
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..2584
                               /organism="pgRNA-bacteria"
                               /mol_type="other DNA"
               misc_feature    complement(285..302)
                               /label="pTrcHis_rev_primer"
               misc_feature    complement(285..302)
                               /label="pBAD_rev_primer"
               terminator      335..492
                               /label="rrnB_terminator"
               terminator      458..501
                               /label="rrnB_T1_terminator"
               gene            complement(1484..2344)
                               /label="Ampicillin"
                               /gene="Ampicillin"
               CDS             complement(1484..2344)
                               /label="ORF frame 1"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
               promoter        complement(2386..2414)
                               /label="AmpR_promoter"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 gAATTCTAAA GATCTTTGAC AGCTAGCTCA GTCCTAGGTA TAATACTAGT AACTTTCAGT
             61 TTAGCGGTCT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC
            121 TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTTGAAGCTT GGGCCCGAAC AAAAACTCat
            181 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac
            241 ggtctccagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
            301 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
            361 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
            421 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
            481 agactGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC TGGATCCTTA CTCGAGTCTA
            541 GACTGCAGgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
            601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
            661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
            721 cgtttttcca Caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
            781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
            841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
            901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
            961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
           1021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
           1081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
           1141 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
           1201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
           1261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
           1321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
           1381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
           1441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
           1501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
           1561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
           1621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
           1681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
           1741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
           1801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
           1861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
           1921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
           1981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
           2041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
           2101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
           2161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
           2221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
           2281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
           2341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
           2401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
           2461 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
           2521 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
           2581 tctg
          //
          

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