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          pCas-Guide-EF1a-GFP

          價格:2000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: ORIGENE
          載體名稱: pCas-Guide-EF1a-GFP
          質粒類型: CRISPR/Cas9載體;基因敲除載體;哺乳動物細胞載體
          高拷貝/低拷貝: 高拷貝
          插入位點: 限制性內切酶,多克隆位點
          啟動子: U6
          載體大小: 10493 bp
          5' 測序引物及序列: 5’ gatcgATGGGAGGTGGTATGGGAGGg 3’
          3' 測序引物及序列: 5’ aaaacCCTCCCATACCACCTCCCATc 3’
          載體標簽: Myc(C-ter)
          載體抗性: 氨芐青霉素
          篩選標記: GFP
          克隆菌株: Stbl3
          宿主細胞(系): 哺乳動物細胞
          備注: --
          產品目錄號: GE100018
          穩定性: 瞬表達或穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pCas-Guide-EF1a-GFP載體圖譜



          載體簡介

          pCas-Guide-EF1a-GFP
          
          All-in-one vector
          Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
          BamH I and BsmB I can be used for genomic target sequence cloning
          Expresses gRNA containing the inserted target sequence and the gRNA scaffold
          GFP as a reporter for tracking or sorting transfected cells
          CF3 primer can be used to sequence the target sequence after cloning
          

          載體序列

          LOCUS       Exported File          10493 bp ds-DNA    circular SYN 23-七月-2016
          DEFINITION  Mammalian vector for co-expressing a guide RNA (gRNA) together with 
                      Cas9 and TurboGFP, for genome editing using the S. pyogenes 
                      CRISPR/Cas9 system.
          ACCESSION   .
          VERSION     .
          KEYWORDS    pCas-Guide-EF1a-GFP
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 10493)
            AUTHORS   OriGene
            TITLE     Direct Submission
            JOURNAL   Exported 2016-07-23 from SnapGene Viewer 1.5.3
                      http://www.snapgene.com
          FEATURES             Location/Qualifiers
               source          1..10493
                               /organism="synthetic DNA construct"
                               /lab_host="Mammalian Cells"
                               /mol_type="other DNA"
               promoter        90..330
                               /note="U6"
                               /note="RNA polymerase III promoter for human U6 snRNA"
                               /note="color: #000000; direction: RIGHT"
               misc_RNA        384..459
                               /note="gRNA scaffold"
                               /note="guide RNA scaffold for the Streptococcus pyogenes 
                               CRISPR/Cas9 system"
                               /note="color: #00ccff; direction: RIGHT"
               terminator      460..465
                               /note="polIII terminator"
                               /note="RNA polymerase III transcription terminator"
                               /note="color: #ffffff"
               promoter        480..1658
                               /note="EF-1-alpha promoter"
                               /note="strong constitutive promoter for human elongation 
                               factor EF-1-alpha"
                               /note="color: #000000; direction: RIGHT"
               intron          711..1649
                               /note="EF-1-alpha intron A"
                               /note="intron upstream of the start codon of human 
                               EF-1-alpha"
                               /note="color: #------"
               CDS             1737..2432
                               /codon_start=1
                               /product="improved green fluorescent protein from 
                               Pontellina plumata?(Evdokimov et al., 2006)"
                               /note="TurboGFP"
                               /note="mammalian codon-optimized"
                               /note="color: #05fd14"
                               /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                               KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                               HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                               TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                               KTPDADAGEE"
               enhancer        3047..3350
                               /note="CMV enhancer"
                               /note="human cytomegalovirus immediate early enhancer"
                               /note="color: #000000"
               promoter        3351..3554
                               /note="CMV promoter"
                               /note="human cytomegalovirus (CMV) immediate early 
                               promoter"
                               /note="color: #000000; direction: RIGHT"
               promoter        3580..3598
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
                               /note="color: #ffffff; direction: RIGHT"
               CDS             3657..7760
                               /codon_start=1
                               /locus_tag="
                               "
                               /product="Cas9 (Csn1) endonuclease from the?Streptococcus 
                               pyogenes?Type II CRISPR/Cas system"
                               /note="Cas9"
                               /note="generates RNA-guided double strand breaks in DNA"
                               /note="color: #993366"
                               /protein_id="
                               "
                               /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                               NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                               FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                               FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                               ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                               IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                               QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                               RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                               NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                               FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                               SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                               LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                               QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                               HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                               LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                               SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                               VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                               NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                               EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                               ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                               TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                               LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                               ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                               DATLIHQSITGLYETRIDLSQLGGD"
               CDS             7761..7781
                               /codon_start=1
                               /locus_tag="
                               "
                               /product="nuclear localization signal of SV40 large T 
                               antigen"
                               /note="SV40 NLS"
                               /note="
                               "
                               /note="color: #993366"
                               /protein_id="
                               "
                               /translation="PKKKRKV"
               CDS             7788..7808
                               /codon_start=1
                               /locus_tag="
                               "
                               /product="nuclear localization signal of SV40 large T 
                               antigen"
                               /note="SV40 NLS"
                               /note="
                               "
                               /note="color: #993366"
                               /protein_id="
                               "
                               /translation="PKKKRKV"
               CDS             7830..7859
                               /codon_start=1
                               /product="Myc (human c-Myc oncogene) epitope tag"
                               /note="Myc"
                               /note="color: #cc99b2"
                               /translation="EQKLISEEDL"
               CDS             7878..7901
                               /codon_start=1
                               /product="FLAG(R) epitope tag, followed by an enterokinase 
                               cleavage site"
                               /note="FLAG"
                               /note="color: #cc99b2"
                               /translation="DYKDDDDK"
               polyA_signal    7948..8570
                               /note="hGH poly(A) signal"
                               /note="human growth hormone polyadenylation signal"
                               /note="color: #a6acb3"
               rep_origin      complement(8691..9279)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
                               /note="color: #ffff00"
               CDS             complement(9454..10314)
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /note="This feature has 2 segments:
                               ???1:??9454?..?10245?/?#cc99ff
                               ???2:?10246?..?10314?/?#cc99ff?/?signal?sequence
                               Cleavage site after base 10245"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
          ORIGIN
                  1 gaattcccca gtggaaagac gcgcaggcaa aacgcaccac gtgacggagc gtgaccgcgc
                 61 gccgagcgcg cgccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
                121 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
                181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
                241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
                301 tcttgggttt atatatcttg tggaaaggac gcgggatcca ctggaccagg cagcagcgtc
                361 agaagacttt tttggaacgt ctcgttttag agctagaaat agcaagttaa aataaggcta
                421 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggtg tacacgtgag
                481 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
                541 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
                601 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
                661 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
                721 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
                781 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
                841 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
                901 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
                961 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
               1021 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
               1081 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
               1141 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
               1201 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
               1261 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
               1321 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
               1381 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
               1441 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
               1501 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
               1561 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
               1621 tggttcaaag tttttttctt ccatttcagg tgtcgtgact atagggcggc cggacgtgac
               1681 aaatggaagt agcacgcctc actaggctcg tgcagatgga cagcaccgct gcagccatgg
               1741 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga
               1801 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccgagcag ggccgcatga
               1861 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg
               1921 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc
               1981 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg
               2041 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca
               2101 aggtgatggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca
               2161 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgatctggat ggcagcttca
               2221 cccgcacctt cagcctgcgc gacggcggct actacagctc cgtggtggac agccacatgc
               2281 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc
               2341 gccgcgtgga ggaggatcac agcaacaccg agctgggcat cgtggagtac cagcacgcct
               2401 tcaagacccc ggatgcagat gccggtgaag aaagagttta atcgatgata tcagatcccc
               2461 gggatgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat ggaagttttt
               2521 cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa tggaaggatt
               2581 ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt actgaaggct
               2641 ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa acaagcaaaa
               2701 ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata gatctctcgt
               2761 gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg gtttccaaat
               2821 gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat acacttcatt
               2881 ctcagtattg ttttgccaag ttctaattcc atcagaagct ggtcgagatc cggaaccctt
               2941 aatataactt cgtataatgt atgctatacg aagttattag gtccactagt tattaatagt
               3001 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
               3061 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
               3121 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
               3181 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
               3241 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
               3301 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
               3361 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
               3421 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
               3481 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
               3541 atataagcag agctcgttta gtgaaccgtc agaattttgt aatacgactc actatagggc
               3601 ggccgggaat tcgtcgactg gaaccggtac cgaggagatc tgccgccgcg atcgccatgg
               3661 ataagaaata ctcaatagga ctggatattg gcacaaatag cgtcggatgg gctgtgatca
               3721 ctgatgaata taaggttcct tctaaaaagt tcaaggttct gggaaataca gaccgccaca
               3781 gtatcaaaaa aaatcttata ggggctcttc tgtttgacag tggagagaca gccgaagcta
               3841 ctagactcaa acggacagct aggagaaggt atacaagacg gaagaatagg atttgttatc
               3901 tccaggagat tttttcaaat gagatggcca aagtggatga tagtttcttt catagacttg
               3961 aagagtcttt tttggtggaa gaagacaaga agcatgaaag acatcctatt tttggaaata
               4021 tagtggatga agttgcttat cacgagaaat atccaactat ctatcatctg agaaaaaaat
               4081 tggtggattc tactgataaa gccgatttgc gcctgatcta tttggccctg gcccacatga
               4141 ttaagtttag aggtcatttt ttgattgagg gcgatctgaa tcctgataat agtgatgtgg
               4201 acaaactgtt tatccagttg gtgcaaacct acaatcaact gtttgaagaa aaccctatta
               4261 acgcaagtgg agtggatgct aaagccattc tttctgcaag attgagtaaa tcaagaagac
               4321 tggaaaatct cattgctcag ctccccggtg agaagaaaaa tggcctgttt gggaatctca
               4381 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
               4441 ctaaactcca gctttcaaaa gatacttacg atgatgatct ggataatctg ttggctcaaa
               4501 ttggagatca atatgctgat ttgtttttgg cagctaagaa tctgtcagat gctattctgc
               4561 tttcagacat cctgagagtg aatactgaaa taactaaggc tcccctgtca gcttcaatga
               4621 ttaaacgcta cgatgaacat catcaagact tgactcttct gaaagccctg gttagacaac
               4681 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
               4741 atattgatgg cggcgcaagc caagaagaat tttataaatt tatcaaacca attctggaaa
               4801 aaatggatgg tactgaggaa ctgttggtga aactgaatag agaagatttg ctgcgcaagc
               4861 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
               4921 ttttgagaag acaagaagac ttttatccat ttctgaaaga caatagagag aagattgaaa
               4981 aaatcttgac ttttaggatt ccttattatg ttggtccatt ggccagaggc aatagtaggt
               5041 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
               5101 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
               5161 ttccaaatga aaaagtgctg ccaaaacata gtttgcttta tgagtatttt accgtttata
               5221 acgaattgac aaaggtcaaa tatgttactg aaggaatgag aaaaccagca tttctttcag
               5281 gtgaacagaa gaaagccatt gttgatctgc tcttcaaaac aaataggaaa gtgaccgtta
               5341 agcaactgaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
               5401 gagttgaaga tagatttaat gcttcactgg gtacatacca tgatttgctg aaaattatta
               5461 aagataaaga ttttttggat aatgaagaaa atgaagacat cctggaggat attgttctga
               5521 cattgaccct gtttgaagat agggagatga ttgaggaaag acttaaaaca tacgctcacc
               5581 tctttgatga taaggtgatg aaacagctta aaagacgcag atatactggt tggggaaggt
               5641 tgtccagaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atactggatt
               5701 ttttgaaatc agatggtttt gccaatcgca attttatgca gctcatccat gatgatagtt
               5761 tgacatttaa agaagacatc caaaaagcac aagtgtctgg acaaggcgat agtctgcatg
               5821 aacatattgc aaatctggct ggtagccctg ctattaaaaa aggtattctc cagactgtga
               5881 aagttgttga tgaattggtc aaagtgatgg ggcggcataa gccagaaaat atcgttattg
               5941 aaatggcaag agaaaatcag acaactcaaa agggccagaa aaattccaga gagaggatga
               6001 aaagaatcga agaaggtatc aaagaactgg gaagtcagat tcttaaagag catcctgttg
               6061 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagata
               6121 tgtatgtgga ccaagaactg gatattaata ggctgagtga ttatgatgtc gatcacattg
               6181 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcctgacc aggtctgata
               6241 aaaatagagg taaatccgat aacgttccaa gtgaagaagt ggtcaaaaag atgaaaaact
               6301 attggagaca acttctgaac gccaagctga tcactcaaag gaagtttgat aatctgacca
               6361 aagctgaaag aggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
               6421 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
               6481 aatacgatga aaatgataaa cttattagag aggttaaagt gattaccctg aaatctaaac
               6541 tggtttctga cttcagaaaa gatttccaat tctataaagt gagagagatt aacaattacc
               6601 atcatgccca tgatgcctat ctgaatgccg tcgttggaac tgctttgatt aagaaatatc
               6661 caaaacttga aagcgagttt gtctatggtg attataaagt ttatgatgtt aggaaaatga
               6721 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa gtatttcttt tactctaata
               6781 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
               6841 tgatcgaaac taatggggaa actggagaaa ttgtctggga taaagggaga gattttgcca
               6901 cagtgcgcaa agtgttgtcc atgccccaag tcaatatcgt caagaaaaca gaagtgcaga
               6961 caggcggatt ctctaaggag tcaattctgc caaaaagaaa ttccgacaag ctgattgcta
               7021 ggaaaaaaga ctgggaccca aaaaaatatg gtggttttga tagtccaacc gtggcttatt
               7081 cagtcctggt ggttgctaag gtggaaaaag ggaaatccaa gaagctgaaa tccgttaaag
               7141 agctgctggg gatcacaatt atggaaagaa gttcctttga aaaaaatccc attgactttc
               7201 tggaagctaa aggatataag gaagttaaaa aagacctgat cattaaactg cctaaatata
               7261 gtctttttga gctggaaaac ggtaggaaac ggatgctggc tagtgccgga gaactgcaaa
               7321 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tctgtatctg gctagtcatt
               7381 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
               7441 ataagcatta tctggatgag attattgagc aaatcagtga attttctaag agagttattc
               7501 tggcagatgc caatctggat aaagttctta gtgcatataa caaacataga gacaaaccaa
               7561 taagagaaca agcagaaaat atcattcatc tgtttacctt gaccaatctt ggagcacccg
               7621 ctgcttttaa atactttgat acaacaattg ataggaaaag atatacctct acaaaagaag
               7681 ttctggatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
               7741 tgagtcagct gggaggtgac cccaagaaaa aacgcaaggt ggaagatcct aagaaaaagc
               7801 ggaaagtgga cacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
               7861 cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaaacg gccggccgcg
               7921 gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct
               7981 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
               8041 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
               8101 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
               8161 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
               8221 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
               8281 taatttttgt ttttttggta gaggcggggt ttcaccatat tggccaggct ggtctccaac
               8341 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
               8401 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
               8461 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
               8521 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
               8581 ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
               8641 ggataacgca ggaaagaaca tgtccgtaaa aaggccgcgt tgctggcgtt tttccatagg
               8701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
               8761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
               8821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
               8881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
               8941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
               9001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
               9061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
               9121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
               9181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
               9241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
               9301 acggggtctg acgctcagtg gaacgacgcg taactcacgt taagggattt tggtcatgag
               9361 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
               9421 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
               9481 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
               9541 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
               9601 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
               9661 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
               9721 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
               9781 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
               9841 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
               9901 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
               9961 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
              10021 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
              10081 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
              10141 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
              10201 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
              10261 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
              10321 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgatga tattatttta
              10381 tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca ggaaacagct
              10441 atgaccatgt aatacgactc actatagggg atatcagctg gatggcagtt aac
          //
          
          

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